Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
Phusion™ RT-PCR Kit is a complete two-step kit designed for high-fidelity RT-PCR. The kit contains the full set of reagents required for performing cDNA synthesis and PCR in two steps. In the first step, a variety of RNA templates and all priming options may be used for efficient synthesis of cDNA with M-MuLV Reverse Transcriptase.In the second step, Phusion Hot Start High-Fidelity DNA Polymerase is used for cDNA amplification…
Phusion™ Flash High-Fidelity PCR Master Mix is a 2x master mix enabling the use of extremely short PCR protocols without compromising neither the fidelity nor the yield in the reaction. Phusion Flash DNA Polymerase used in the master mix is a proofreading DNA polymerase modified from Phusion Hot Start High-Fidelity DNA Polymerase. Phusion Flash High-Fidelity PCR Master Mix enables the use of extremely short cycling protocols w…
Finnzymes’ DyNAmo™ Flash SYBR® Green qPCR Kit is developed for fast real-time qPCR. It provides qPCR results faster than other SYBR Green kits without compromising the qPCR performance.A DNA-binding domain attached to the polymerase in this kit improves the physical stability of the polymerase-DNA complex, thus enhancing the processivity and robustness of the amplification. The high amplification efficiency of DyNAmo Flash SYB…
The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications. This kit can be used in conjunction with Finnzymes' DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed…
The Phusion™ Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with Quick T4 DNA Ligase. The resulting…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7) and speed previously unattainable with a single enzyme, even on your most difficult templates. Phusion™ High-Fidelity PCR Kit contains all the necessary reagents for performing 50 amplifications in 50 μl volume or 125 amplifications in 20 μl volume. The kit also contains control template (λ DNA) and two primer sets (1.3 kb and 10 kb) for performing 2…
Finnzymes' DyNAzyme™ II Hot Start DNA Polymerase is a chemically modified hot start form of the DyNAzyme™ II DNA Polymerase originating from Thermus brockianus. DyNAzyme II Hot Start DNA Polymerase is inactive at room temperature, and the activity is recovered by a 10-minute incubation step at 94 °C.The enzyme improves the specificity of PCR by preventing the extension of non-specifically bound primers during reaction setup an…
DyNAzyme™ II PCR Master Mix is available as a 2x mix containing recombinant DyNAzyme II DNA Polymerase (isolated and purified from E.coli strain carrying a plasmid with the cloned DyNAzyme™ DNA Polymerase gene from Thermus brockianus), nucleotides and optimized reaction buffer including MgCl2. Only template and primers need to be added by the user.
DyNAzyme™ I DNA Polymerase Kit contains DyNAzyme™ I DNA Polymerase, buffers, ready-to-use DNA size standard (λ DNA Hind III and øX174 DNA Hae III digest) and nucleotides for 100 amplifications in 50 μl volume. The kit also contains control template and primers for 40 control reactions.
DyNAzyme™ EXT DNA Polymerase is an optimized mixture of DyNAzyme™ II DNA Polymerase and a proofreading enzyme. It is a versatile and easy-to-use enzyme especially suited for handling demanding reaction conditions without time-consuming optimization.DyNAzyme™ EXT PCR Kit is designed to provide superior PCR amplifications for 100 long and difficult PCR or 200 standard amplifications. The kit also contains control template and tw…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7 when using Phusion™ HF Buffer) and speed previously unattainable with a single enzyme, even on your most difficult templates. Phusion™ High-Fidelity PCR Master Mix with Phusion™ HF Buffer is a convenient 2x mix containing Phusion DNA Polymerase, nucleotides and optimized reaction buffer (2x Phusion HF Buffer) including MgCl2 for 100 reactions in 50 μl…
Phusion™ High-Fidelity DNA Polymerase offers accuracy (error rate 4.4 x 10-7 when using Phusion™ HF Buffer) and speed previously unattainable with a single enzyme, even on your most difficult templates. However, Phusion™ GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, i.e. GC-rich templates or those with complex secondary structures. Use of the Phusion GC Buffer is recommend…
