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Amino acid analysis using Andrew+ Automated Preparation

Waters
6 Dec 2023

Amino acids are the most basic components that make up proteins, making them essential components of cell culture media and food stuffs. Monitoring and optimizing amino acid components of bioreactor media is essential for ensuring the best growing conditions for the cells. Likewise, it is necessary to confirm that food products meet specified requirements. The preparation and analysis of samples is a time-consuming process. Automated laboratory preparation systems provide the flexibility of freeing analysts' time for other tasks, resulting in a more efficient way of time management.

In this application note, Waters Corporation presents automated sample preparation protocols for the Andrew Alliance Andrew+ platform in conjunction with the AccQ●Tag Ultra Derivatization Automation Kit and amino acid standard kits. The AccQ●Tag Ultra Derivatization Kit for automation scales up reagent volumes necessary for use with automation systems due to their increased dead volume requirements. The reagent volumes provided allow for the preparation of up to 96 samples in a 3 x 32 sample format.

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FiltrationFiltration and microfiltration are used to separate solids, particulates or large molecules from fluids (liquid or gases) in order to purify the filtrate. Explore a range of filter formats, including filter paper, plates and membranes for your separation needs. While larger particulates may be separated by gravity filtration, smaller particulates may require additional force for timely separation. Microfiltration equipment includes vacuum filters, as well as positive pressure, crossflow and centrifugal filtration devices. Find the best filtration products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Sample ManagementSample management systems include sample storage devices such as freezers and plate storers, sample environment enclosures and sample organization, retrieval and sorter systems. Useful system features include high-throughput, automation, robotic arms, automated liquid handling and associated database systems. Accessories in sample management include barcode scanners, heat sealers and tubes.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Sample PreparationSample preparation can improve the quality and speed of separation techniques. Products to assist sample preparation include filtration equipment, evaporators, membranes and sieves.Amino AcidsCell Based AssaysCell-based assays use living cells to measure biological responses to various treatments or stimuli. They are essential for drug discovery, toxicology testing, and understanding cellular mechanisms. These assays are used to assess cell viability, proliferation, gene expression, and other cellular activities. Browse our peer-reviewed product directory to find the best cell-based assay tools and reagents, compare products, read customer reviews, and get pricing directly from manufacturers.Cell CultureCell culture involves growing cells under controlled conditions, typically outside of their natural environment, to study cellular behavior, drug response, and disease models. Cell culture is crucial in biotechnology and biomedical research. Explore cell culture products in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.

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Amino acid analysis using Andrew+ Automated Preparation