Gene Expression and Molecular Cloning Products & Reviews

Agilent provides a range of Interference-resistant Helper Phages that enable ssDNA recovery from phagemid vectors. The pBlueScript II Phagemid Vector systems are available in R408 and VCSM13 format. R408 Interference-Resistant Helper Phages are stable and easy to grow, and are provided in a single-strand size of ~4 KB.

Helper phages for mass excision of Lambda ZAP, ZAP Express libraries, and for single strand rescue protocols.  Use for mass excision of Lambda ZAP®, and ZAP Express® libraries  Interference-resistant helper phage  Eliminates the possibility of co-infection from helper phage

Agilent miRNA qPCR Detection Reagents are high-specificity reagents for the polyadenylation of microRNAs (miRNAs) from total RNA. The miRNA qPCR Detection Reagents also enable the synthesis of first-strand cDNA from the tailed miRNAs.

The pBluescript II phagemids (plasmids with a phage origin) are cloning vectors designed to simplify commonly used cloning and sequencing procedures, including the construction of nested deletions for DNA sequencing, generation of RNA transcripts in vitro and site-specific mutagenesis and gene mapping. The pBluescript II phagemids have an extensive polylinker with 21 unique restriction enzyme recognition sites.  New cDNA synt…

pCMV-Script PCR cloning system is a polymerase chain reaction (PCR) cloning method that can be performed in 1-hour without adding bases to the primers. The kit permits the efficient cloning of PCR fragments with a high yield and a low rate of false positives.  CMV promoter for high-level protein expression in mammalian cells  Large multiple cloning site contains 15 unique sites  Clone PCR products generated with any PCR e…

Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.

Agilent mammalian transfection kits provide all the necessary reagents to efficiently and rapidly conduct DNA transfection in mammalian cells. It offers a simplified protocol that achieves high efficiency transfections in a variety of cell lines.

The MBS Mammalian Transfection Kit increases transfection efficiencies by up to 100-fold. Cells are incubated with CaPO4/DNA coprecipitate for only 3 hours compared to 6 to 12 hours with traditional CaPO4 methods. 

The In Situ beta-Galactosidase Staining Kit provides an easy and rapid method to determine transfection efficiency by indicating beta-Galactosidase activity in individual intact cells.  In situ staining of cells directly in dishes without removing or manipulating cells  Rapid detection of Ÿ-gal activity in transfected cells  Results in 24 hours  Maximum signal with low background  Deep blue staining reaction  Kit in…

Our single-stranded DNA binding standard is a helix-destabilizing E. coli protein. The 75.6-kD protein is essential for replication, recombination, and repair processes.

The HybriZAP-2.1 two-hybrid vector system, a eukaryotic system to detect proteinprotein interactions in vivo, provides a method for the rapid identification of genes encoding proteins that interact with a bait protein. The system is based on the ability to separate eukaryotic transcriptional activators into two separate domains, the DNA binding domain (BD) and the transcriptional activation domain (AD).  Detects protein-prote…

The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.  Tightly controlled T7 RNA polymerase-based gene expression in     E.coli    High-level expression upon induction  Convenien…

The pET E.coli expression system is a widely used in vivo bacterial expression system due to the strong selectivity of the bacteriophage T7 RNA polymerase for its cognate promoter sequences, the high level of activity of the polymerase, and the high efficiency of translation mediated by the T7 gene.  Tightly controlled T7 RNA polymerase-based gene expression in     E.coli    High-level expression upon induction  Convenien…

The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

The InterPlay adenoviral TAP System combines our unique tandem affinity purification (TAP) system with our exclusive adenoviral gene delivery system, the AdEasy Adenoviral Vector System for enhanced gene delivery to a broader range of mammalian cells, higher protein yields, and improved purification and analysis of endogenous interacting protein partners.

The LacSwitch II inducible mammalian expression system utilizes an improved vector system in which several elements of the lac operon have been modified for use in eukaryotic cells for the control of gene expression. The establishment of a highly regulated expression system in eukaryotic cells is an invaluable tool for the study of cell cycle, oncogenicity, cytogenicity, and the mechanisms of gene regulation.

The Luciferase Assay Kit provides rapid, sensitive, and quantitative measurement of American firefly (Photinus pyralis) luciferase activity in cultured mammalian cells.  Sensitive, widely used reporter assay  Rapid, quantitative results  Reporter activity immediately available upon translation  Useful in conjunction with PathDetect systems

The pDual and pDual GC expression vectors are designed for high-level expression of heterologous genes in both eukaryotic and prokaryotic systems.High-level protein expression in bacterial or mammalian systems Saves time by eliminating the need to subclone Express protein as a native or tagged fusion protein Epitope tagged for simple detection and affinity purification Clone seamlessly without compatible restriction sites Avai…

SureSelect Cancer CGP assay offers comprehensive genomic profiling for solid tumors. This pan-cancer NGS panel enables detection of somatic variants, including single nucleotide variants (SNVs), copy number variants (CNVs), insertions/deletions (indels), translocations (TLs), gene fusions, and the immuno-oncology biomarkers TMB (tumor mutational burden) and MSI (microsatellite instability).

The Agilent SurePrint G3 Human GE 8x60K Microarrays offer the same high sensitivity and accuracy provided by the earlier generation arrays but adds the benefits of extended coverage, greater throughput, and increased cost savings. The new SurePrint G3 Human GE 8x60K Microarrays and Human GE 4x44K v2 Microarrays are based on updated transcriptome databases for mRNA targets, while the SurePrint G3 arrays also include probes for…

Achieve ultra-high RNA yields with MEGAscript™ kits—ideal for in vitro or in vivo translation studies needing unlabeled or low specific activity RNA.

FDA approved Modern cellular and molecular biology poses the strictest requirements for the usage of sensitive samples such as living cells, nucleic acids or other cell components. With this ambition of quality in mind, Eppendorf offers a large selection of microcapillaries for the most varied applications. VacuTips capillaries and TransferTips® for intracytoplasmatic sperm injection (ICSI ) TransferTip (ES) for embryo…

Predesigned probe assays for gene expression analysis

Modulation of gene expression with modified oligos

Unleash the full power of a well-designed library. With Twist Bioscience, you are assured that the library you design contains the specific modifications, exactly where you want them, saving you valuable resources for the downstream screening process.