Enzymes > Nuclease Products & Reviews

Lentiviral CRISPR-Cas9 components for robust gene editing in biologically relevant cell types

Point, click, edit. Guaranteed.

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Expand your options for genome editing with the Alt-R CRISPR-Cas12a System.

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant PURedit Cas9 protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments

Recombinant Cas9 Plus protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

Recombinant Cas9-GFP protein from Streptococcus pyogenes is a ready-to-use reagent for genome engineering experiments.

The Transcreener dAMP Exonuclease Assay directly measures dAMP produced by exonuclease enzymes as they cleave polynucleotides. 

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Recombinant, high-purity endonucleases for genome editing experiments

Thermo Scientific Exonuclease I digests single-stranded DNA 3' to 5' (requires Mg2+ and a free 3'-OH). The nuclease is inactivated by heating at 80°C for 15 minutes.

Thermo Scientific Shrimp Nuclease is an endonuclease that cleaves phosphodiester linkages in DNA to yield di- and oligonucleotides with 5’-phosphate and 3’-hydroxyl termini. The enzyme has a high preference for double-stranded DNA, and can be used to selectively degrade dsDNA, leaving ssDNA and RNA intact. The enzyme is inactivated after 15 minutes at 65 °C.

Thermo Scientific RNase A (ribonuclease A) is supplied as a chromatographically pure freeze-dried powder, salt-free for easy reconstitution in most buffers.

Thermo Scientific Ribonuclease H (RNase H) is an endonuclease that degrades the RNA portion of DNA-RNA hybrids. It hydrolyses phosphodiester bonds of RNA in RNA:DNA heteroduplexes. It produces products with 3’-OH and 5’-phosphate. RNase H will not degrade single or double-stranded DNA or RNA.

Finnzymes’ novel PowerCut™ Dicer produces a pool of 25–27 nucleotide-long siRNA molecules from any dsRNA. This efficient endoribonuclease originating from Giardia intestinalis outperforms all other siRNA producing enzymes by cleaving the substrate with 100 % efficiency yet leaving the resulting siRNA intact. The high quality siRNA produced is ideal for gene silencing studies, for example. Pooled siRNAs are also preferred over…