UHPLC and HPLC Products & Reviews

The ZORBAX NH2 can be utilized for normal-phase and weak anion-exchange compounds and can separate vitamins, carbohydrates and sugars in the reversed-phase mode. The NH2 consists of a propyl-amino silane phase bonded to ZORBAX SIL. For normal-phase chromatography, the ZORBAX product line offers a choice of bonded and non-bonded silica packings. ZORBAX NH2 Features: Amino-propyl silane phase bonded to ZORBAX SIL Used for…

The ZORBAX RX-SIL is made from very pure porous silica microspheres and is useful for very hydrophilic compounds with high organic mobile phases. With low acidity and metal content the ZORBAX Rx-SIL is ideal for normal-phase separation of polar compounds that exhibit poor peak symmetry on more acidic silica. The ZORBAX Rx-SIL is also stronger than other silica types and is less acidic than the ZORBAX-SIL. ZORBAX Rx-SIL Featu…

ResiPore columns are the ideal choice for the analysis of resins, and condensation polymers with complex molecular weight distributions that include oligomer content. By combining a low 3 µm particle size and high pore volume, high efficiency ResiPore columns offer maximum resolution of these intermediate molecular weight polymers. Features: Efficient separation of complex molecular weight distributions Reveals oligome…

Agilent AdvanceBio Glycan Mapping Columns are designed and manufactured to deliver FAST, high resolution, reproducible glycan identification using HILIC chromatography.

To successfully separate trityl-off, deprotected oligonucleotides, you need columns that have high resolving power and are robust enough to withstand challenging conditions.

The Affinity Removal LC Column - Human Albumin is designed to chromatographically remove the most interfering high-abundance protein (albumin) from human samples (e.g., plasma, serum, urine, or cerebrospinal fluid). They are compatible with most FPLC or HPLC pumps or instruments, including Agilent 1200/1100 instruments.

Agilent AdvanceBio SEC columns ensure easy, fast, and accurate quantitation for mAb aggregation and fragment analysis. AdvanceBio SEC offers two different particle sizes, 2.7 and 1.9 µm.

Agilent Bio SEC-3 HPLC columns are a breakthrough technology for size exclusion chromatography (SEC). They are packed with spherical, narrowly dispersed 3 µm silica particles coated with a proprietary hydrophilic layer.

Agilent Bio SEC-5 HPLC columns are packed with 5 µm silica particles coated with a proprietary, neutral, hydrophilic layer for maximum efficiency and stability. Our specially designed packing also promotes high pore volume, improving both peak capacity and resolution. 

Method validation is a critical step for biotherapeutic drug development. It ensures that the analytical method is suitable for its intended purpose, and that it meets regulatory requirements.

Agilent ZORBAX GF-250 and GF-450 size exclusion (gel filtration) columns are for size separations of proteins and other biomolecules. The separation range is 4,000-900,000 for globular proteins when using GF-250 and GF-450 columns in series.

Agilent AdvanceBio MS Spent Media columns are HILIC columns that deliver fast, sensitive, and reproducible separations of underivatized amino acids and other polar metabolites found in bioprocessor cell culture media for mass spectrometric detection.

Agilent Bio Ion-Exchange HPLC columns are packed with polymeric, nonporous, ion-exchange particles. Bio Ion-Exchange Columns are designed for high resolution, high recovery and highly efficient separations of peptides, oligonucleotides and proteins.

Thorough characterization of monoclonal antibodies includes the identification and monitoring of acidic and basic isoforms. The Agilent Bio MAb HPLC columns feature a unique resin specifically designed for high-resolution charge-based separations of monoclonal antibodies.

PL-SAX for Biomolecules is ideal for the anion-exchange HPLC separations of proteins and deprotected synthetic oligonucleotides under denaturing conditions.

PL-SCX is a macroporous PS/DVB matrix with a very hydrophilic coating and strong cation-exchange functionality. 

ZORBAX Ion Exchange columns are available as both Strong Anion-Exchange (SAX) and Strong Cation-Exchange (300SCX) columns. 

Agilent 2-AA (formerly ProZyme) labeled N-glycan standards and libraries are commonly used as qualitative standards for LC/FLD and LC/MS. 

Agilent 2-AB (formerly ProZyme) labeled N-glycan standards and libraries are commonly used as qualitative standards for LC/FLD and LC/MS. 

Agilent 2-AB (formerly ProZyme) labeled O-glycan standards are commonly used as qualitative standards for LC/FLD and LC/MS.

Agilent APTS (formerly ProZyme) labeled glycan standards are commonly used for qualitative N-glycan identification or as migration standards for capillary electrophoresis with laser-induced fluorescence (CE/LIF).

Agilent Blood Group Oligosaccharides (formerly ProZyme) standards are offered as disaccharide and trisaccharide forms.

Agilent Glycan Alignment (formerly ProZyme) Standard Set includes the Gly-Q glucose unit (GU) ladder and Gly-Q migration standards.

Agilent N-glycan standards (formerly ProZyme) and libraries are used in many analytical procedures including LC/FLD, LC/MS, and CE/LIF.

Agilent InstantAB (formerly ProZyme) labeled N-glycan standards and libraries are commonly used as qualitative standards for LC/FLD and LC/MS.