ECM675 uses purified chicken type I collagen in 6mM acetate buffer pH 7.0. The product is 99.9% pure native atelomeric avian collagen. Approximately 85% type I and 15% type III collagen protein. The material has been specially prepared to maintain its native state as much as possible. The telomeric ends of the collagen I have been enzymatically removed. Since most of the species identity is found within the telomeric ends, our…
The Multidrug Resistance Direct Dye Efflux Assay Kit includes two of the best characterized and most commonly used multidrug resistance ABC transporter substrates, DiOC2(3) and rhodamine 123. The kit enables researchers to directly assess the functional activity of the MDR1, MRP1 and BCRP membrane pumps in living cells under physiologic conditions by directly measuring the relative fluorescence of cell populations that activel…
CHEMICON's Actin Cytoskeleton and Focal Adhesion Staining Kit is a very sensitive immunocytochemical tool that contains fluorescent-labeled phalloidin (TRITC-conjugated phalloidin) to map the local orientation of actin filaments within cell and a mouse monoclonal antibody to Vinculin that is very specific for the staining of focal contacts in cells.
Chemicon's Mast Cell Degranulation Assay Kit provides a simple and convenient means for assaying tryptase activity. The assay is based on spectophotometric detection of the chromophore p-nitroaniline (pNA) after cleavage from the labeled substrate tosyl-gly-pro-lys-pNA. The free pNA can then be quantified using a spectrophotometer or a microtiter plate reader at 405 nm. A pNA Standard is provided as a comparison control.
CHEMICON's NS220 Neurite Outgrowth Assay Kit (3 µm) utilizes microporous tissue culture insert technology from EMD Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 3-μm pores at the base. The Millicell inserts utilized in this kit are appropriate for cells projecting neurites of up to 3 µm diameter, e.g. N1E-115 cells [Abe et al., 2003], Dorsal Root Ganglia and…
CHEMICON's NS225 Neurite Outgrowth Assay Kit (1 μm) utilizes microporous tissue culture insert technology from EMD Millipore and is based on the use of Millicell cell culture inserts (chambers) containing a permeable membrane with 1 μm pores at the base. With this system, it is possible to screen numerous biological and pharmacological agents simultaneously, directly evaluate adhesion and guidance receptor functions responsibl…
ApopTag® Equilibration Buffer is intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
ApopTag® Terminal deoxynucleotidyl Transferase (TdT) is a recombinant enzyme intended for use in labeling the 3'-OH ends of fragmented DNA during apoptotic cell detection. Qualified for use with Apoptag® In Situ Apoptosis Detection Kits
Rat mammary gland undergoes programmed cell death processes during the tissue regression that follows lactation. Apoptotic bodies, which can be seen by routine histologic staining, can be specifically stained by techniques that localize DNA strand breaks in situ. The specificity of staining for strand breaks can thus be correlated with the morphological changes that define the process of apoptosis.
APOPTAG® Plastic Coverslips are designed to be custom fitted to the specific APOPTAG® kit application.
The ApopTag® Peroxidase In Situ Oligo Ligation Kit specifically detects apoptosis by staining only cells that contain double-stranded breaks that are blunt-ended or have a one base 3' overhang (cells containing nicked, gapped, 3'-recessed, 3'-overhanging ends longer than one base and single-stranded ends are not detected). The high analytic specificity of the ApopTag® ISOL Kit minimizes background due to non-specific labeling…
The TRAPeze® Telomerase Detection Kit is a highly sensitive in vitro assay system for detecting telomerase activity. The assay is a one-buffer, two-enzyme, system utilizing PCR to enhance the sensitivity of telomerase detection in small samples.
1X CHAPS Lysis Buffer is a component of the gel- and ELISA-based TRAPeze® Telomerase Detection Kits and is used in the extraction step of the procedure.
The TRAPEZE® XL Kit is a highly sensitive in vitro assay for the fluorometric detection of telomerase activity. As in the original TRAPEZE® Kit, primer sequence modifications that reduce amplification artifacts and an internal PCR control are included. In addition, the TRAPEZE® XL Kit uses fluorescence energy transfer (ET) primers to generate fluorescently labeled TRAP products which permit nonisotopic, quantitative analysis o…
The TRAPEZE® ELISA Kit provides the reagents necessary for performing the TRAP assay followed by ELISA detection of telomerase activity in cell/tissue samples.
The Amplifluor™ UniPrimer™-fluorescein is a hairpin primer labeled with a fluorescent energy transfer pair (fluorescein and non-fluorescent quencher) and a 3' single-stranded oligonucleotide tail and is provided at a concentration of 5.0 μM (25.0 mL).
The Amplifluor™ SNPs Genotyping System for Assay Development is designed for new users unfamiliar with the technology. This kit contains the fluorescein-, sulforhodamine- and JOE-labeled Amplifluor™ UniPrimers™ as well as control primers and templates.
The Amplifluor™ SNPs HT Genotyping System FAM-SR is designed for users familiar with Amplifluor™ and is for use with fluorescent plate readers.
The Amplifluor™ SNPs HT Genotyping System FAM-JOE is designed for users familiar with Amplifluor™ and is for users of real-time PCR detection instruments.
The Millicell μ-Angiogenesis activation and inhibition kits provide a powerful, quantitative platform for real-time monitoring of changes in tubule formation with unprecedented optical resolution.Continuous, live monitoring of cells as tubules formEven, flat slide surface prevents any liquid meniscus from forming, eliminating out-of-focus areasFull well visualization at low magnificationOptimized assay conditions in a miniatur…
3-channel SPR detection with Innovative multi-module design. Compatible with electrochemistry and chemical vapor applications.
Virtual tissues services for biomedical research features high fidelity tissue simulation and modelling to grow virtual tissue from a single cell, giving unprecedented insight into lineage commitment phenomena of stem cells with insight into signalling pathways and detailed gene regulatory networks.With Endogenics complementing traditional techniques, researchers can simulate human cell development and tissue organization and…
Commercially available antibodies often contain substances (e.g. BSA, glycine, tris, azide) that interfere in labeling reactions with enzymes or fluorophores. The AbSelect™ Purification Kit quickly removes these contaminants of antibody labelling. It can also be used to purify antibodies from crude samples such as ascites fluid or immune serum. The antibody to be purified or cleaned up ideally is in a volume of 100ul to 0.5m…
Say goodbye to secondary antibodies – Go direct using the World’s Easiest DIY Antibody Labeling Kit - Lightning-Link™! • Eliminates need for secondary antibodies • Reduces the number of wash steps • Ideal for multi-protein analysis – with over 40 available labels • Very easy to use • 100% antibody recovery Lightning-Link™ kits The DIY Lightning-Link™ kit allows you to label an antibody with minimal hands-on time - less than 30…
