Automated Size Selection of NEBNext® Small RNA Libraries with the Sage Pippin Prep
18 Aug 2014This application note demonstrates an optimized Pippin Prep protocol for use with the NEBNext Small RNA library kits. The Pippin Prep protocol provides all of the sequence quality benefits of the standard manual gel protocol, but with greatly enhanced ease-of-use and reproducibility.
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Pippin Prep DNA Size Selection System
Sage ScienceThe Pippin Prep is a preparative electrophoresis platform for collection of size-selected DNA samples. The system automates DNA size selection using disposable, pre-cast agarose cassettes to extract fractions according to user-defined software input. Samples are electro-eluted from agarose and collected in buffer. The platform is used for the construction of next-gen sequencing libraries. The system collects fragments (depending on gel type) between 90 bp- 8kb. A higher quality of sample can be extracted, more reproducibly and with no cross-contamination, when compared to manual gel purification methods.