Proteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.
OxyBlot™ Protein Standard is composed of five proteins (phosphorylase B, BSA, ovalbumin, carbonic anhydrase, trypsin inhibitor) containing 1-3 dinitrophenylhydrazone (DNP) residues per protein. These attached DNP moieties permit the visualization of the proteins by the detection system of the OxyBlot™ Protein Oxidation Detection Kit. OxyBlot™ Protein Standard serves as an internal control for the electrophoresis, western trans…
Streptavidin isolated from Streptomyces avidinii covalently bound to agarose.
This kit allows for quick and reproducible immunoprecipitation (IP) by using a spin column. The system is more reproducible than regular IP's, which are problematic with regards to washing the protein A/G agarose without disrupting the agarose bed. The binding of the antibody/antigen complex in Catch and Release® is reversible, and elution of the immune complex can occur with native or denaturing buffers. The system has been…
The IHC Select® Immunoperoxidase Secondary Detection System is intended for use with the CHEMICON IHC Select® Prediluted Primary Antibody reagents, which contain rabbit and mouse IgG for the qualitative identification of antigens by light microscopy in paraffin-embedded tissues. The IHC Select® Immunoperoxidase Secondary Detection System may also be used with other rabbit or mouse primary antibodies. The IHC Select® Immunopero…
Upstate's new Nitrotyrosine Assay Kit, Chemiluminescence Detection is a competitive ELISA for the quantitation of tyrosine nitration. The kit includes all reagents needed for measurement of tyrosine nitration, including white high binding 96-well plates, nitrated BSA standard, a Nitrotyrosine antibody, LumiGLO® chemiluminescent detection substrate, and wash buffers. The assay has a wide dynamic range and high precision, making…
Upstate®'s cGMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic GMP (cGMP, Guanosine 3', 5'-cyclic monophosphate) from extracts, lysates or supernatants of cells of any species. The kit comprises a specific anti-cGMP antibody which recognizes all species, an alkaline phosphatase (AP)-labeled cGMP conjugate, two 96 well microplates pre-coated with ananti-Ra…
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The AKT plate is coated with a specific mouse monoclonal anti-AKT capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are…
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Akt plate is coated with a specific mouse monoclonal Akt capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are incub…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The EGFR plate is coated with a specific mouse monoclonal anti-EGFR capture antibody. Sample lysate or the standard included in the kit are incubated in the microwells allowing EGFR…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The EGFR plate is coated with a specific mouse monoclonal EGFR capture antibody. Sample lysate or the standard included in the kit are incubated in the microwells allowing EGFR antig…
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Src plate is coated with a specific mouse monoclonal anti-Src capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are…
The UPSTATE colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The Met plate is coated with a specific mouse monoclonal anti-Met capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit are…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The PRAS40 plate is coated with a specific mouse monoclonal PRAS40 capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit ar…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The PRAS40 plate is coated with a specific mouse monoclonal PRAS40 capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standard included in the kit ar…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The FAK plate is coated with a specific mouse monoclonal FAK capture antibody. Sample lysate or the standard included in the kit are incubated in the microwells allowing FAK antigen…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The IR plate is coated with a specific mouse monoclonal IR (β subunit) capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standards included in the…
The UPSTATE® colorimetric STAR (Signal Transduction Assay Reaction) ELISA kit is a solid phase sandwich enzyme linked immunosorbent assay that provides a fast, sensitive method to detect specific levels of signaling targets in whole cell extracts. The IR plate is coated with a specific mouse monoclonal IR (β subunit) capture antibody on the microwells of the 96-well clear plate. Sample lysate or the standards included in the k…
Upstate®'s cAMP HTS Immunoassay kit is a competitive immunoassay for highly sensitive and rapid chemiluminescent quantitation of cyclic AMP from cell extracts of any species. The kit comprises a specific anti-cAMP antibody that recognizes all species, an alkaline phosphatase (AP)-labeled cAMP conjugate, ten 96 well microplates pre-coated with an anti-Rabbit antibody, cAMP standard, and an Alkaline Phosphatase chemiluminescence…
The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity t…
The UPSTATE Non-Radioactive Universal EZ-TFA Transcription Factor Assay provides a fast, sensitive method to detect specific transcription factor DNA binding activity in whole cell or nuclear extracts. This assay combines the principle of the electrophoretic mobility shift assay (EMSA) with the 96-well based enzyme-linked immunosorbent assay (ELISA), enabling manual or high-throughput sample analysis with greater sensitivity t…
The CHEMICON ssDNA Apoptosis ELISA Kit is a convenient, sensitive method for early detection of apoptosis. The assay can be performed in two formats: 1. Cells are grown, treated with apoptosis inducing agents, stained and analyzed in the same microtiter plate. This assay is suitable for high throughput screening when induction of apoptosis is used as the endpoint of drug activity. 2. Cell suspensions obtained from cultures or…
The rat C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures rat CRP.
The human C-Reactive Protein (CRP) kit is a double polyclonal antibody sandwich enzyme immunoassay (EIA), which measures human CRP.
With the ChemiKine assay system, sheep polyclonal antibodies generated against human NT-3 are coated onto a microplate and are used to capture NT-3 from a sample. NT-3 specific, biotin conjugated, mouse monoclonal antibodies detect the captured NT-3. After addition of streptavidin-enzyme, substrate and stop solution the amount of NT-3 is determined. The standard curve demonstrates a direct relationship between Optical Density…
