Gene Expression and Molecular Cloning Products & Reviews

Real-time fluorescent DNA detection

The gold standard in bead-based NGS cleanup and size selection

The DNA Ligation Kit Mighty Mix is a single solution, ready-to-use 2X ligation mix that enables high efficiency ligations, particularly for blunt-ended ligations. This DNA ligation kit can also be used for ligation into TA-vectors. The basic ligation reaction can be quickly performed, either for 5 minutes at 25°C or for 30 minutes at 16°C, with equal results for both circular and linear DNA ligations. The 2X Ligation Mix is c…

Roche FuGENE HD Transfection Reagent is a next-generation transfection reagent, free of animal-derived components. Roche FuGENE HD Transfection Reagent is designed to transfect a wide range of eukaryotic cells, including insect cells and many tumor cell lines not transfected well by other reagents (e.g., MCF7, Hep G2, PC-3, HeLa, MA-10, Hep G2, SH-SY5Y, A7r5, STO, SCC-61, SQ20B, T98G, STSAR-90, A375, A549, and others). In addi…

The Nucleofector technology is a highly efficient non-viral method for transfection. It is based on two components: the Nucleofector Device that delivers unique electrical parameters, and Nucleofector Kits that contain cell-type-specific Nucleofector Solutions. The electrical parameters of the Nucleofector are different from any other commercially available electroporation device. Each electrical setting is displayed as a dist…

The New Standard for Desktop-Based Bioinformatics Tools CLC DNA Workbench provides a software environment which enables users to perform advanced DNA sequence analyses such as assembly of DNA sequence data, graphically and algorithmically advanced primer design, while offering user-friendly molecular cloning tools. CLC DNA Workbench is a bioinformatics software package containing a range of specialized DNA analyses and bioinfo…

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. Features: High yields of capped RNA—up to 39 µg of RNA per reaction  Extra convenience—cap analog-nucleotide mix provided  Simplified rea…

Introducing the versatile reagent with the power to transfect your most difficult cells.

Contains one vial of Lipofectamine® 3000 Reagent (1.5 mL) and one vial of P3000™ Enhancer Reagent.

Ready-to-use, easy interpretation of results, Highly-sensitive, no need for internal control

Universal, clinically applicable enhancer solution for lentivirus based transductions.

C. elegans transgenics built to your specs

Let The Experts Get The Work Done

Transform into E. coli without clean-up or desalting

Lentivirus Gene Expression Vector

SEQuoia Express Stranded RNA Library Prep Kit enables customers to overcome the challenge of a laborious library prep workflow by providing a cost-effective 3-hour, 3-tube, automation-friendly solution for constructing robust libraries that efficiently capture long RNA transcripts (>200bp).

Versatile — works with a broad range of cell lines Reproducible — produces consistent results, lot-to-lot, plate-to-plate, and well-to-well Efficient — performs with high siRNA transfection efficiency, allowing use of low siRNA concentrations to minimize nonspecific effects Fast — reverse-transfects cells as they are plated, saving a day

Roche FuGENE 6 Transfection Reagent is a non-liposomal multicomponent reagent well-suited for experiments involving cellular analysis. Due to the simple methodology of FuGENE 6 Transfection Reagent, minimal optimization, low cytotoxicity, and ability to provide high transfection efficiency even in the presence of serum, it is one of the standard transfection reagents in many different laboratories around the world. With a ment…

High Capacity RNA-to-cDNA Kit is a streamlined reverse transcription kit designed for optimum performance with the TaqMan® Gene Expression Master Mix, Power SYBR® Green PCR Master Mix and others. The components of the two-tube kit work together to provide sensitive and specific reverse transcription across a broad range of template quantities. The new High Capacity RNA-to-cDNA formulation reduces reverse transcription time by…

TaqMan®  MicroRNA Arrays provide all the advantages of TaqMan®  MicroRNA Assays in a convenient, pre-configured micro fluidic card minimizing experimental variability and effort required to run 384 TaqMan MicroRNA Assays in parallel. Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and simplify the TaqMan MicroRNA Array. When sensitivity is of th…

Roche NimbleGen provides a unique combination of high-density arrays, long-oligo probes, and flexible design capability that together provide superior results for advanced gene expression analysis. NimbleGen microarrays enable accurate, sensitive, and specific interrogation of genome-wide expression for any sequenced and annotated genome. Researchers also can tile through a genome at any desired spacing to discover genome-wide…

Key Features of the cloning technology Clone any insert, at any site within any vector Restriction enzyme, phosphatase and ligase free system Powerful technology to join multiple fragments at once Rapid one reaction for 5 minutes at room temperature, then fusion on ice for 10 minutes Introduce or correct mutations at will in cDNAs Amenable to high throughput cloning applications

Exiqon miRCURY LNA™ First-strand cDNA Kit First-strand cDNA synthesis reagents for high performance microRNA real-time PCR using the miRCURY LNA™ microRNA PCR system. Features of the Exiqon miRCURY LNA™ First-strand cDNA Kit: • Optimized for fast, easy and reliable first-strand cDNA synthesis using our miRCURY LNA™ microRNA PCR system • Includes a thermostable and highly reliable reverse transcriptase The miRCURY LNA™ Fir…

Features of the Drummond Scientific Nanoject II Auto-Nanoliter Injector include: • Automated Microprocessor controlled Microinjection Pipet delivering precise nanoliter volumes accurately • Remote control and nonrotating plunger eliminates vibration allowing precise injection minimizing possible cell rupture from microinjector• Selectable Injection Volumes: from 2.3 nanoliters to 69.0 nanoliters in 16 increments • Supplied wi…

Developed through years of experience with the Flaming/Brown style micropipette pullers and infused with leading edge technology, Sutter is proud to introduce the P-1000 micropipette puller. The most obvious new feature is the color touchscreen display that provides an intuitive and full-featured interface. Key Features: Color touch-screen interface Safe heat mode to protect and extend filament life Line repeat mode sim…