Gene Expression and Molecular Cloning Products & Reviews

Pooled CRISPR screening with 10x genomics compatibility for discovery at single cell resolution

The Gator ® High Sensitivity (HS) AAV9 Kit enable high sensitivity quantification of AAV9 in crude and purified samples. The HS AAV9 Kit includes: HS AAV9 probes (96 probes/tray) HS AAV9 Detection Solution AAV Amplification Solution AAV Substrate Solution AAV Substrate Diluent Q Buffer ** Contains temperature sensitive components. Must be shipped overnight. ** *Not compatible with Gator ® AAV9 probe (Part No. 160021)

This product selection tool identifies your best solution for high transfection performance

Designed for exceptional DNA delivery efficiency into hard-to-transfect, primary, and cancer cell lines.

The most trusted delivery brand now offering cutting-edge lipid nanoparticle (LNP) solutions with proven safety, delivery efficiency and specificity for your nucleic acid drug delivery needs.

Hybridizes to crRNA to activate the Cas9 enzyme.

Hybridizes to crRNA to activate the Cas9 enzyme.

The first viral feed designed specifically for HEK293 cells

The Gator ® AAV Ratio Kit enables empty vs full determination of AAV serotypes 1-10 and chimeric serotypes in crude and purified samples. The AAV Ratio Kit includes: AAVX probes (96 probes/tray) SSB probes (96 probes/tray) Lysis Buffer DNA Detection Solution AAV Ratio Substrate Q Buffer SS DNA Standard Lysis tubes ** Contains temperature sensitive components. Must be shipped overnight. **

Complete ligation reactions in just 15 minutes, efficient cloning at low concentrations of DNA, increased productivity and efficiency.

A simple 7-minute sample preparation procedure is the first part of a complete workflow provided by the TaqMan® Gene Expression Cells-to-CT™ Kit. Remove the guesswork of assembling separate RNA purification, RT, and real-time PCR kits, and achieve unsurpassed performance and sensitivity in gene expression analysis, including detection of siRNA-induced mRNA knockdown. - Complete solution—Optimized workflow includes cell lysis r…

X-tremeGENE siRNA Transfection Reagent efficiently delivers short interfering RNA (siRNA) into many commonly used cell types including HeLa, NIH 3T3, HEK-293, CHO-K1, and COS-7, and several hard-to-transfect cell lines such as HT29, a human adenocarcinoma cell line. When used for cotransfection-based gene knockdown, X-tremeGENE siRNA Transfection Reagent enables significant protein expression and effective knockdown levels due…

Upgrade from the Rapid DNA Ligation Kit and choose the new Rapid DNA Dephos & Ligation Kit for fast and efficient dephosphorylation and ligation of sticky- or blunt-end DNA fragments. The convenient kit enables ligation of DNA in 5 minutes and dephosphorylation in 10 minutes. This kit contains the new recombinant rAPid Alkaline Phosphatase, the tool of choice for dephosphorylation of 5' phosphates from nucleic acids. This kit…

The Transcriptor First Strand cDNA Synthesis Kit is designed to reverse transcribe RNA (mRNA, total RNA, viral RNA, and in vitro-transcribed RNA) from a variety of sources for the following applications: Studying gene expression levels, via two-step RT-PCR, using qualitative RT-PCR or quantitative RT-PCR on the LightCycler® Carousel-Based System, the LightCycler® 480 System, or other real-time instruments. Generating cDN…

The Transcriptor High Fidelity cDNA Synthesis Kit is designed to reverse transcribe RNA with an increased fidelity compared to other reverse transcriptases. The kit features Transcriptor High Fidelity Reverse Transcriptase, a blend of a recombinant reverse transcriptase and a proofreading mediating enzyme optimized for two-step RT-PCR, making this the product of choice for the following applications: Cloning genes of inter…

Dual-Light® luminescent reporter gene assay is designed for the rapid and sensitive detection of firefly luciferase and b-galactosidase in a single extract aliquot for gene expression assays. Detection of firefly luciferase and b-galactosidase in the same sample simplifies normalization of transfection efficiency Wide dynamic range of b-galactosidase and luciferase assays enables accurate measurement of both enzymes from…

The Galacto-Light™ beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of b-galactosidase in reporter gene assays.

The Galacto-Light Plus™ beta-Galactosidase Reporter Gene Assay System is designed for the rapid, ultrasensitive detection of b-galactosidase in reporter gene assays.

The Galacto-Star™ One-Step beta-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of B-galactosidase in cell lysates.

The Gal-Screen® assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.

The Luc-Screen® assay system with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme.

The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression.

Electroporation with an effective buffer and optimized conditions can provide a highly efficient method for transfecting primary and hard-to-transfect cells with siRNAs. The siPORT™ siRNA Electroporation Buffer/Kit can be used with multiple types of electroporators to ensure highly efficient delivery of siRNA into cells while maintaining high cell viability.

siPORT XP-1 is an easy-to-use reagent that efficiently delivers both plasmid DNA and PCR products into a variety of mammalian cell types. Comprised of a proprietary formulation of polyamines, the reagent exhibits low toxicity and can be used both in the presence or absence of serum. siPORT XP-1 is particularly straightforward to use. Reagent/DNA complexes can be added directly to growing cells in serum-containing culture med…