Applying Improved TOF Mass Resolving Capability to Enhance the Characterization of Therapeutic Antibodies in Middle-Up and –Down Workflows

This poster demonstrates a method to extend the specificity of intact mass analysis and Middle-Down sequencing by deploying MD MALDI-ISD for high quality identification of sequence variations in combination with a new ultrahigh resolution (UHR)-ESI QTOF providing monoisotopic mass determinations of HCs with no speed or dynamic range compromise.