High performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The OPTI-SOLV® Mini Filter provides low-impact filtration in a package no larger than a finger tight fitting. Use them to extend the life of your analytical column, or before your mass spectrometer as a last line of defense against debris. Perfect zero-dead-volume connections are obtained every time with our patented automatic tube stop depth adjustment. Unique depth-filtering elements allow for greater capacity with no band s…
The OPTI-SOLV® Nano Filter follows our Micro Filter but cuts the internal volume down even further. With less than 50 nL of internal volume, the OPTI-SOLV® Nano Filter is ideal for mass spectrometry to protect your electrospray and nanospray tips from clogging with debris. Like the Micro and Mini Filter, the Nano Filter is designed to be used in today's low volume, high sensitivity chromatographic applications as a last line o…
For your volume critical filtering needs, we now offer the OPTI-SOLV® Micro Filter. Based on the same design that has made the Mini Filter a huge success, the Micro Filter cuts the internal volume to less than 200 nL but retains the ease of use and functionality of the Mini Filter. The Micro Filter is designed to be used in today's low volume, high sensitivity chromatographic applications as a last line of defense against debr…
The user-friendly Hand-Tight OPTI-SOLV® In-Line Filter System is well suited for a number of applications, such as high temperature, high-pressure environments where quick changes are imperative. The unique swivel design allows frits to be changed without removing the connecting tubing. Just plumb it once and leave it. The frictional forces generated at the piston/seal interface inevitably lead to particulate shedding. Placeme…
The user-friendly Wrench-Tight OPTI-SOLV® In-Line Filter System is well suited for a number of applications, such as high temperature, high-pressure environments where quick changes are imperative. The unique swivel design allows frits to be changed without removing the connecting tubing. Just plumb it once and leave it. The frictional forces generated at the piston/seal interface inevitably lead to particulate shedding. Place…
OPTI-GUARD® 1mm Biocompatible Guard Columns set the standard for extremely low impact, easy to use pre-column protection. Designed for use with analytical (4.6, 3.0 mm i.d.) and narrow-bore (2.1, 1.0 mm i.d.) columns, the patented floating stem design automatically adjusts to any manufacturer's tube stop depth for a zero-dead-volume connection every time. The best part? It's no larger than a standard fitting. Connecting the OP…
The OPTI-GUARD® 3 mm Columns maintains the tool-free connectivity of the OPTI-GUARD 1 mm, but incorporates a cartridge based format to allow for a larger bed with more capacity. The two-part holder is designed for use with any analytical column to provide vital protection from dirty samples and strongly retained contaminants. An auto-adjusting stem conforms to any tube stop depth for a perfect zero-dead-volume connections.Key…
OPTI-SEAL® Piston/Plunger Seals are the longest lasting seals on the market. Machined from either of two proprietary polymer blends, UHMW-PE or ITB, Optimize Seals provide the ultimate in high pressure sealing capacity. For mostly aqueous buffered mobile phases, OPTI-SEAL® UHMW-polyethylene is the wisest choice. Specially designed to be more resistant to abrasion and wear than standard polyethylene seals, they can last up to s…
Optimize realizes that your time is valuable and should not be wasted worrying about fittings. For this reason, all Opti-Lock Fittings are precision machined in the USA for optimal quality and thread consistency. The machining process is the best way to ensure tight manufacturing tolerances and minimal product variance. What does all this mean? It means that every fitting you purchase from Optimize will perform just as well as…
Optimize Back Pressure Regulators utilize an active mechanism to maintain steady back pressure across a range of mobile phase viscosities and flow rates. Placing one downstream from your detector will prevent solvent decompression and bubble formation in the flow cell, reducing baseline noise and drift. Each pre-set Optimize stainless steel Back Pressure Regulator offers a 100µL internal volume and includes fittings for immedi…
Optimize Replacement Pistons/Plungers are, by design, a consumable part of the HPLC pump and should be replaced on a regular preventative maintenance schedule. To maintain peak performance and avoid unplanned downtime, sapphire pistons require inspection every six months with yearly or as-needed replacement. Optimize sapphire pistons offer optimal concentricity and resistance to wear. Each sapphire rod is specifically cut in r…
The OPTI-LYNX™ Quick Connect Filters allow you to connect any 10-32 valve/port for trapping or analytical column for pre-column filtration. With OPTI-LYNX™, accessing and changing the insert takes only a quarter turn. Replace traps, filters or change configurations in seconds, without tools. OPTI-LYNX™ traps and filters (ordered separately) come in several bed volume/ diameter/ porosity combinations, allowing you to find your…
