ResourceLife Sciences
Automated Cell Synchrony Determination Using Microscopic Imaging in Live Cells
9 Mar 2015The effect of compounds on cellular proliferation is a key element of the ADME/Tox drug discovery process. For this reason the assessment of cell cycle progression under experimental conditions is highly desirable. This poster demonstrates that cells actively duplicating their DNA (S-phase) can be identified using incorporation of EdU into cellular DNA with subsequent labeling with fluorescent tags via Click-iT® chemistry and imaging with the Cytation™ 3 multimode reader.
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Cell Cycle Analysis