Products & Reviews

Chemicon's β2 adrenoceptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of β2 adrenoceptor interactions with (-)Iodocyanopindolol (ICYP). The membrane preparations exhibit a Kd of 0.51 nM for [125I]-(-)ICYP. With 5 µg/well β2 Adrenoceptor Membrane Prep and…

EMD Millipore's β1 adrenoceptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of β1 adrenoceptor interactions. The membrane preparations exhibit a Kd of 2.6 nM for [125I]-(-) Iodocyanopindolol (ICYP). With 5 µg/well β1 Adrenoceptor Membrane Prep and 0.25 nM…

Chemicon's glucagon receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of glucagon receptor interactions and its ligands. The membrane preparations exhibit a Kd of 3.09 nM for [125I]-glucagon. With 1 nM [125I]-glucagon, 5 or 10 μg/well glucagon receptor…

Chemicon's GABAB1b membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of GABAB1b receptor interactions with its ligand. The membrane preparations exhibit a Kd of 1.15nM for [3H]-CGP 54626A. With 5.0 ug/well GABAB1b Membrane Prep and 7.5nM [3H]-CGP 54626A, a grea…

Chemicon's EP4 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of EP4 interactions with prostaglandin E2. The membrane preparations exhibit a Kd of 1.27-1.85 nM for [3H]-Prostaglandin E2. With 5 µg/well EP4 Membrane Prep and 1.5 nM [3H]-Prostaglandin E2, a gr…

EMD Millipore's Ghrelin Receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of ghrelin interactions and its ligands. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-ghrelin. With 0.1 nM [125I]-ghrelin, 5 μg/well Ghrelin Receptor Membrane Pre…

New England Biolabs supplies a 10X reaction buffer with all of its enzymes. At a 1X concentration this reaction buffer assures optimal activity of the enzyme. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction.NEBNext® dsDNA Fragmentase Reaction Buffer Components: 20mM Tris…

NEBNext® reagents are a series of highly pure reagents that facilitate library preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. Available in sets, master mixes and modules, these robust reagents undergo stringent quality controls and functional validation, ensuring maximum yield, convenience and value. Advantages of NEBNext Reagents for RNA Library Pr…

The NEBNext® DNA Library Prep Master Mix Set for Illumina® contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressi…

The NEBNext mRNA Library Prep Master Mix Set for 454 contains enzymes and buffers that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expression library construction. The reagents includ…

ChIP, ChIP-seq, and methyl antibodies for stem cell studies. In recent years, studies suggest that the mammalian genome undergoes various epigenetic modifications during development and embryonic stem cell (ESC) differentiation. Understanding the mechanisms that control these epigenetic signals is essential in deciphering stem cell biology and the patterns of gene expression during cellular differentiation. ESC gene express…

New recombinant Alkaline PhosphataserAPid Alkaline Phosphatase is used to dephosphorylate 5' ends of DNA and RNA. Alkaline phosphatase treatment prevents self-ligation of fragments by removing the 5'-phosphoryl termini required by ligases; this feature is of major importance in cloning strategies to decrease vector background. rAPid Alkaline Phosphatase can also be employed to dephosphorylate proteins. Use rAPid Alkaline Phosp…

Millipore’s The FlowCellect™ Human Embryonic Stem Cell Characterization kit is designed as an easier and quicker way to track surface marker expression of hOCT4, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both hOCT4 and SSEA4 and not SSEA1. This quick test will allow researchers to determine th…

Millipore’s The FlowCellect™ Human Embryonic Stem Cell New Surface Marker Characterization Kit is designed to facilitate an easier and quicker way to track surface marker expression of HESCA1, SSEA4 and SSEA1. The kit will also help to determine the percentage of undifferentiated human embryonic stem cells in culture by determining the percentage of cells that express both HESCA1 and SSEA4 and not SSEA1. This quick test will a…

Chemically defined cell culture mediums. With the introduction of Cellvento™ CHO media and Cellvento™ Feed products, Merck Millipore offers you chemically defined, non-animal origin media formulations for batch and fed-batch mode operations. The media and feeds are specially designed for consistent, high performance results in Chinese Hamster Ovary (CHO) cells in suspension for the expression of antibodies or recombinant prot…

ProteinPilot software is a powerful, robust and intuitive software tool for protein identification and quantification for discovery research and protein characterization.  A paradigm shift in protein identification and relative protein expression analysis for protein research, ProteinPilot software streamlines protein identification and quantitation, enabling you to identify hundreds of peptide modifications and non-tryptic cl…

Learn more about using Lambda gt11 Vector Kits to construct cDNA libraries. The Lambda gt11 expression vector accepts inserts up to 7.2 kilobases at a unique EcoR I site near the C-terminal end of the coding region of the lacZ gene. DNA sequences cloned into the EcoR I site of the vector may be expressed as fusion proteins under the control of the lac promoter. Libraries constructed in this vector can be screened with both an…

Chemicon's CB1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists at CB1. The membrane preparations exhibit a Kd of 7 nM for [3H]-SR141716A. In the presence of 2 nM [3H]-SR141716A with CP55940 as unlabeled competitor, 5 μg/well and 10 μg/well CB1 M…

Chemicon's A1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of A1 interactions with its ligands. The membrane preparations exhibit a Kd of 1.2 nM for [3H]-Cyclopentyl-1, 3-dipropylxanthine, 8-[dipropyl-2, 3-3H (N)]. With 5mg/well A1 Membrane Prep and 20nM […

Chemicon's CXCR6 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CXCR6 interactions with its ligands. The membrane preparations exhibit a Kd of 0.6 nM for [125I]-CXCL16. With 2.5 or 10 μg/well CXCR6 Membrane Prep and 0.5 nM [125I]-CXCL16, greater than 4-fo…

Chemicon's EP3 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of EP3 interactions and its ligands. The membrane preparations exhibit a Kd of 1.56 nM for [3H]-PGE2. With 1.5 nM [3H]-PGE2, 5 µg/well and 10 µg/well EP3 Membrane Prep typically yield greater than…

Chemicon's mGlu2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of mGlu2 interactions with its ligands. The cell line exhibits a calcium response with EC50s of 0.51uM, 5.6uM, and 8.3uM for DCG IV, (2R4R) APDC, and glutamate. The membrane preparations exhibit EC50s of of 0.…

SilicoCyte™ is an advanced, modular, flexible and highly integrated microarray analysis platform designed for automated image analysis, comprehensive data annotation, elaborate data analysis and advanced data visualization.It has superior data management and data mining capabilities along with effective project management features that include organizing, assigning and monitoring projects at different levels.SilicoCyte™ suppor…

The TaqMan® Custom Array is a 384-well micro fluidic card that enables a researcher to perform 384 simultaneous real-time PCR reactions without the need to use liquid-handling robots or multichannel pipettors to load. This low- to medium- throughput array allows for 1-8 samples to be run in parallel against 12-384 TaqMan® Gene Expression Assay targets that are pre-loaded into each of the wells on the array. The TaqMan Custom…

TaqMan® Express Plates are a cost-effective and convenient 96-well format complimenting Applied Biosystem's line of individual TaqMan® Gene Expression Assays, and pre-spotted 384-well TaqMan® Arrays. Customize any of the six available plate formats with dried-down TaqMan® Assays by positioning each in specific well locations. This flexibility enables an efficient means to screen biological samples for the presence and amount…