Blocking guide

Effective immunohistochemistry and immunofluorescence rely on minimizing background noise caused by endogenous enzymes, biotin, immunoglobulins, cross‑reactivity, and autofluorescence. This guide explains the biological sources of these issues and outlines when and why blocking steps are essential for achieving clear, high‑contrast staining.

Learn practical blocking strategies, reagent selection tips, and troubleshooting guidance that help researchers reduce non‑specific signals, improve signal‑to‑noise ratios, and generate more reliable, interpretable IHC and IF results.