The OPTI-LYNX™ Quick Connect Trap Columns allow you to quick-connect to any 10-32 valve/port for trapping or analytical column for pre-column filtration. With OPTI-LYNX™, accessing and changing the insert takes only a quarter turn. Replace traps, filters or change configurations in seconds, without tools. OPTI-LYNX™ traps and filters (ordered separately) come in several bed volume/ diameter/ porosity combinations, allowing you…
The OPTI-LYNX™ QuickConnect Guard Column is a high-performance, cartridge-based guard system, suitable for use with any 10-32 analytical column due to its auto-adjusting ZDV port adapter. OPTI-LYNX™ combines effective column protection with the convenience of quick-connect operation. Some cartridge-based guard column products offer convenience with a price: higher internal volumes. Optimize Technologies, refuse to compromise p…
The LaChromUltra® U-HPLC System, in combination with the TQ Detector, provides unmatched quantitative analysis and characterization capabilities. Features • Seamless integration of the U-HPLC system and the TQ Detector with Empower™ 2 control • Performs SIR, MRM, Product, Precursor, and Neutral Loss Scans on the U-HPLC timescale • Equipped with dual ESI/APCI source as standard, with optional APPI available • 20 ms polarity swi…
The Hitachi HPLC Primaide is designed for long-term, stable operation, and features both high reliability and great durability. It offers tremendous capability for everyday analysis.Based on over 50 years of experience in the development and production of HPLC systems, Hitachi proudly introduces the Primaide. Make the most of your analytical equipment with Hitachi as the “Prime Aide” in your lab! Modules • Primaide 1110 Pump w…
LyoSpeed™ delivers exceptional results with even the most difficult samples. When working with certain solvents, e.g. HPLC solvents, a fully dried result can be hard to achieve due to interactions with the dissolved sample - resulting in the formation of a gum or oil.In response to customer demand Genevac have developed a Fast Lyophilisation, or LyoSpeed™ process to resolve these problems for customers working with HPLC solve…
The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support.Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, the Ultra Amino Columns (USP L8) are designed for selective and reliable HPLC applications. Particle size: 3 μm or 5 μm, spherical Pore size: 100 Å Carbon load: 2% End-cap: no pH range: 2.5 to 8 Temperature limit: 80 °C USP phase code…
The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support. Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, the Ultra Cyano Columns (USP L10) are designed for selective and reliable HPLC applications.Particle size: 3 μm or 5 μm, spherical Pore size: 100 Å Carbon load: 8% End-cap: yes pH range: 2.5 to 8 Temperature limit: 80 °C USP phase co…
The Ultra line represents Restek’s broadest selection of stationary phases on a single silica support. Made of high-purity, type-B silica that minimizes activity and creates high-density bonding, Ultra Silica Columns (USP L3) are designed for selective and reliable HPLC applications. Particle size: 3 μm or 5 μm, spherical Pore size: 100 Å End-cap: no pH range: 2.5 to 8 Temperature limit: 80 °C USP phase code: L3 Phase catego…
Viva columns are based on a wide pore material we designed for optimal large-molecule separations.In developing Viva silica, we found that although many commercial wide-pore silicas meet the standard 300 Å mean pore size, most have very broad distributions about this mean, with a significant portion of their pore volume falling below 150 Å.This means a large portion of the surface area is unavailable to larger molecules. Viva…
Use our bulk packing materials to pack your own columns. Our extensive QC program ensures the high quality and reproducibility of these silicas. You can be confident that you are getting consistent, high-quality product when you source your silica from Restek. Use these materials for easy scale-up to preparative chromatography or for packing your own columns. Ultra Bulk Packing Materials Features: Prepare your own columns…
Eshmuno® A is a rigid, high capacity Protein A affinity chromatography resin for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies. Eshmuno® A resin can be cleaned and sanitized under acid and alkaline conditions while maintaining high dynamic binding capacity at high flow rates.The Eshmuno® A resin contains a EMD Millipore proprietary ligand. This ligand is recombinantely expresse…
The PDS-100 is a Programmable Dispensing System which uses precision stepper motors to control FMI “Q” and “RH” pump models including STQP, STQ, STH, & STRH in all piston sizes. The FMI pumps are integrally mounted to the programmable controller. The controller contains the stepper motors and driver electronics, and is a rugged anodized aluminum design. Several single and dual pump configurations are available including a dua…
PiP Pumps micro π-petter - Pipetting, Syringing and Diluting Ideal for repetitive and volumetric dispensing of acids, solvents and aqueous solutions. Model "PiP" Precision Dilutor Features: FMI's unique low dead-volume pump heads, and synchronous motor drives. Can act as a single shot dispenser using the hand/foot switch or as a single metering pump in the continuous mode. Using a combination of forwar…
