Gene Expression and Molecular Cloning Products & Reviews

The Human CRISPR inhibition (CRISPRi) library (Dolcetto) is a single compact library that inhibits over 18,000 human genes and is used for genome-wide inhibition screening using a KRAB-dCas9 effector.

The 10x Compatible Human CRISPRi Kinase Phosphatase Drug Targets Kit (Druggable Genome) contains one sub-pool of the top two ranked sgRNAs per gene for increased sensitivity and includes non-targeting controls built-in.

The Human CRISPR activation library (Calabrese) activates over 18,000 human genes and is used for genome-wide activation screening. The library is designed to be compact and efficient to maximize screening efficiency and performance, as published by Sanson, K.R., et al. Nat Commun 9, 5416 (2018).

The mouse CRISPR activation library (Caprano) activates over 22,000 mouse genes and is used for genome-wide activation screening.

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The control kit includes validated positive control targeting RAB1A and non-targeting control for assay set-up and a UCOE KRAB-dCas9 for consistent effector expression across a wide variety of cell lines. The kit is ideal for setting up the screen assay and optimization before using the CRISPRi library pools.

The 10X CRISPRi Feature Barcode Optimization kit is ideally suited for setting up the screen assay and if desired capture sequence optimization before using the Custom 10x Compatible CRISPRi library pools.

This pooled library targets the human kinome, a collection of approximately 700 genes commonly associated with development and disease, with an average of nearly 9 unique knockout CRISPR clones per kinase gene. The lentiviral format allows for stable integration, selection, and enrichment of transduced cells

Utilize the power of CRISPR Whole Genome Lentiviral Pools to knockout and screen every gene in the human genome. The Sigma Whole Human Genome Lentiviral CRISPR Pool is provided as 200 ul in 8 x 25 ul aliquots at a minimum titer of 5x108 viral particles/ml via p24

Accomplish highly effective transfection of CRISPR/Cas9 DNA, RNA, Cas9-gRNA RNP (ribonucleoprotein) complexes and siRNA to knock-out, modify or knock-down genes of interest in a wide variety of cell types.

Recombinant Cas9-D10A Nickase protein from Streptococcus pyogenes (~160 KD) is a ready-to-use reagent for genome engineering experiments.

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. This vector is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.

Human CRISPRi Kinase Phosphatase Drug Targets Kit contains 2 sub-pools of top 5 gRNAs per gene as well as a separate sub-pool of 5 supplemental gRNAs per gene for increased sensitivity and includes non-targeting controls built in.

The Lenti CRISPR Non-Target Control#1 (LV04 vector) is a lentiviral plasmid vector, which includes a gRNA sequence that does not target known human, mouse and rat genes. The DNA format of this control construct permits direct transfection in knockout experiments or use in the viral packaging process (for lentiviral-based knockdown experiments).

The Sigma lenti CRISPR Non-Targeting Control is a lentiviral system, which includes a gRNA sequence that does not target known human, mouse and rat genes. The non-targeting control lentivirus is useful as a negative control in experiments using Sigma CRISPR lentiviral clones.

Pooled CRISPR screening with 10x genomics compatibility for discovery at single cell resolution

The Gator ® High Sensitivity (HS) AAV9 Kit enable high sensitivity quantification of AAV9 in crude and purified samples. The HS AAV9 Kit includes: HS AAV9 probes (96 probes/tray) HS AAV9 Detection Solution AAV Amplification Solution AAV Substrate Solution AAV Substrate Diluent Q Buffer ** Contains temperature sensitive components. Must be shipped overnight. ** *Not compatible with Gator ® AAV9 probe (Part No. 160021)

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Designed for exceptional DNA delivery efficiency into hard-to-transfect, primary, and cancer cell lines.

The most trusted delivery brand now offering cutting-edge lipid nanoparticle (LNP) solutions with proven safety, delivery efficiency and specificity for your nucleic acid drug delivery needs.

Hybridizes to crRNA to activate the Cas9 enzyme.

Hybridizes to crRNA to activate the Cas9 enzyme.

The first viral feed designed specifically for HEK293 cells

The Gator ® AAV Ratio Kit enables empty vs full determination of AAV serotypes 1-10 and chimeric serotypes in crude and purified samples. The AAV Ratio Kit includes: AAVX probes (96 probes/tray) SSB probes (96 probes/tray) Lysis Buffer DNA Detection Solution AAV Ratio Substrate Q Buffer SS DNA Standard Lysis tubes ** Contains temperature sensitive components. Must be shipped overnight. **

Complete ligation reactions in just 15 minutes, efficient cloning at low concentrations of DNA, increased productivity and efficiency.