Automated single-cell cloning of CHO-S cells using animal-component free conditions in nanolitre volumes
5 Aug 2024Cell line development (CLD) aims to engineer cell lines that produce recombinant proteins and antibodies with high titres, stringent quality and highest specificity. A critical yet challenging and rate limiting step as part of CLD is the generation of clonal cultures verifiably originating from a single cell. Single-cell cloning remains a tedious, lengthy and inefficient process. Chinese Hamster Ovary (CHO) cells are the main cell type utilized for CLD projects. Explore iotaSciences' assessment of the Cloning Platform for the automated cloning of single CHO cells under animal-component free (ACF) culture media conditions using only nanolitre volumes, while providing whole chamber images of single cells to verify monoclonality of derived cultures.
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isoCell – Single Cell Cloning Automation
iotaSciencesThe isoCell helps automate your single-cell cloning experiments without complex protocols. This instrument miniturises cell culture volumes and almost eliminates all manual pipetting steps. Utilising our GRID technology, users can confidently confirm monoclonality on day 0. With a compact footprint and easy-to-use interface, the isoCell is one of the smallest but most powerful instruments for single-cell cloning available.
Cloning Platform – Single Cell Cloning and Imaging
iotaSciencesThe Cloning Platform is a complete workflow solution for single-cell cloning. It offers automated handling of miniturised culture volumes as low as 200 nL and easy confirmation of monoclonality on day 0. Gentle handling with built-in programs for plating cells, feeding colonies, and harvesting clones maximises cloning efficiencies. With imaging and fluorescence options available, there's a configuration to suit your needs.