Androgen Receptor Controls EGFR and ERBB2 Gene Expression at Different Levels in Prostate Cancer Cell Lines

X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines such as HT29, a human adenocarcinoma cell line. When used for cotransfection-based gene knockdown, X-tremeGENE siRNA Transfection Reagent enables significant protein expression and effective knockdown levels due to its high transfection efficiency. The X-tremeGENE siRNA Transfection Reagent is intended for research applications only. For the most current list of successfully transfected cell types, visit www.powerful-transfection.com. The X-tremeGENE siRNA Transfection Reagent delivers all the benefits of high transfection efficiency, low cytotoxicity, and the capability of transfecting a broad range of cell types. Perform effective gene knockdown: Transfect with high efficiency to study the cellular and functional consequences of gene silencing – achieve over 90% gene knockdown in many different cell types. Maximize flexibility: Use a single reagent for siRNA- and co-transfection-based gene-knockdown experiments. Generate meaningful results: Employ a reagent with minimal cellular cytotoxicity to ensure that observed cellular effects are due to the transfected siRNA and not the transfection procedure. Benefit from an easy-to-use reagent: Effectively silence genes in just two easy steps, increase your productivity, and extend your resources.

Application The Cell Proliferation Reagent WST-1 is designed to be used for the nonradioactive, spectrophotometric quantification of cell proliferation and viability in cell populations using the 96-well-plate format. It can be used for: Measurement of cell proliferation in response to growth factors, cytokines, mitogens, and nutrients Analysis of cytotoxic and cytostatic compounds, such as anti-cancer drugs and other pharmaceutical compounds Assessment of growth inhibitory antibodies and physiological mediators Benefits Safe: No radioactive isotopes are used. Accurate: The absorbance revealed strongly correlates to the cell number. Sensitive: Low cell numbers are detected. Fast: The use of a multiwell ELISA reader allows a larger number of samples to be processed. Easy: No washing steps or additional reagents are required. Convenient: Supplied as a ready-to-use solution.

Protease Inhibitor Cocktail Tablets supplied in EASYpacks Application Used for the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts in small volumes. Benefits Simplify protection. Proteins are protected against a multitude of proteases by simply adding a Complete Tablet to aqueous protein isolation buffer. Use a convenient tablet. Tablets combining different protease inhibitors, with or without EDTA, are available for either 10 ml or 50 ml of lysate. Eliminate weighing small amounts. Sufficient amounts of various inhibitors are conveniently supplied in a tablet form. Insist on a versatile product. Proteases are inhibited in extracts from almost any tissue or cell type, including animals, plants, yeast, bacteria, or fungi. Product Description Starting concentration: 1 tablet contains protease inhibitors sufficient for a 10-ml cell extract. Specificity: Mixture of several protease inhibitors with broad inhibitory specificity. For the inhibition of serine, cysteine, and metalloproteases in bacterial, mammalian, yeast, and plant cell extracts. Solubility/Stability: Soluble in aqueous buffers, or add directly to extraction media. Alternatively, prepare 7x stock solutions in 1.5 ml water or 100 mM phosphate buffer, pH 7.0. Stock solution is stable for 1-2 weeks at +2 to +8°C or at least 12 weeks at -15 to -25°C. Can be used in thiol-containing solutions at room temperature. Note: Inhibition efficiency is quickly and sensitively tested with the Universal Protease Substrate (Cat. Nos. 11 080 733 001 or 11 734 334 001).