Products & Reviews

The Human Regulatory T Cell Whole Blood Staining Kit contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3 cells in whole blood samples. The Anti-Human Foxp3 PE (PCH101) monoclonal antibody reacts with the amino terminus of human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead/winged-helix family of transcription factors.…

These bacterial expression vectors carry the gene for either GFP (wild type Aequorea victoria green fluorescent protein) or GFPuv (a GFP variant optimized for maximal fluorescence when excited by UV light [360–400 nm]) and are driven by the lac promoter. These vectors can also be used as a source of the indicated fluorescent protein coding sequences. The fluorescent protein gene can be amplified by PCR or readily excised using…

RNA GEM is a simple, rapid RNA extraction solution that leverages a powerful thermophilic proteinase to simultaneously lyse cells and eliminate RNases, thereby stabilizing the RNA and maintaining its integrity without harsh chemicals or multiple washes.  The single-tube extraction protects precious samples, ensuring all RNA is preserved. It is the ideal solution for gene expression from a very small number of cells without t…

The SatisFection reagent is a novel cationic polymer suitable for both transient and stable transfections in the presence or absence of serum. This polymer functions by binding and condensing DNA into a polyplex that enters the cell via endocytosis.  Cost savings of up to 70% per transfection  Unmatched performance in some hard-to-transfect lines  Maximum expression 36 - 72 hours post-transfection  Ideal reagent for exp…

The SatisFection reagent is a novel cationic polymer suitable for both transient and stable transfections in the presence or absence of serum. This polymer functions by binding and condensing DNA into a polyplex that enters the cell via endocytosis.  Cost savings of up to 70% per transfection  Unmatched performance in some hard-to-transfect lines  Maximum expression 36 - 72 hours post-transfection  Ideal reagent for exp…

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.  CMV promoter for high-level protein expression in mammalian cells  Large multiple cloning site contains 15 unique sites  Clone PCR products generated with any PCR e…

Ribonuclease R (RNase R) from E. coli is a magnesium-dependent 3ʹ to 5ʹ exoribonuclease available for circular RNA cleanup, alternative splicing research, and gene expression studies.  Key features:  Efficacy and quality assured under ISO 13485 certification Available in bulk and for custom configurations Effectively degrades linear RNA, Y-structure RNAs, but not lariat loops or circular RNAs Digests linear RNAs…

The CHEMICON® Alpha/Beta Integrin-Mediated Cell Adhesion Array kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Integrin-Mediated Cell Adhesion Array can be completed within 1-2 hours, providing a rapid, quantitative assay for the characterization of alpha…

Chemicon's CXCR4 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal tools for screening for antagonists of interactions between CXCR4 and SDF-1a. Radiolabeled SDF-1a binds to the CXCR4 membranes with a Kd of 0.9 nM. With 0.04-0.13 nM 125I-labeled SDF-1a, an approximately 3-fold window of specific bin…

Chemicon's CX3CR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of fractalkine/CX3CR1 interactions. The membrane preparations exhibit a Kd of 0.05 nM for [125I]-fractalkine. With 2.5 mg/well CX3CR1 Membrane Prep and 0.05 nM [125I]-fractalkine, a greater tha…

Chemicon's CRF1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CRF1 interactions with its ligands. The membrane preparations exhibit a Kd of 1 nM for [125I]-sauvagine. With 2.5 μg/well CRF1 Membrane Prep and 0.5 nM [125I]-sauvagine, a greater than 12-fold…

Chemicon's CRTH2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of PGD2/CRTH2 interactions. The membrane preparations exhibit a Kd of 10 nM for [3H]-Prostaglandin D2. With 2.5 μg/well CRTH2 Membrane Prep and 10 nM [3H]-Prostaglandin D2, a greater than 5-fold…

Chemicon's VPAC1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of VPAC1 interactions with VIP. The membrane preparations exhibit a Kd of 1.2 nM for [125I]-VIP. With 2.5 μg/well VPAC1 Membrane Prep and 0.4 nM [125I]-VIP, a greater than 5-fold signal-to-backg…

Chemicon's Y2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of Y2 interactions and its ligands. The membrane preparations exhibit a Kd of 1.28 nM for [125I]-human PYY. With 0.5 nM [125I]-human PYY, 5 or 10µg/well Y2 Membrane Prep yields greater than 10-fold…

Chemicon's VPAC2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of VPAC2 interactions with PACAP. The membrane preparations exhibit a Kd of 0.9 nM for [125I]-PACAP27. With 5 µg/well VPAC2 Membrane Prep and 0.5 nM [125I]-PACAP27, a greater than 15-fold signal…

Chemicon's α1A membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of α1A interactions with prazosin. The membrane preparations exhibit a Kd of 1.34 nM for [3H]-prazosin. With 5 µg/well α1A Membrane Prep and 1 nM [3H]-prazosin, a greater than 7-fold signal-to-bac…

Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of κ interactions with diprenorphine. The membrane preparations exhibit a Kd of 2.3-3.7 nM for [3H]-diprenorphine. With 10 µg/well κ Membrane Prep and 2.5 nM [3H]-diprenorphine, a greater than 3-fold…

Chemicon's D3 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of D3 interactions and its ligands. The membrane preparations exhibit a Kd of 0.1 nM for [125I]-Iodospiperone. With 5.0ug/well D3 membrane prep and 0.1 nM [125I]-Iodospiperone, a greater than 4-fol…

EMD Millipore's 5-HT1B membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of 5-HT1B. The membrane preparations exhibit a Kd of 10.5 nM for [3H]-GR125743. With 7 nM [3H]-GR125743, 10μg/well 5-HT1B Membrane Prep typically yields greater than 8-fold si…

EMD Millipore's GIP membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at GIP Receptor. The membrane preparations exhibit a Kd of 0.44 nM for [125I]-GIP. With 0.2 nM [125I]-GIP, 10 µg/well GIP Receptor Membrane Prep typically yields greater than 8-f…

Chemicon's CXCR7 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at CXCR7. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-SDF-1α. With 0.25 nM [125I]-SDF-1α, 10µg/well CXCR7 Membrane Prep typically yields greater than 4-fold signal-…

EMD Millipore’s cloned human GLP-1 Receptor -expressing cell line is made in the CHO host, which supports optimal levels of recombinant GLP-1 Receptor expression for robust agonist-induced cAMP signal. In addition, the cell line contains optimized levels of a proprietary promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists…

EMD Millipore's S1P1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P1 interaction with its ligands. The cell line exhibits a calcium response with EC50s of 141 μM for Sphingosine 1-phosphate. The membrane preparations exhibit EC50s of 9.5 μM for Sphingosine 1-phospha…

The BluePrint Molecular Subtyping Profile combined with the patient's MammaPrint test result, provides a greater level of clinical information to assist in therapeutic decision-making. BluePrint is an 80-gene expression signature which classifies breast cancer into Basal-type, Luminal-type and ERBB2-type cancers. An increasing body of evidence in the medical community suggests that breast cancer patients within these molecular…

The Cell Stimulation Cocktail (500X), a cocktail of phorbol 12-myristate 13-acetate (PMA) and ionomycin is sufficient to induce activation of many cell types to produce cytokines. This cocktail can be used to induce cytokine production in in vitro cell culture for subsequent detection in immunoassays. It can also be used in conjunction with the Protein Transport Inhibitor Cocktail (500X) (cat. 00-4980) for intracellular retent…