Analysis of Methylimidazoles in Caramel Colored Carbonated Beverages

21 Oct 2014

This application note describes the use of an Ascentis Express HILIC column as a unique approach for analysis of the methylimidazole compounds. The direct analysis technique simplifies the sample workflow, and combined with HILIC chromatographic conditions, results in a simple and fast analytical method to determine the levels of 2-methylimidazole and 4-methylimidazole in caramel colored carbonated beverages.

Ascentis® Express HILIC 2.7 Micron HPLC Column

Sigma-Aldrich Supelco

Ascentis Express HPLC columns, through the use of Fused-Core® particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower backpressures. The combination of high efficiency and low backpressure benefits UPLC® (or other ultra high pressure system) users, as well as conventional HPLC users. Column Details: Mode of chromatography: Hydrophilic interaction Matrix active group: HILIC phase Particle size: 2.7 μm Pore size: 90 Å Length: 100 mm ID: 2.1 mm

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Analysis of Methylimidazoles in Caramel Colored Carbonated Beverages