Competent Cells Products & Reviews

A collection of classic strains and competent cells.

The ABLE C strain and the ABLE K strain help solve issues associated with toxic clones. These strains reduce the copy number of common cloning vectors by ~4- and 10-fold, enhancing the probability that a toxic clone will be propagated.

The ABLE C strain and the ABLE K strain help solve issues associated with toxic clones. These strains reduce the copy number of common cloning vectors by ~4- and 10-fold, enhancing the probability that a toxic clone will be propagated.

The most common method for transformation of plasmids into E. coli is to use chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility and convenience are achieved using prepared competent cells. Novagen offers the widest selection of competent cells available for protein expression. Our strains offer a variety of options that enable you to maximize the yield an…

High efficiency electroporation using any instrumentIngenio® Electroporation Kits and Solution provide a universal, high efficiency, low toxicity solution for electroporation of DNA or siRNA into hard to transfect cell types. Electroporation using Ingenio Electroporation Kits and Solution affords increased gene expression in several different cell types with minimal toxicity.Ingenio® Electroporation Solution Features:•High eff…

The Single Step (KRX) Competent Cells are designed for efficient transformation and tightly controlled protein expression.   These cells consolidate the best attributes of these two steps into one strain to evaluate protein expression in E. coli. Transformation efficiencies are greater than 108 cfu/μg, similar to other highly competent cells. The single step cells are available in single transformation size (50μl). KRX a…

For standard cloning experiments competent cells are available in two formats: high efficiency at greater than 108cfu/μg for cloning (JM109, HB101), and subcloning efficiency at greater than 107cfu/μg (HB101). JM109 cells have the additional feature of blue/white screening for recombinants. For protein expression, JM109(DE3), BL21(DE3) or KRX competent cells can be used. The Single Step (KRX) Competent Cells are designed fo…

Simple, fast, and controlled autolysis of E. coli.

Simple, fast, and controlled autolysis of E. coli.

Vmax™ X2 competent expression cells are a fast-growing bacterial strain designed and optimized for high-level recombinant protein expression. With a doubling time twice that of E. coli and the ability to generate greater amounts of biomass per volume of cells, Vmax™ X2 yields higher amounts of soluble protein in less time.