Cancer Research Workflow Pinpointing the Appropriate Time Points for Compound Testing Using Nutlin-Induced Apoptosis as a Model

This application note presents a study that describes a model system of the cellular effects of nutlin 3a and nutlin 3b using continuous cell recording with the xCELLigence System and qRT-PCR analysis using the LightCycler® 480 Real-Time PCR System. In addition, it provides insight into nutlin-induced cellular events by various endpoint assays for cell viability, proliferation and apoptosis/necrosis. The proposed workflow is a powerful model system for cellular analysis, pinpointing the optimal time points for functional endpoint assays and qPCR.