ResourceLab Automation
Automating complex manual sample preparation for mass spec-based proteomics using the Opentrons OT-2 platform
4 Oct 2024As quantitative proteomics experiments increase in scale and complexity, greater consistency and precision are required, which is best delivered by automation. Dr. Joao Paulo and his laboratory at the Harvard Medical School Department of Cell Biology employ mass spectrometry-based proteomics (MSP) analysis to better understand the effects of molecular mechanisms and cell signaling pathways on the regulation of the proteome of various model systems. He sought to replace labor-intensive manual sample preparation with automation, and his work serves as a demonstrated case study for successful automation of sample preparation for mass spectrometry-based proteomics using the Opentrons OT-2.
Related products
Request Quote for All Products
Links
Tags
ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Automation Supplies / AccessoriesAutomated accessories and consumables make lab processes fast, efficient and reliable. Useful automation accessories include microplate sealers, label printers and reagent reservoirs.Mass SpectrometryMass spectrometry (MS) is a powerful analytical technique used to identify and quantify molecules based on the mass-to-charge ratio of gas-phase ions. It provides detailed information about the structure, composition, and properties of compounds and is widely used across fields such as environmental monitoring, materials science, drug discovery and development, food and beverage testing, and wider chemical research. Key MS techniques include tandem mass spectrometry (MS/MS), liquid chromatography–mass spectrometry (LS-MS) and inductively coupled plasma (ICP-MS). Choosing from these wide range of techniques and technologies can be a daunting task, so keep up to date with scientific applications, performance expectations, and customer reviews here all in one place. Visit our product directory to receive quotes direct from the manufacturer.