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pBluescript II XR Library Construction Kit

Agilent Technologies

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.  New cDNA synt…

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LumiMax Superoxide Anion Detection Kit

Agilent Technologies

The LumiMax superoxide anion detection kit qualitatively detects the presence of superoxide anions and superoxide dismutase in cell cultures with a higher sensitivity than the standard luminol-based assay.  More sensitive than traditional assays (use as few as 10e4 cells)  Enhancer is non-cytotoxic and non-denaturing  Up to 40-fold signal enhancement for cultured cells  Proprietary enhancer which increases signal at lea…

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Mitochondrial Membrane Potential Detection Kit

Agilent Technologies

The Mitochondrial membrane potential detection kit uses a unique fluorescent cationic dye, JC-1, to signal the loss of mitochondrial membrane potential. In healthy non-apoptotic cells, the dye stains the mitochondria bright red.  Detect the loss in mitochondrial membrane potential - Early indicator of cellular apoptosis  Convenient kit format  Use with flow cytometer, fluorescence plate reader, fluorescence microscope

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pCMV-Script XR Library Construction Kit

Agilent Technologies

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.  CMV promoter for high-level protein expression in mammalian cells  Large multiple cloning site contains 15 unique sites  Clone PCR products generated with any PCR e…

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Yeast Plasmid DNA Isolation System

Agilent Technologies

The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD).  Detects protein-prote…

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In Situ beta-Galactosidase Staining Kit

Agilent Technologies

The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells.  In situ staining of cells directly in dishes without removing or manipulating cells  Rapid detection of Ÿ-gal activity in transfected cells  Results in 24 hours  Maximum signal with low background  Deep blue staining reaction  Kit in…

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Supercoiled DNA Ladder

Agilent Technologies

The Supercoiled DNA Ladder contains fragments from 2 kb to 10 kb at 1-kb increments and an additional band at 12 kb. Each supercoiled band is at a known concentration, with the 7-kb band at a higher concentration than the others. The resulting brighter band in the middle of the ladder acts as a useful reference point.  Accurate size determination facilitated by evenly spaced bands  Fragments are exact multiples of 1000 bp…

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