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Automated Extraction and Determination of Human Hormones in Drinking Water Using Solid-Phase Extraction and HPLC with UV Detection

3 Apr 2014

This Technical Note describes the use of high-surface-area SPE (Dionex SolEx SPE HRPHS) to extract seven sex hormones from drinking water using the Dionex AutoTrace 280 SPE instrument. Extracts were subsequently quantified using high-performance liquid chromatography (HPLC) to determine the recovery.

Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction Instrument

Thermo Fisher Scientific

The Thermo Scientific™ Dionex™ AutoTrace™ 280 Solid-Phase Extraction (SPE) instrument is a powerful, high-throughput workstation dedicated specifically for automating SPE. The Dionex AutoTrace 280 instrument automates the cartridge or disk conditioning, sample loading, rinsing, and eluting steps for large volume aqueous solution extractions (20 mL to 20 L).The unit can process up to six samples in parallel, automatically conditioning, washing, and eluting SPE cartridges or disks with a choice of up to five reagents. The unit also segregates aqueous and solvent waste using separate waste lines for efficient, cost effective waste handling.AutoTrace 280 Solid-Phase Extraction Instrument Features: Use SPE technology for liquid-liquid extraction Automated sample loading of liquids onto SPE cartridges Automated eluting of SPE cartridges with organic solvent Closed systems with a fan to vent solvent vapors Positive pressure loading and elution of samples and solvents Benefits: Reduced solvent usage and elimination of glassware Does not require technician involvement to maintain a reservoir of liquid in the SPE cartridge Does not require technician involvement to control the flow of organics solvent through the SPE cartridge No hood required Provides constant flow of liquids through SPE cartridges

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Automated Extraction and Determination of Human Hormones in Drinking Water Using Solid-Phase Extraction and HPLC with UV Detection