Co-culturing and Assaying Spheroids in the Corning® Spheroid Microplate
23 May 2017In this study, A549 cells, a human lung carcinoma cell line, were cultured in spheroid microplates with and without co-culture conditions, including primary human lung fibroblasts and peripheral blood mononuclear cells. Tumor spheroid viability was screened after treatment with various chemotherapeutics in both single culture and co-culture conditions. Dose-dependent responses of selected chemotherapeutics were compared, demonstrating the impact that including multiple cell types in 3D assays can have on therapeutic outcome.
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Corning® 384-well Black/Clear Round Bottom Ultra-Low Attachment Spheroid Microplate, Bulk Packed 10 per Bag, with Lid, Sterile
Corning Life SciencesUltra-Low Attachment microplates feature a covalently bound hydrogel layer that effectively inhibits cellular attachment Surface minimizes protein absorption, enzyme activation and cellular activation Surface is noncytotoxic, biologically inert and nondegradable Sterilized by gamma irradiation Black wall microplates have low background fluorescence, minimal light scatter and reduced crosstalk Opaque walls to prevent well-to-well crosstalk Can be used for both top and bottom reading instruments Bulk packed 10 per bag Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body