Analytical Performance of Micro Pillar Array Columns (μPAC™) for Metabolic Profiling
24 Jan 2018Metabolic profiling studies have become indispensable in a wide range of research fields, they allow profound insights into the biochemistry of an organism at a certain moment. Liquid chromatography (LC) and mass spectrometry have become vital tools in these studies and more sensitive mass spectrometers alongside new or improved LC techniques allow scientists to retrieve more information from minute sampling amounts. In this study, plant extract samples are used to compare the analytical performance of a µPAC™ nano LC column to state-of-the-art ultra performance LC columns.
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200 cm µPAC™ column
PharmaFluidicsAs an alternative to conventional columns, PharmaFluidics offers micromachined nano-LC columns or micro Pillar Array Columns (µPAC™). These µPAC™ columns display exceptional separation properties with unprecedented peak capacity and narrow peak shapes at low operating back-pressure. µPAC™ column, 200 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.
50 cm µPAC™ column
PharmaFluidicsWith an internal volume of 3µL, this 50 cm long RP-C18 µPAC™ column is perfectly suited to perform high throughput analyses with shorter gradient solvent times (30, 60 and 90-minute gradients) and it can be used over a wide range of flow rates, between 100 and 2000 nL/min. µPAC™ column, 50 cm length, with pillar array backbone at interpillar distance of 2.5µm, C18.