RobusT™ I RT-PCR Kit is a high-fidelity one-tube system that is designed for convenient, sensitive and reproducible amplification of a specific target RNA up to 6.0 kb in length. The performance and excellent thermostability of the RobusT I RT-PCR Kit is based on AMV Reverse Transcriptase (AMV RT) in the first strand cDNA synthesis and on DyNAzyme™ EXT DNA Polymerase in the second strand synthesis and subsequent DNA amplificat…
RobusT™ II RT-PCR Kit is a high-fidelity one-tube system that is designed for convenient, sensitive and reproducible amplification of a specific target RNA up to 6.0 kb in length. The performance and excellent thermostability of the RobusT II RT-PCR Kit is based on M-MuLV Reverse Transcriptase RNase H- in the first strand cDNA synthesis and on DyNAzyme™ EXT DNA Polymerase in the second strand synthesis and subsequent DNA ampli…
DyNAmo™ SYBR® Green qPCR Kit is a ready-to-use 2x master mix for sensitive, quantitative real-time PCR. Only template DNA and PCR primers need to be added by the user. DyNAmo™ DNA Polymerase (modified Thermus brockianus DNA polymerase) in this kit incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. The kit is compatible with block-based real-time qPCR instruments that do…
DyNAmo™ SYBR® Green qPCR Kit with ROX™ passive reference dye is a ready-to-use 2x master mix for sensitive, quantitative real-time PCR. The ROX™ passive reference dye is supplied with the kit in a separate tube. Only template DNA and PCR primers need to be added by the user. DyNAmo™ DNA Polymerase (modified Thermus brockianus DNA polymerase) in this kit incorporates a non-specific DNA-binding domain that lends physical stabili…
DyNAmo™ HS SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex. The kit is compatible with all block-based real-time qPCR instruments. ROX™ passive reference dy…
DyNAmo™ Capillary SYBR® Green qPCR Kit is a ready-to-use 2x master mix for highly specific quantitative PCR with instruments using capillary tubes. The 2x master mix includes a hot start version of a modified Thermus brockianus DNA polymerase. The modified DNA polymerase incorporates a non-specific DNA-binding domain that lends physical stability to the polymerase-DNA complex.
DyNAmo™ SYBR® Green 2-step qRT-PCR Kit enables reproducible detection and quantification of RNA with great sensitivity and specificity. The kit contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kit. Alternatively, the user can use gene specific primers. The reverse transcriptase in the kit is M-MuLV RNase H+, which p…
DyNAmo™ Capillary SYBR® Green 2-step qRT-PCR Kit enables reproducible detection and quantification of RNA with great sensitivity and specificity with instruments using capillary tubes. The kit contain all necessary reagents for cDNA synthesis from various RNA sources and for subsequent qPCR. Both random primers and oligo(dT) primers are included in the kit. Alternatively, the user can use gene specific primers. The reverse tra…
Finnzymes’ Phire™ Hot Start DNA Polymerase is a novel PCR enzyme. The polymerase incorporates a specific DNA-binding domain which shortens extension times, improves yields, and increases fidelity 2-fold compared to Taq polymerase. In addition, it our unique hot start technology allows complete reactivation of Phire DNA Polymerase in “zero-time” at standard cycling temperatures. This combination of features makes this polymeras…
Finnzymes' DyNAmo™ Flash Probe qPCR Kit allows enhanced performance for probe-based qPCR. Optimized reagents and extremely short cycling times deliver maximum speed thus increasing sample throughput. Significant time savings are achieved with both conventional and fast real-time PCR instruments. DyNAmo Flash Probe qPCR Kit is optimized for hydrolysis probes (e.g., TaqMan probes) and it can be used with both block- and capillar…
Thermo Scientific ABgene PCR tubes feature uniform, thin walls that give optimal and consistent heat transfer, reducing variation between samples. The low binding interior surface prevents reaction components from binding to side walls, ensuring maximum yield. The tight sealing cap minimizes evaporation and maintains optimal reaction concentration. Features Compatible with 0.5ml thermal cycler blocks. Easy-to-use 'snap s…
The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increas…
Thermo Scientific ABsolute Blue qPCR Mixes are 2X master mixes for probe chemistry. There are suitable alternatives available for all common real-time instruments. ABsolute Blue qPCR Mixes utilize Thermo-Start Taq hot start enzyme for increased sensitivity. The master mixes incorporate an inert blue dye to enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.…
