A Standardized, Automated Approach for Exosome Isolation and Characterization Using Beckman Coulter Instrumentation

This application note describes the use of automated Biomek methods for next generation sequencing on benign and cancerous colon cell lines. Exosomes are small microvesicles, derived from the late endosome, most often described in the literature to be less than 120 nm, released by all cell types, and proven to be involved in cancer metastasis. Exosomes contain proteins, lipids, and microRNA capable of regulating an assortment of target genes. The results of this experiment demonstrate the capabilities of Beckman Coulter instrumentation in producing high impact next generation sequencing data with only a single MiSeq sequencing run. This method can be used to identify potential biomarkers and measure differential expression against sample type.