Molecular cloning is a set of techniques that utilizes vectors to transfer recombinant DNA into host cells and is an essential tool for investigating the expression of genes and proteins in bacterial or mammalian cells. A variety of vectors optimized for gene cloning and expression in a range of host organisms are available, alongside competent cells for genetic replication. Here, you can explore a range of molecular tools, high-quality genomic and cDNA libraries, premade clones, transformation and transfection reagents and mutagenesis or gene expression detection assays and expression arrays. Find the best gene expression and molecular cloning products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
The Galacto-Star™ One-Step beta-Galactosidase Reporter Gene Assay System chemiluminescent reporter gene assay system enables rapid, sensitive detection of B-galactosidase in cell lysates.
The Gal-Screen® assay system combines direct cell lysis with rapid, ultra-sensitive chemiluminescent detection of β-galactosidase reporter enzyme.
The Luc-Screen® assay system with extended-glow light emission is designed for sensitive detection of firefly luciferase reporter enzyme.
The pMIR-REPORT™ miRNA Expression Reporter Vector System provides accurate, quantitative, in-cell measurement of miRNA expression.
Electroporation with an effective buffer and optimized conditions can provide a highly efficient method for transfecting primary and hard-to-transfect cells with siRNAs. The siPORT™ siRNA Electroporation Buffer/Kit can be used with multiple types of electroporators to ensure highly efficient delivery of siRNA into cells while maintaining high cell viability.
siPORT XP-1 is an easy-to-use reagent that efficiently delivers both plasmid DNA and PCR products into a variety of mammalian cell types. Comprised of a proprietary formulation of polyamines, the reagent exhibits low toxicity and can be used both in the presence or absence of serum. siPORT XP-1 is particularly straightforward to use. Reagent/DNA complexes can be added directly to growing cells in serum-containing culture med…
The DyNAmo™ cDNA Synthesis Kit is intended for cDNA synthesis for two-step quantitative reverse transcription-PCR (qRT-PCR) applications. This kit can be used in conjunction with Finnzymes' DyNAmo qPCR Kits or with any other qPCR kit suitable for the application.The DyNAmo cDNA Synthesis Kit includes all necessary reagents for cDNA synthesis to be used in qPCR. Either total RNA, messenger RNA, viral RNA or in vitro transcribed…
The Phusion™ Site-Directed Mutagenesis Kit is a versatile and efficient tool for introducing point mutations, insertions or deletions in any type of plasmid DNA. With this kit, the entire plasmid is amplified using phosphorylated primers that introduce the desired changes. The amplified, linear PCR product, containing the desired mutation, is circularized in a 5-minute ligation reaction with Quick T4 DNA Ligase. The resulting…
The Best Transfection Reagent. Applicable to cells that are difficult to transfect. DNAfectin™ is specially formulated with multiple cationic polymers. Application: Transfection of DNA into cultured eukaryotic cells.
The Thermo Scientific Verso system achieves robust and sensitive reverse transcription through the combination of a high affinity RT enzyme, a unique RNA priming method, and an optimized buffering system. The Verso RT enzyme has high RNA template affinity and reduced RNase H activity, to transcribe even sections with high secondary structure. The included anchored oligo dT priming method further enhances sensitivity by increas…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Apoptosis and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate,…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Cancer and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Re…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Cardiovascular Disease and related genes reformatted onto an OpenArray plate. Kit includes: Ope…
OpenArray® Pathways are ready-to-use gene panels containing pre-validated real-time qPCR assays. The plates have over 600 assays each and are compatible with multiple sample types. Panels are currently avaliable with human or mouse genes. New panels are continually under development. Check back for updates. Real-Time qPCR assays for Inflammation and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray pla…
Real-Time qPCR assays for Signal Transduction and related genes reformatted onto an OpenArray plate. Kit includes: OpenArray plate, Real-Time qPCR Case, Immersion Fluid, Frame Adhesive and Plate-File CD.
Applied Biosystems currently offers five TaqMan Copy Number Assays to detect copy number variation. Copy number is an important polymorphism in the human genome associated with genetic diseases such as cancer, immune diseases, and neurological disorders. TaqMan Copy Number Assays were designed to detect CYP2D6, CYP2A6, CYP2E1, GSTT1, and GSTM1.
The TaqMan® Custom Array is a 384-well micro fluidic card that enables a researcher to perform 384 simultaneous real-time PCR reactions without the need to use liquid-handling robots or multichannel pipettors to load. This low- to medium- throughput array allows for 1-8 samples to be run in parallel against 12-384 TaqMan® Gene Expression Assay targets that are pre-loaded into each of the wells on the array. The TaqMan Custom…
The TaqMan Custom Plating service offers the convenience of pre-plated TaqMan® Gene Expression Assays, Custom Assays, and Custom Probe/Primer Sets in 96- or 384-well plates. This cost-effective custom plating service gives you the flexibility and reliability you need for your screening applications. Whether you are looking for biomarkers, testing for toxicity, searching for microbes, or performing other high-throughput scree…
Applied Biosystems TaqMan Drug Metabolism Genotyping Assays are available for basic research and clinical research. Genotype over 2,600 high value polymorphisms located in regulatory elements and coding regions for 220 drug metabolism and transporter genes with TaqMan® Assay technology.
TaqMan® Express Plates are a cost-effective and convenient 96-well format complimenting Applied Biosystem's line of individual TaqMan® Gene Expression Assays, and pre-spotted 384-well TaqMan® Arrays. Customize any of the six available plate formats with dried-down TaqMan® Assays by positioning each in specific well locations. This flexibility enables an efficient means to screen biological samples for the presence and amount…
TaqMan® Gene Sets are pre-defined lists of genes grouped by pathway or disease target class which can be ordered separately, as a custom plate, or configured directly onto TaqMan® Arrays to enable you to perform hundreds of real-time PCR reactions simultaneously on the Applied Biosystems 7900HT Fast Real-Time PCR System. Simple to implement, TaqMan Gene Sets are customizable, and when placed on a TaqMan Array, require mini…
TaqMan® Gene Signature Arrays enable analysis of hundreds of TaqMan® Gene Expression Assays on a 384-well micro fluidic card with minimal effort. TaqMan Gene Signature Arrays are readily available and delivered 2–4 days after ordering. They are packaged conveniently with two or four arrays per pack, making them cost effective, and just the right size for any experiment. Choose from pathway-specific pre-defined arrays such…
TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. The assays target only mature miRNAs, not their precursors, ensuring biologically relevant results. Get gold standard TaqMan Assay performance for miRNA quantitation and profiling…
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance using Applied Biosystems TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
