ResourceLife Sciences
ABEL® Cell Activation Assay for Whole Blood or Isolated Cells with Pholasin® and Adjuvant-K™
13 Jan 2015The activation of leukocytes present in the blood can be demonstrated through the luminescence produced by Pholasin® and Adjuvant-K™ in response to free radical production. This application note demonstrates the ability of the Orion II microplate luminometer to detect the production of reactive oxygen species produced by living cells from a small sample (20 μL) of capillary blood.
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Blood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.