Accurate automated cell counting and viability analysis of trypan blue-stained PBMCs using the LUNA-III Cell Counter
28 May 2026This application note demonstrates how the LUNA-III™ Automated Cell Counter improves the accuracy of peripheral blood mononuclear cell (PBMC) counting and viability assessment using trypan blue staining. By combining an enhanced cell detection algorithm with focal plane offset adjustments, the system provides results comparable to fluorescence-based methods while maintaining a cost-effective brightfield workflow. Discover how the approach supports reliable measurement of cell concentration and viability across a range of sample conditions.
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