PCR Mastermixes and Kits Products & Reviews

Lentiviruses are commonly used as viral vectors in cell and gene therapy production, delivering and steadily integrating desired genes into the genome of the host cell. However, they may also present health hazards to humans due to integration-mediated transformation and the creation of a RCL that can infect nontarget cells. While the current regulatory guidelines recommend a cell culture–based method for RCL testing, it is cu…

Adeno-associated virus (AAV) is commonly used as a viral vector in cell and gene therapy production, but there is a risk of it becoming a replication competent virus (RCV) during the production process, especially in the presence of a helper virus, which can raise safety concerns. While the guidelines recommend a cell culture–based method for RCAAV testing, it is cumbersome and takes 30–45 days from sample to results. Verichec…

The Agilent range of HotStart polymerases and master mixes features Hotstart Taq DNA Polymerase, Methylation Bi-sulfite sequencing, uracil-sensitive solutions and more. 

First generation Pfu polymerases, such as cloned Pfu, PfuTurbo, and PfuUltra, have been improved upon. The improved products, PfuUltra II and Herculase II provide all the benefits of the first generation enzymes plus additional features such as speed to results and larger amplicons.  Cloned Pfu DNA Polymerases  First generation     PfuUltra    DNA Polymerases     PfuTurbo    DNA Polymerases

The PicoMaxx high fidelity PCR system reliably produces high PCR product yields on a wide variety of templates up to 10 kb. It also provides superior sensitivity by successfully amplifying samples where starting material is limited.  High sensitivity provides maximum PCR reliability  Consistent, robust amplification up to 10 kb  Superior efficiency and yield with our patented ArchaeMaxx polymerase-enhancing factor  Opti…

The PicoMaxx high fidelity PCR system reliably produces high PCR product yields on a wide variety of templates up to 10 kb. It also provides superior sensitivity by successfully amplifying samples where starting material is limited.  High sensitivity provides maximum PCR reliability  Consistent, robust amplification up to 10 kb  Superior efficiency and yield with our patented ArchaeMaxx polymerase-enhancing factor  Opti…

PicoMaxx High Fidelity PCR Master Mix possesses the sensitivity to detect low copy number targets. Plus, it has the robustness to withstand effects of PCR inhibitors without the need for extensive purification and subsequent DNA loss. PicoMaxx reliably produces high PCR product yields on a wide variety of templates up to 10 Kb.

The Paq5000 DNA polymerase is a robust Taq substitute that is derived from a Pyrococcus species. This enzyme provides equivalent and often better performance to endpoint Taq DNA polymerase amplification and can be substituted easily into many Taq-based procedures.  Robust, cost-effective alternative to Taq  Equal to better yield with faster cycling conditions  Amplify up to 6 kb genomic DNA targets  Easily substituted…

The TaqPlus Precision polymerase mixture is an optimized blend of Stratagene cloned Pfu DNA polymerase and Taq2000 DNA polymerase, a highly purified, recombinant version of Taq DNA polymerase. The TaqPlus Precision PCR system exhibits the highest replication accuracy achieved using DNA polymerase mixtures.

Easy-A Hi-Fi Cloning Enzyme & Master Mix combines the higher accuracy of a proofreading enzyme with the high cloning efficiency of Taq. PCR products amplified with the Easy-A can be cloned directly, without performing additional steps typically required when amplifying with proofreading polymerases.

Taq Extender PCR additive improves the reliability and yield of conventional Taq-based PCR amplifications. Taq Extender additive increases the efficiency of Taq DNA polymerase extension reactions during each cycle of PCR by reducing mismatch pausing, resulting in a greater percentage of completed extension reactions.

RayBiotech Monkeypox Virus (MPXV) PCR Nucleic Acid Detection Kit is a real-time PCR assay for the detection of MPXV DNA in a liquid sample. It uses a fluorescent probe with specific primer sets to detect the J2R gene specific for the genome of MPXV.

DNA-free Taq DNA polymerases for contamination-free analysis

Universal 16S rDNA real-time PCR assay for sensitive detection of bacterial DNA

Ultra-clean PCR reagents for use with custom primers

Universal 18S rDNA real-time PCR assay for sensitive detection of fungal DNA

The PowerPlex® Y23 System is a 23-loci, 5-color Y-STR multiplex designed for genotyping forensic casework samples, database samples and paternity samples.   The kit contains: all 12 loci in the current PowerPlex® Y System, the additional 5 loci found in AmpFlSTR® Y-filer®, plus 6 new loci.   PowerPlex® Y23 System Benefits: Reveal more Y-STR loci in excess female DNA Cut your amplification time roughly in ha…

The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase.   Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…

GoTaq® Long PCR Master Mix contains the high-performance GoTaq® Hot Start Polyemrase in a specially formulated mixture with a proprietary thermostable proofreading polyemrase.   This optimized enzyme mixture allows efficient amplification of up to 40kb from lambda DNA or 30kb from human genomic DNA. The presence of a proofreading enzyme to repair DNA mismatches and a highly processive polyemrase allows the polyemras…

Preformulated PCR Master Mix Components to Determine Optimal PCR Amplification Conditions

Standardized Screening Panel Amplify key functional regions associated w/Azoospermia Factor (AZF)

Inhibits Polymerase Activity at Temperatures below 70°C High conc and glycerol-free formulations

Inhibits Polymerase Activity at Temperatures below 70°C High conc and glycerol-free formulations

Inhibits Polymerase Activity at Temperatures below 70°C High conc and glycerol-free formulations

Confidence for Mixed Sample Analysis Track and confirm tissue specimen provenance and cell lineage