ResourceLife Sciences
Blocking guide
20 Feb 2026Effective immunohistochemistry and immunofluorescence rely on minimizing background noise caused by endogenous enzymes, biotin, immunoglobulins, cross‑reactivity, and autofluorescence. This guide explains the biological sources of these issues and outlines when and why blocking steps are essential for achieving clear, high‑contrast staining.
Learn practical blocking strategies, reagent selection tips, and troubleshooting guidance that help researchers reduce non‑specific signals, improve signal‑to‑noise ratios, and generate more reliable, interpretable IHC and IF results.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell Staining