PCR and Thermal Cycling Products & Reviews

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Polymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.

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Mastercycler X40 - PCR Thermal Cycler®

Eppendorf

The Mastercycler X40 combines features that raise your standard in endpoint PCR to a new level. In addition to classic features such as a twelve-slit gradient and outstanding block temperature homogeneity, there is a simple, intuitive user interface, a touchscreen and a SafeLid lid that protects your samples from evaporation.

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Mastercycler® Services

Eppendorf

Amplify your performance To ensure your instrument is operating consistently at peak performance, we have developed service programs and options to meet your needs. Enjoy peace of mind with Eppendorf Performance Plans and Services for your Mastercycler pro, Mastercycler ep and Mastercycler family thermal cyclers. Performance Plans Features 1. Eppendorf service quality and integrity 2. Certified reports 3. Customized service op…

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icon96™

n6 Tec., Inc

Stop gambling on cycle numbers. The icon96 uses iconPCR™ technology with AutoNorm™ software to provide real-time fluorescence feedback in each of 96 independent wells, preventing under-amplification dropouts and over-amplification artifacts—delivering balanced, sequencing-ready libraries without manual quantification or normalization.

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LightCycler® Pro System

Roche

A qPCR system designed to deliver accuracy and flexibility to research and clinical diagnostic laboratories. Leveraging the robustness and proven technology of the LightCycler® 4801, the LightCycler® PRO integrates innovative technology and Roche manufacturing to deliver accurate real-time PCR results, flexible solutions to your workflow, and an improved user experience to your lab.

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CpG WIZ® p16 Amplification Kit

Merck

The CpG WIZ® p16 Amplification Kit is used for determining the methylation status of the p16 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified such that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and unmethylated)…

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CpG WIZ® p15 Amplification Kit

Merck

The CpG WIZ® p15 Amplification Kit is used for determining the methylation status of the p15 promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the p15 promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify template under the same conditions. Control genomic DNA samples (methylated and unm…

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CpG WIZ® E-Cadherin Amplification Kit

Merck

The CpG WIZ® E-cahedrin Amplification Kit is used for determining the methylation of status of the E-cadherin promoter by methylation-specific PCR (MSP). The kit contains primers targeted to regions of the promoter where the sequences are most divergent after bisulfite treatment. PCR parameters have been identified so that all primer sets in the kit amplify under the same conditions. Control genomic DNA samples (methylated and…

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CpG WIZ® Prader-Willi/Angelman Amplification Kit

Merck

The CpG WIZ® Prader-Willi/Angelman Amplification Kit is used for determining the methylation status of this region by methylation -specific PCR (MSP). The kit contains primers targeted to the CpG island on the SNRPN gene. PCR parameters have been identified such that both primer sets in the kit amplify template under the same conditions. Normal control genomic DNA, which will generate a product with both primer sets, is also i…

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CpGenome Universal Unmethylated DNA

Merck

CpGenome™ Universal Unmethylated DNA Set provides 2 separate genomic DNA controls for gene methylation studies. This product is intended for use with the CpGenome™ DNA Modification Kit (S7820) and can be subsequently used as an unmethylated DNA control for gene methylation studies.

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CpG WIZ® BRCA1 Amplification Kit

Merck

The CpGenome™ DNA Modification Kit (Cat. No. S7820) contains the reagents necessary to perform the initial bisulfite reactions, while the CpG WIZ® BRCA1 Amplification Kit contains the reagents required for the PCR amplification reactions.

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xxpress™ Thermal Cycler

BJS TECHNOLOGIES

With a uniform thermal ramp rate of over 10°C per second xxpress™ is the fastest real-time thermocycler in the World delivering 40 cycle q-PCR in under 10 minutes. And whilst not all PCR chemistries are yet as fast as this system there are a number that repeatably and reliably work at this speed. Ultra low thermal mass and high conductively enable the patented resistive heating system deliver this speed cycle more efficiently…

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Arrayed BAC library

Bio S&T

An arrayed or non-arrayed BAC library is the preferred choice for downstream applications such as hybridization screening; full-genome sequencing; BAC end sequencing or physical mapping projects. Amount of clones received is based on a guaranteed coverage and 120Kb average insert size. HMW DNA isolation and QC by HMW DNA enzymatic digestion are included. Deliverables for ARRAYED BAC library: Clones will be arrayed and amplifie…

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Screened BAC library

Bio S&T

A pooled BAC library is an invaluable tool for PCR-screening. It allows for the easy retrieval of BAC clones of interest. This type of library is especially useful when genome size is large and creating an arrayed library is cost-prohibitive. We typically construct a minimum 5X BAC library and BAC clones are then pooled and distributed in 96-well plates. Each well will contain about 400-500 original BAC clones. HMW DNA isolati…

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Normalized cDNA libraries

Bio S&T

Our cDNA libraries feature full-length-enriched and high quality size-fractionated cDNA. cDNA library construction is usually done in slightly-modified versions of pBluescript or pcDNA3.1. Insert sizes will range from about 0.5Kb to 4Kb. Concerning QC steps, we evaluate the average insert size, empty clone rate and clone concentration/tube before shipping out. We always provide a gel picture of randomly selected clones showing…

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ASH cDNA Subtracted Library

Bio S&T

ASH is APA-based, full-length-enriched, single-stranded cDNA subtraction hybridization library. - ASH cDNA subtraction is full-length-enriched and independent of PCR suppression. - ASH cloning is directional and does not involve normalization. - ASH method provides non-exponential amplification before subtraction because double-stranded cDNA is not required. - ASH method has a better subtraction effect because 1) a high ratio…

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RealTime Ready Assays

RealTime ready Custom RT-qPCR Assays and Panels Get a head start on your gene expression analysis with function tested assays for all annotated human, mouse, and rat genes Achieve high specificity using intron-spanning assays based on short hydrolysis LNA-substituted Universal ProbeLibrary (UPL) Probes.  Rely on stringently RT-qPCR-tested assays that are compliant with MIQE (Minimum Information for Publication of Quantitati…

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RainDrop™ Digital PCR System

RainDance Technologies, Inc.

A Billion Reactions. Digital Answers. Experience the Next Generation of PCR. Capable of generating more than a billion reactions in a single day, the RainDrop™ Digital PCR System surpasses all existing technologies and establishes a new performance standard in sensitivity, quantitation, and multiplexing.Based on RainDance’s proven picodroplet platform, the RainDrop System delivers absolute quantitation of target molecules. Th…

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Automated Pipetting - Neon 100 with Hood

Xiril AG

Enclosed – safe – sound: Xiril’s Neon100 with Hood Xiril liquid handling robots are ideally suited to perform a large number of liquid handling tasks in automation solutions. E.g. processes such as nucleic acid separation or PCR setup can easily be automated. The proven pipetting robot Neon is available with up to 36 positions on the worktable, carrying one or two robotic arms facilitating your daily pipetting routine. The bas…

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AlumaSeal II™ Sealing Film for PCR and Cold Storage

J.G. Finneran Associates, Inc

A 38µm soft non-permeable aluminum foil sealing film with strong medical grade adhesive, AlumaSeal II™ sealing films eliminate the need for heat-sealing devices or mats during thermal cycling. Compared to other aluminum foils, AlumaSeal II™ has less tendency to roll back on itself when removing the backing paper and conforms well to the plate during applications. •   Easily pierceable with single or multi-channel pipettors and…

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