Gene Expression and Molecular Cloning Products & Reviews

Easy to use, scalable enzymatic method for removing double-stranded RNA (dsRNA) from RNA samples. Remove dsRNA without the need for chromatographic methods like HPLC. No reduction in single-stranded RNA (ssRNA) yields. Reduces the innate immunogenic response to mRNA in cells.

Inhibits eukaryotic RNases A, B and C. Use as a safeguard against RNA degradation in any RNA-containing reaction.

Optimized for high-yield synthesis of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 100 µg of Ψ-RNA from 1 µg of template in 2 hours. Induce lower innate immune responses in mammalian cells by using Ψ-mRNA.

All-in-one kit to produce N1-methyl-pseudouridine-containing-mRNA (N1meΨ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of N1meΨ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using N1meΨ-mRNAs.

Specially formulated for maximum yields of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 160 µg of Ψ-RNA from 1 µg of template in 1 hour. Easily scale up to produce milligram amounts of Ψ-RNA. Induce lower innate immune responses in mammalian cells by using Ψ-mRNAs.

Optimized for high-yield synthesis of pseudouridine-containing, in vitro transcribed RNA (Ψ-RNA). Produce up to 60 µg of Ψ-RNA from 1 µg of template in 2 hours. Easily scale up to produce milligram amounts of Ψ-RNA. Induce lower innate immune responses in mammalian cells by using Ψ-mRNA.

For post-transcriptional poly(A) tail addition to RNAs. Tail mRNAs in vitro to lengths equivalent to or greater than those found in nature.

Co-transcriptionally cap 5-methyl-cytidine- pseudouridine-containing, in vitro transcribed RNA with Anti-Reverse cap analog (ARCA). Produce up to 40 µg of capped 5mCΨ-RNA from 1 µg of template in 3 hours. Optimized for maximum transcript capping and yield. Induce lower innate immune responses in mammalian cells by using 5mCΨ-mRNAs.

Quantitate mRNA capping efficiency (percent capped RNA content) using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective, benchtop method useful for QC as an alternative to HPLC and LC-MS analysis.

Quantitate the percentage of mRNA with Cap 1 vs Cap 0 5’-cap structures using standard polyacrylamide gel electrophoresis (PAGE). Easy, cost-effective benchtop method for QC as an alternative to mass spec analysis.

All-in-one kit to produce pseudouridine-containing-mRNA (Ψ-mRNA). Combine the benefits of high-yield transcription with post-transcriptional capping and tailing. Produce large amounts of Ψ-mRNA with virtually 100% transcript capping. Induce lower innate immune response in mammalian cells by using Ψ-mRNAs.

Specially formulated for maximum yields of N1-methyl-pseudouridine-containing, in vitro transcribed RNA (N1meΨ-RNA). Produce up to 160 µg of N1meΨ-RNA from 1 µg of template in 1 hour. Easily scale up reactions to produce milligram amounts of N1meΨ-RNA. Induce lower innate immune responses in mammalian cells by using N1meΨ-mRNAs.

Completely hands-off, walk-away HCP analysis in 1 hour.

The SpectraMax® DuoLuc™ Reporter Assay Kit enables highly sensitive quantitation of both firefly and Renilla luciferases in mammalian cells at a great price. Flash-type luminescence with excellent sensitivity Expression measurement in as few as ten cells per well Improved accuracy of results by normalizing reporter expression to a control Optimized for SpectraMax® i3x, iD3, or L microplate readers with injectors…

Gene Pulser®/Micropulser™ electroporation cuvettes are high-quality electroporation cuvettes which enable reproducible and consistent gene delivery to valuable transfection samples.

Pkg of 100, bursts at 1,800 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

Pkg of 100, bursts at 1,550 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

Pkg of 100, bursts at 1,100 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

Pkg of 100, bursts at 900 psi in a partial vacuum, for use with the PDS-1000/He biolistic system (#165-2257, #165-2258)

High-throughput sialylation screening for expediting the clone selection process

Create your own custom microarray using eArray, Agilent's online design application, and focus on the targets of interest for your specific research needs.

The Agilent SurePrint G3 Human Exon Microarrays include probes targeting individual exons, making it possible to look beyond the gene to more subtle but relevant expression changes at the exon level. Agilent Exon Microarrays are the fast, simple, and cost-effective choice for exon expression analysis, with capabilities to drill down into important RNA changes at the exon level.

Agilent’s Low Input Quick Amp WT Labeling Kit reliably amplifies and labels whole transcripts for eukaryote and prokaryote samples, with a low sample input range of 25-100 ng total RNA.

Agilent Gene Expression Microarrays provide a comprehensive coverage of genes and transcripts to study gene expression profiling for a wide variety of organisms and species.

The Agilent SurePrint G3 Mouse Exon Microarrays include probes targeting individual exons, making it possible to look beyond the gene to more subtle but relevant expression changes at the exon level. Agilent Exon Microarrays are the fast, simple, and cost-effective choice for exon expression analysis, with capabilities to drill down into important RNA changes at the exon level.