Products & Reviews

Chemicon's cloned human S1P1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant S1P1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P1 and its ligands.

Chemicon's cloned human PK2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PK2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PK2 and its ligands.

EMD Millipore’s cloned human Ghrelin Receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Ghrelin Receptor expression on the cell surface and contains high levels of the promiscuous G protein to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at Ghrelin Receptor.

EMD Millipore's cloned human S1P5-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant S1P5 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P5 and its ligands.

The NEBNext Quick DNA Library Prep Master Mix Set for 454 contains enzymes and buffers in convenient master mix formulations that are ideally suited for sample preparation for next-generation sequencing, and for preparation of expression libraries. NEBNext® reagents are a series of highly pure reagents that facilitate sample preparation of DNA or RNA for downstream applications such as next generation sequencing and expressio…

Increased amplification – Up to 5,000-fold Greater sensitivity due to increased yields of full-length cDNA GeneChip® quantities of aRNA from only 100 ng input RNA Efficient labeling produces high percent present calls and low 3'/5' ratios Expression profiling using gene arrays to simultaneously measure the expression levels of thousands of genes in a single experiment has become the most widely used method for assessing…

Support transient transfection, growth, and expression of a variety of recombinant proteins in HEK293 cells with Thermo Scientific™ HyCell TransFx-H Media, a high-performining, animal-derived component-free cell culture media. HyCell TransFx-H media is a hydrolysate-free and regulatory-friendly Thermo Scientific ADCF™ cell culture media. Its versatility allows quick adaptation and supports excellent transfection, high viable…

Support transient transfection, growth, and expression of a variety of recombinant proteins in CHO cells with Thermo Scientific™ HyCell TransFx-C Media, a high-performing, animal-derived component-free cell culture media. HyCell TransFx-C media is a hydrolysate-free and regulatory-friendly Thermo Scientific ADCF™ cell culture media. Its versatility allows quick adaptation and supports excellent transfection, high viable cell…

High-quality total RNA for human, mouse, and rat microarray gene-expression profiling that acts as a consistent control for standard data set comparisons. Comprised of 10 cell lines for human, 11 for mouse, and 14 for rat giving broad gene coverage UHRR selected as one of the two standards in the FDA led Microarray Quality Control (MAQC) project   For Research Use Only. Not for Use in Diagnostic Procedures.

The CHEMICON® Alpha Integrin-Mediated Cell Adhesion Array kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Integrin-Mediated Cell Adhesion Array can be completed within 1-2 hours, providing a rapid, quantitative assay for the characterization of alpha inte…

The CHEMICON® Beta Integrin-Mediated Cell Adhesion Array kit is designed as a cost effective, efficient method for identification of cell surface integrins. The kit format uses 12 x 8-well removable strips in a plate frame for convenience and flexibility in designing assays. The Integrin-Mediated Cell Adhesion Array can be completed within 1-2 hours, providing a rapid, quantitative assay for the characterization of beta integr…

Vascular Endothelial Cell Growth Factor (VEGF) is a homodimeric protein secreted by a variety of vascularized tissues. The reported activities of VEGF include stimulation of endothelial cell growth, angiogenesis, and capillary permeability. Chemicon's mouse VEGF is a 39 kDa homodimeric protein consisting of two 165 amino acid polypeptide chains comprising the full length mouse VEGF164 sequence and an N-terminal methionine resi…

EMD Millipore's CB2 membrane preparations are crude membrane preparations made from stable recombinant cell lines with a high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists of CB2. The membrane preparations exhibit a Kd of 2.7 nM for [3H]-CP55,940. With 5 μg/well CB2 Membrane Prep and 2.5 nM [3H]-CP55,940, greater than 10-fold signal-to-background ratio is obtained.

EMD Millipore's cloned human MC2-MRAP double stable cell line is made in the Chem-1 host, which supports high levels of recombinant MC2 expression on the cell surface and contains high levels of the endogenous promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between MC2 and its ligands.

Chemicon's δ membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists at δ. The membrane preparations exhibit a Kd of 1.12 nM for [3H]-DADLE. With 0.9 nM [3H]-DADLE, 10 µg/well δ Membrane Prep typically yields greater than 4-fold signal-to-background rati…

Chemicon's cloned human Glucagon Receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Glucagon Receptor expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between the Glucagon Receptor and its ligands…

EMD Millipore's EP2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of EP2. The membrane preparations exhibit a Kd of 14.52 nM for [3H]-PGE2. With 7.5 nM [3H]-PGE2, 10µg/well EP2 Membrane Prep typically yields greater than 3-fold signal-to-backgr…

The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Based on the T7 promoter-driven system, Novagen’s pET System has been used to express thousands of different proteins. pET Vectors are supplied as purified plasmid DNA (10 µg) plus an Induction Control strain (supplied as a glycerol stock). Complete sequences and detailed vector maps are available.

Overnight Express™ Instant TB Medium is a complete granulated culture medium for high-level protein production without the need to monitor cell growth in the pET and other IPTG-inducible bacterial expression systems. Cell mass and target protein yield are often increased several-fold as compared with induction using IPTG. The method is based on media components that are metabolized differentially to promote growth to high dens…

The Human Regulatory T Cell Whole Blood Staining Kit contains the buffers and monoclonal antibody necessary to successfully stain and identify Foxp3 cells in whole blood samples. The Anti-Human Foxp3 PE (PCH101) monoclonal antibody reacts with the amino terminus of human Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2. Foxp3 is a 49-55 kDa protein and a member of the forkhead/winged-helix family of transcription factors.…

These bacterial expression vectors carry the gene for either GFP (wild type Aequorea victoria green fluorescent protein) or GFPuv (a GFP variant optimized for maximal fluorescence when excited by UV light [360–400 nm]) and are driven by the lac promoter. These vectors can also be used as a source of the indicated fluorescent protein coding sequences. The fluorescent protein gene can be amplified by PCR or readily excised using…

RNA GEM is a simple, rapid RNA extraction solution that leverages a powerful thermophilic proteinase to simultaneously lyse cells and eliminate RNases, thereby stabilizing the RNA and maintaining its integrity without harsh chemicals or multiple washes.  The single-tube extraction protects precious samples, ensuring all RNA is preserved. It is the ideal solution for gene expression from a very small number of cells without t…

The SatisFection reagent is a novel cationic polymer suitable for both transient and stable transfections in the presence or absence of serum. This polymer functions by binding and condensing DNA into a polyplex that enters the cell via endocytosis.  Cost savings of up to 70% per transfection  Unmatched performance in some hard-to-transfect lines  Maximum expression 36 - 72 hours post-transfection  Ideal reagent for exp…

The SatisFection reagent is a novel cationic polymer suitable for both transient and stable transfections in the presence or absence of serum. This polymer functions by binding and condensing DNA into a polyplex that enters the cell via endocytosis.  Cost savings of up to 70% per transfection  Unmatched performance in some hard-to-transfect lines  Maximum expression 36 - 72 hours post-transfection  Ideal reagent for exp…

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.  CMV promoter for high-level protein expression in mammalian cells  Large multiple cloning site contains 15 unique sites  Clone PCR products generated with any PCR e…