ResourceLife Sciences
A workflow for membrane protein purification followed by detergent-free analysis
20 Nov 2023In this app note, Cytiva presents a workflow for membrane protein purification followed by detergent-free analysis. The traditional use of detergents for membrane solubilization is necessary for purification since membrane proteins are insoluble in aqueous solutions. The study's objective is to purify membrane proteins and subsequently reconstitute them into Salipro™ particles, where the proteins are surrounded by lipids instead of being in detergent micelles. The significance of this approach lies in its potential to enable Biacore™ analysis without the presence of detergents, allowing for the use of more native conditions in the analysis of membrane proteins.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein QuantificationThe detection and quantification of proteins in a sample is vital across life sciences, pharmaceutical research and clinical diagnostics, and a variety of equipment is available to scientists to simplify the workflow. Proteins of interest can be easily labeled and detected on light-based detection instruments. Immunoassay kits allow you to identify a specific protein of interest and protein detection beads or antibody microarrays allow you to identify multiple specific proteins at once. Protein interactions and enzyme activity can also be monitored with protein-protein interaction assays. Additionally, the biophysical characterization of proteins is made easy with biokinetic analyzers. Find the best protein detection and quantification products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Protein DetectionProtein detection techniques are essential for understanding protein expression, localization, and function. Methods such as western blotting, ELISA, and mass spectrometry are commonly used in research and diagnostics. Browse our peer-reviewed product directory to find the best tools for protein detection, compare products, check reviews, and get pricing directly from manufacturers.Membrane ProteinsLipidsLipids are a diverse group of organic compounds that are insoluble in water, including fats, oils, and hormones. They are critical for cellular structure and energy storage. Explore lipid analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.