A New Tool for Biomarker Discovery
17 Dec 2013This article describes a new PEP technology, combining 2D-gel and mass spectrometry, intended to fill in the proteome knowledge gap. It provides a tool to systematically analyze the function of the proteins from a proteome and creating an overall picture of how the proteins are working as a system in important diseases.
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PEP Universal Protein Purification Kit
Array Bridge Inc.Complete kit for the fast purification of any functional protein from a protein mixture or a proteome with hundreds to thousands of proteins. It can also be used to build the three-dimensional enzyme landscape for any protein family for which a functional assay is available such as Protein Kinases, Protein Photosphatases, Proteinases, Oxido-Reductases etc.In the PEP technology, protein mixture is first separated by a modified one-dimensional or two-dimensional gel electrophoresis, this modified procedure provides good resolution whilte still maintaining protein function. This is followed b an efficient protein transfer step to a specially designed 384-well Protein Elution Plate (PEP).It is also possible to analyze multiple enzyme families in parallel to obtain functional profiles for each enzyme. The PEP technology can be applied to the systematic analysis of any proteins for which functional assays exist.