Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
DyNAzyme™ EXT DNA Polymerase is an optimized mixture of DyNAzyme™ II DNA Polymerase and a proofreading enzyme. It is a versatile and easy-to-use enzyme especially suited for handling demanding reaction conditions without time-consuming optimization.DyNAzyme™ EXT PCR Kit is designed to provide superior PCR amplifications for 100 long and difficult PCR or 200 standard amplifications. The kit also contains control template and tw…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7 when using Phusion™ HF Buffer) and speed previously unattainable with a single enzyme, even on your most difficult templates. Phusion™ High-Fidelity PCR Master Mix with Phusion™ HF Buffer is a convenient 2x mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer (2x Phusion HF Buffer) including MgCl2 for 100 reactions in 50 μl…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7 when using Phusion™ HF Buffer) and speed previously unattainable with a single enzyme, even on your most difficult templates. However, Phusion™ GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, i.e. GC-rich templates or those with complex secondary structures. Use of the Phusion GC Buffer is recommend…
RobusT™ I RT-PCR Kit is a high-fidelity one-tube system that is designed for convenient, sensitive and reproducible amplification of a specific target RNA up to 6.0 kb in length. The performance and excellent thermostability of the RobusT I RT-PCR Kit is based on AMV Reverse Transcriptase (AMV RT) in the first strand cDNA synthesis and on DyNAzyme™ EXT DNA Polymerase in the second strand synthesis and subsequent DNA amplificat…
RobusT™ II RT-PCR Kit is a high-fidelity one-tube system that is designed for convenient, sensitive and reproducible amplification of a specific target RNA up to 6.0 kb in length. The performance and excellent thermostability of the RobusT II RT-PCR Kit is based on M-MuLV Reverse Transcriptase RNase H- in the first strand cDNA synthesis and on DyNAzyme™ EXT DNA Polymerase in the second strand synthesis and subsequent DNA ampli…
DyNAmo™ SYBR® Green qPCR Kit is a ready-to-use 2x master mix for sensitive, quantitative real-time PCR. Only template DNA and PCR primers need to be added by the user. DyNAmo™ DNA Polymerase (modified Thermus brockianus DNA polymerase) in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. The kit is compatible with block-based real-time qPCR instruments that do…
DyNAmo™ SYBR® Green qPCR Kit with ROX™ passive reference dye is a ready-to-use 2x master mix for sensitive, quantitative real-time PCR. The ROX™ passive reference dye is supplied with the kit in a separate tube. Only template DNA and PCR primers need to be added by the user. DyNAmo™ DNA Polymerase (modified Thermus brockianus DNA polymerase) in this kit incorporates a non-specific DNA-binding domain that lends physical stabili…
DyNAmo™ HS SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. The kit is compatible with all block-based real-time qPCR instruments. ROX™ passive reference dy…
DyNAmo™ Capillary SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR with instruments using capillary tubes. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.
DyNAmo™ SYBR® Green 2-step qRT-PCR Kit enables reproducible detection and quantification of RNA with great sensitivity and specificity. The kit contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kit. Alternatively, the user can use gene specific primers. The reverse transcriptase in the kit is M-MuLV RNase H+, which p…
DyNAmo™ Capillary SYBR® Green 2-step qRT-PCR Kit enables reproducible detection and quantification of RNA with great sensitivity and specificity with instruments using capillary tubes. The kit contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kit. Alternatively, the user can use gene specific primers. The reverse tra…
Finnzymes’ Phire™ Hot Start DNA Polymerase is a novel PCR enzyme. The polymerase incorporates a specific DNA-binding domain which shortens extension times, improves yields, and increases fidelity 2-fold compared to Taq polymerase. In addition, it our unique hot start technology allows complete reactivation of Phire DNA Polymerase in “zero-time” at standard cycling temperatures. This combination of features makes this polymeras…
Finnzymes' DyNAmo™ Flash Probe qPCR Kit allows enhanced performance for probe-based qPCR. Optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillar…
Thermo Scientific ABgene PCR tubes feature uniform, thin walls that give optimal and consistent heat transfer, reducing variation between samples. The low binding interior surface prevents reaction components from binding to side walls, ensuring maximum yield. The tight sealing cap minimizes evaporation and maintains optimal reaction concentration. Features Compatible with 0.5ml thermal cycler blocks. Easy-to-use 'snap s…
The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increas…
Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.…
Thermo Scientific Verso 1-step RT-qPCR Kits for probe chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I treatment…
Thermo Scientific Verso 1-step RT-qPCR Kits for SYBR Green chemistry offer a quick and simple way to detect mRNA. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. An optional RT Enhancer is also provided and can be used during the RT step to remove any DNA contamination, eliminating the need for DNase I trea…
Thermo-Start Taq DNA Polymerase is a hot-start enzyme combined with an optimized buffer system that gives superior specificity and sensitivity. The chemically modified Thermo-Start Taq remains completely inactive until it is switched on by a high temperature incubation step at the start of the PCR reaction. This prevents nonspecific amplification during reaction setup, eliminating smearing and the formation of nontarget bands.…
Thermo Scientific Verso 1-Step RT-PCR system is a complete set of RT-PCR reagents that combines everything needed for RT-PCR. The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures. Verso also shows a better dynamic range than standard AMV or M-MuLV enzymes, with increases in template concentration giving rise to c…
Thermo Scientific ABsolute QPCR Seal has been designed for QPCR applications. Features:• Optically clear for QPCR and other fluorescence applications • Strong pressure-sensitive adhesive prevents evaporation during thermal cycling or sample storage • Peelable • No sticky residue on the plate when the seal is peeled off • End tabs for easy seal application and removal Temperature range: -40ºC to +120ºC
The Thermo Scientific Omnistrips feature uniform, thin walls that give optimal and consistent heat transfer, reducing variation between samples. The low binding interior surface prevents reaction components from binding to side walls, ensuring maximum yield. The tight sealing strip caps significantly reduce evaporation to generate more efficient and higher yield results. Highlights • Suitable for 0.5 mL thermal cycler blocks •…
The Thermo Scientific 0.2 mL Strip Tubes with caps are available with flat, domed, or Ultra Clear caps. These strip tubes are designed for PCR and qPCR applications, and are suitable for 0.2 mL thermal cycler blocks and 96-well V-bottom thermal cyclers. The 8-strip tubes can be divided into individual tubes. Highlights • Suitable for 0.2 mL thermal cycler blocks • 0.25 mL maximum tube capacity when capped • 8 tube strips can b…
The Thermo Scientific 0.2 mL Strip Tubes come in eight-tube strips and are compatible with standard 0.2 mL thermal cycler blocks. They are designed for both PCR and qPCR applications. While these tubes are sold without caps, Thermo Scientific offers multiple cap options, including an ultra clear cap that's ideal for use in qPCR assays. The caps form a secure seal, yet are easy to apply and remove. Caps increase the overall tub…
Domed and flat cap strips. Also ultra-clear caps for qPCR.
