PCR and Thermal Cycling Products & Reviews

Positive snap-in transparent lid guarantees safe closure

The Roche Digital LightCycler® System is the digital PCR instrument of tomorrow. With a unique combination of 3 nanowell plate configurations, 6 advanced optical channels, and 5x concentrated DNA and RNA master mixes, it has the potential to help your lab to make the leap from publishing research to producing clinically viable assays.

A qPCR system designed to deliver accuracy and flexibility to research and clinical diagnostic laboratories. Leveraging the robustness and proven technology of the LightCycler® 4801, the LightCycler® PRO integrates innovative technology and Roche manufacturing to deliver accurate real-time PCR results, flexible solutions to your workflow, and an improved user experience to your lab.

The Micronic Eco-Friendly Choice Rack RM is 99% made of renewed material, setting a new sustainable standard for sample storage racks.

High-precision real-time fluorescence quantitative PCR system

The CpG WIZ® p16 Amplification Kit is used for determining the methylation status of the p16 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified such that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated)…

The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unm…

The CpG WIZ® E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and…

The CpG WIZ® Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also i…

CpGenome™ Universal Unmethylated DNA Set provides 2 separate genomic DNA controls for gene methylation studies. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) and can be subsequently used as an unmethylated DNA control for gene methylation studies.

The CpGenome™ DNA Modification Kit (Cat. No. S7820) contains the reagents necessary to perform the initial bisulfite reactions, while the CpG WIZ® BRCA1 Amplification Kit contains the reagents required for the PCR amplification reactions.

With a uniform thermal ramp rate of over 10°C per second xxpress™ is the fastest real-time thermocycler in the World delivering 40 cycle q-PCR in under 10 minutes. And whilst not all PCR chemistries are yet as fast as this system there are a number that repeatably and reliably work at this speed. Ultra low thermal mass and high conductively enable the patented resistive heating system deliver this speed cycle more efficiently…

An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…

A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…

Our uncloned normalized cDNA is created using proprietary technology which features full-length-enriched and high quality size-fractionated cDNA. Our cDNA has been used successfully with FLX, Titanium an Illumina sequencing.

Our cDNA libraries feature full-length-enriched and high quality size-fractionated cDNA. cDNA library construction is usually done in slightly-modified versions of pBluescript or pcDNA3.1. Insert sizes will range from about 0.5Kb to 4Kb. Concerning QC steps, we evaluate the average insert size, empty clone rate and clone concentration/tube before shipping out. We always provide a gel picture of randomly selected clones showing…

ASH is APA-based, full-length-enriched, single-stranded cDNA subtraction hybridization library. - ASH cDNA subtraction is full-length-enriched and independent of PCR suppression. - ASH cloning is directional and does not involve normalization. - ASH method provides non-exponential amplification before subtraction because double-stranded cDNA is not required. - ASH method has a better subtraction effect because 1) a high ratio…

RealTime ready Custom RT-qPCR Assays and Panels Get a head start on your gene expression analysis with function tested assays for all annotated human, mouse, and rat genes Achieve high specificity using intron-spanning assays based on short hydrolysis LNA-substituted Universal ProbeLibrary (UPL) Probes.  Rely on stringently RT-qPCR-tested assays that are compliant with MIQE (Minimum Information for Publication of Quantitati…

A Billion Reactions. Digital Answers. Experience the Next Generation of PCR. Capable of generating more than a billion reactions in a single day, the RainDrop™ Digital PCR System surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation, and multiplexing.Based on RainDance’s proven picodroplet platform, the RainDrop System delivers absolute quantitation of target molecules. Th…

Enclosed – safe – sound: Xiril’s Neon100 with Hood Xiril liquid handling robots are ideally suited to perform a large number of liquid handling tasks in automation solutions. E.g. processes such as nucleic acid separation or PCR setup can easily be automated. The proven pipetting robot Neon is available with up to 36 positions on the worktable, carrying one or two robotic arms facilitating your daily pipetting routine. The bas…

A 38µm soft non-permeable aluminum foil sealing film with strong medical grade adhesive, AlumaSeal II™ sealing films eliminate the need for heat-sealing devices or mats during thermal cycling. Compared to other aluminum foils, AlumaSeal II™ has less tendency to roll back on itself when removing the backing paper and conforms well to the plate during applications. •   Easily pierceable with single or multi-channel pipettors and…

Made of thin wall polypropylene and designed to fit on all Corning® Thermowell™ GOLD 1 x 8 well PCR strips and 96 well PCR microplates DNase- and RNase-free Autoclavable at 121°C

Thermo Scientific* Fermentas PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs and all the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR. ■ Convenient—Taq DNA Polymerase in a ready-to-use mix ■ Fast—sa…

The MagNA Pure LC Cooling Block, LC Centrifuge Adapters, holds 32 LightCycler® Centrifuge Adapters containing LightCycler® Capillaries (20 µl). The Cooling Block is designed to cool the capillaries during automated PCR setup by the MagNA Pure LC Instrument. In addition, the Cooling Block holds fifteen 1.5 ml reaction tubes (e.g., for master mixes). The stage of the MagNA Pure LC Instrument includes a cooling area, which is des…

The MagNA Pure LC Cooling Block, LC Sample Carousel holds one LightCycler® Sample Carousel (all formats) containing LightCycler® Capillaries. The Cooling Block is designed to cool the capillaries during automated PCR setup by the MagNA Pure LC Instrument. In addition, the Cooling Block holds sixteen 1.5 ml reaction tubes (e.g. for master mixes). The stage of the MagNA Pure LC Instrument includes a cooling area, which is design…