Amplification of Long Products from Human Genomic DNA

PrimeSTAR GXL DNA Polymerase is a high fidelity polymerase that provides efficient PCR amplification even in challenging situations, such as when amplifiying GC-rich templates, when there is eXcess template in the reaction mixture, and when amplifying Long products up to 30 kb (GXL). PrimeSTAR GXL Polymerase is the most robust high fidelity polymerase available, routinely allowing amplification of PCR products that cannot be amplified by other enzymes. PrimeSTAR GXL DNA Polymerase saves time by minimizing optimization efforts, amplifying targets regardless of the conditions.The PrimeSTAR GXL kit includes buffer and dNTPs.PrimeSTAR GXL DNA Polymerase is a modified version of PrimeSTAR HS enzyme that includes an elongation factor. This combination enables unsurpassed processivity. PrimeSTAR GXL DNA Polymerase displays outstanding performance in reactions containing excessive nucleic acid. Extraneous DNA ordinarily inhibits amplification by conventional polymerases because the amount of effective polymerase is limited by nonspecific binding. Thanks to the superior processivity of PrimeSTAR GXL DNA Polymerase, inhibition by excessive nucleic acid is limited, resulting in a much higher PCR success rate.Features: Buffer and dNTPs are conveniently provided in the PrimeSTAR GXL kit Highest processivity of all commercially available high-fidelity DNA polymerases Antibody-mediated hot-start formulation Proven performance as reported in peer-reviewed literature Applications: Successful, robust and high fidelity PCR regardless of conditions Amplification of products up to 30 kb (human genomic DNA), 40 kb (lambda DNA) or 13.5 kb (human cDNA) Easy amplification of GC- or AT-rich templates and targets containing repeats High tolerance for GC-rich template, excess nucleic acid, and when amplifying long products