Products & Reviews

N-2 Supplement is a chemically-defined, serum-free supplement based on Bottenstein’s N-1 formulation. It is recommended for growth and expression of neuroblastomas as well as post-mitotic neurons in primary cultures from both the peripheral nervous system and the central nervous system. N-2 Supplement is provided as a 100X concentrate and is intended for use with Neurobasal Medium supplemented with growth factors such as bFGF…

Cyto-Chex BCT is a direct draw blood collection tube that maintains cellular morphology and surface antigen expression, including cluster of differentiation (CD) markers, prior to analysis by flow cytometry. Whole blood samples collected in Cyto-Chex BCT can be stored at room temperature for up to 14 days.   Cyto-Chex BCT has been FDA 510(k) cleared for consistent recovery of HIV-associated lymphocyte subsets (CD3, CD4,…

Western blotting is a widely used technique to detect levels of protein expression in cell or tissue extract. This technique measures protein levels in a biological sample through antibody binding to a specific protein of interest.   Features: Detects Small Amounts of Protein – delivers a sensitive and robust signal for standard protein detection Convenient, Cost-effective 500 ml Sizing – means you can p…

The CHEMICON® Alpha/Beta Integrin-Mediated Cell Adhesion Array Combo Kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Alpha/Beta Integrin-Mediated Cell Adhesion Arrays can be completed within 1-2 hours, providing a rapid, sensitive, and quantitative assay…

EMD Millipore's CXCR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CXCR1 interaction with IL-8. The membrane preparations exhibit a Kd of 0.2 nM for [125I]-IL-8. With 5 μg/well CXCR1 Membrane Prep and 0.1 nM [125I]-IL-8, a greater than 5-fold signal-to-…

EMD Millipore's D2 dopamine membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of D2 dopamine receptor interactions with its ligand. The membrane preparations exhibit a Kd of 0.51nM for [125I]-Iodospiperone. With 5.0 ug/well D2 Dopamine Membrane Prep and 0.1nM […

Chemicon's β2 adrenoceptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of β2 adrenoceptor interactions with (-)Iodocyanopindolol (ICYP). The membrane preparations exhibit a Kd of 0.51 nM for [125I]-(-)ICYP. With 5 µg/well β2 Adrenoceptor Membrane Prep and…

EMD Millipore's β1 adrenoceptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of β1 adrenoceptor interactions. The membrane preparations exhibit a Kd of 2.6 nM for [125I]-(-) Iodocyanopindolol (ICYP). With 5 µg/well β1 Adrenoceptor Membrane Prep and 0.25 nM…

Chemicon's glucagon receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of glucagon receptor interactions and its ligands. The membrane preparations exhibit a Kd of 3.09 nM for [125I]-glucagon. With 1 nM [125I]-glucagon, 5 or 10 μg/well glucagon receptor…

Chemicon's GABAB1b membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of GABAB1b receptor interactions with its ligand. The membrane preparations exhibit a Kd of 1.15nM for [3H]-CGP 54626A. With 5.0 ug/well GABAB1b Membrane Prep and 7.5nM [3H]-CGP 54626A, a grea…

Chemicon's EP4 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of EP4 interactions with prostaglandin E2. The membrane preparations exhibit a Kd of 1.27-1.85 nM for [3H]-Prostaglandin E2. With 5 µg/well EP4 Membrane Prep and 1.5 nM [3H]-Prostaglandin E2, a gr…

EMD Millipore's Ghrelin Receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of ghrelin interactions and its ligands. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-ghrelin. With 0.1 nM [125I]-ghrelin, 5 μg/well Ghrelin Receptor Membrane Pre…

New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction.NEBNext® dsDNA Fragmentase Reaction Buffer Components: 20mM Tris…

NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Pr…

The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressi…

The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents includ…

ChIP, ChIP-seq, and methyl antibodies for stem cell studies. In recent years, studies suggest that the mammalian genome undergoes various epigenetic modifications during development and embryonic stem cell (ESC) differentiation. Understanding the mechanisms that control these epigenetic signals is essential in deciphering stem cell biology and the patterns of gene expression during cellular differentiation. ESC gene express…

New recombinant Alkaline PhosphataserAPid Alkaline Phosphatase is used to dephosphorylate 5' ends of DNA and RNA. Alkaline phosphatase treatment prevents self-ligation of fragments by removing the 5'-phosphoryl termini required by ligases; this feature is of major importance in cloning strategies to decrease vector background. rAPid Alkaline Phosphatase can also be employed to dephosphorylate proteins. Use rAPid Alkaline Phosp…

Millipore’s The FlowCellect™ Human Embryonic Stem Cell Characterization kit is designed as an easier and quicker way to track surface marker expression of hOCT4, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both hOCT4 and SSEA4 and not SSEA1. This quick test will allow researchers to determine th…

Millipore’s The FlowCellect™ Human Embryonic Stem Cell New Surface Marker Characterization Kit is designed to facilitate an easier and quicker way to track surface marker expression of HESCA1, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both HESCA1 and SSEA4 and not SSEA1. This quick test will a…

Chemically defined cell culture mediums. With the introduction of Cellvento™ CHO media and Cellvento™ Feed products, Merck Millipore offers you chemically defined, non-animal origin media formulations for batch and fed-batch mode operations. The media and feeds are specially designed for consistent, high performance results in Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or recombinant prot…

ProteinPilot software is a powerful, robust and intuitive software tool for protein identification and quantification for discovery research and protein characterization.  A paradigm shift in protein identification and relative protein expression analysis for protein research, ProteinPilot software streamlines protein identification and quantitation, enabling you to identify hundreds of peptide modifications and non-tryptic cl…

Learn more about using Lambda gt11 Vector Kits to construct cDNA libraries. The Lambda gt11 expression vector accepts inserts up to 7.2 kilobases at a unique EcoR I site near the C-terminal end of the coding region of the lacZ gene. DNA sequences cloned into the EcoR I site of the vector may be expressed as fusion proteins under the control of the lac promoter. Libraries constructed in this vector can be screened with both an…

Chemicon's CB1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists at CB1. The membrane preparations exhibit a Kd of 7 nM for [3H]-SR141716A. In the presence of 2 nM [3H]-SR141716A with CP55940 as unlabeled competitor, 5 μg/well and 10 μg/well CB1 M…

Chemicon's A1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of A1 interactions with its ligands. The membrane preparations exhibit a Kd of 1.2 nM for [3H]-Cyclopentyl-1, 3-dipropylxanthine, 8-[dipropyl-2, 3-3H (N)]. With 5mg/well A1 Membrane Prep and 20nM […