ResourceLife Sciences
A GloMax Multi Microplate Fluorometer Method for Fluorescein Measurement
10 May 2015Fluorescein is a commonly used fluorophore in many research applications such as microscopy, forensics, and serology to detect latent blood stains, immunoreagent, and hybridization probe labeling, as well as reference standards for instrument calibration. This application note describes how the GloMax® Multi Microplate Fluorometer can detect as little as 2.5 pM of fluorescein, with a linear dynamic range close to six decades.
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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.FluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.