PCR and Thermal Cycling Products & Reviews

Real-time fluorescent DNA detection

Streamline your PCR workflows by consolidating protocols and reaction conditions, increase pass rates, amplify difficult templates without bias, and increase sensitivity – all with a single enzyme. The second-generation KAPA2G Robust DNA Polymerase was evolved to solve - achieve consistent amplification across a broad range of amplicon types (both GC and AT-rich). The versatility and robustness of the polymerase allows for con…

The PrimeScript RT-PCR Kit is a two-step RT-PCR kit featuring PrimeScript RTase, an RNase H Minus reverse transcriptase enzyme that offers robust reverse transcription of virtually any RNA template. This RT-PCR kit is conveniently configured to include all reagents needed for two-step endpoint RT-PCR, including primers, PrimeScript RTase, and PCR polymerase enzyme. PrimeScript RTase is an MMLV Reverse Transcriptase and enable…

The EmeraldAmp MAX PCR Master Mix ensures PCR specificity and includes an optimized buffer, PCR enzyme, dNTP mixture, gel loading dye (green) and a density reagent in a convenient 2X premix format. Only primer and DNA template need to be added to initiate the reaction. This premix can be used for routine PCR applications as well as those requiring PCR specificity. Completed reactions are capable of being analyzed directly via…

Tube strips consist of eight 0.2 mL tubes connected together Cap strips are sold separately Made of thin wall polypropylene and designed for precise fit in heat blocks for optimum heat transfer Certified DNase- and RNase-free Autoclavable at 121°C

Axygen® 32 well clear PCR microplate is designed to fit standard 96 well blocks. Ultra-thin and consistent wall thickness allows precise thermal transfer for optimum results. Can be sealed with 8 strip caps or sealing film.RNase-/DNase-freeNonpyrogenicNonsterileManufactured with 99.9% pure virgin polypropylene

KAPA PROBE FORCE is a highly inhibitor resistant qPCR master mix that removes the need for DNA purification, enabling streamlined sample-to-Cq workflows. The master mix contains a third generation DNA polymerase evolved to overcome blood, tissue, and plant PCR inhibitors. Crude samples can now be analyzed with comparable accuracy, reproducibility, and sensitivity as purified DNA using KAPA PROBE FORCE.   Features:…

The PCRmax Eco 48 utilises patented hollow liquid filled silver and gold block and optical technologies to deliver extreme speed, sensitivity and data quality, with optimised 40 cycle protocols completing in as little as 15 minutes, all using standard plastic ware and consumables. This matched up with sensitivity down to 1 copy detection and the world most thermally accurate block the Eco 48 will deliver Speed. Confidence. Per…

Ambion® Cells-to-CT™ Kits allow you to measure relative gene expression by real-time RT-PCR without having to purify RNA prior to amplification. Cells-to-CT™ Kits are available for both TaqMan® and SYBR® Green detection. Cells-to-CT™ kits provide a complete workflow for real-time qRT-PCR analysis directly from cultured cells without RNA purification. Featuring a unique method for lysing cultured cells while removing genomic…

Ambion® mMESSAGE mMACHINE® High Yield Capped RNA Transcription Kits use Ambion's patented high-yield transcription technology for the routine synthesis of 15–35 µg of 7-methyl guanosine capped RNA from 1 µg of template DNA. Each kit includes sufficient reagents for 25 reactions. Features: High yields of capped RNA—up to 39 µg of RNA per reaction  Extra convenience—cap analog-nucleotide mix provided  Simplified rea…

The OneStep qMethyl™ is a single step, real-time PCR procedure for bisulfite-free determination of DNA methylation status.

Ready-to-use, easy interpretation of results, Highly-sensitive, no need for internal control

Complete solution for automated PCR

Fast, uniform results from a thermal cycler that fits easily onto your deck

The SimpliAmp™ Thermal Cycler is a small, easy-to-use, and accurate thermal cycler that suits any lab's needs for everyday PCR. It is crafted with the reliable engineering and performance you’ve come to expect from Applied Biosystem® instruments. It is elegantly designed to deliver quiet cycling, and features an innovative, safe heated lid and a large LCD color touch screen. Key Features and Benefits: VeriFlex™ blocks…

Thermo Scientific™ DreamTaq™ Hot Start PCR Master Mix is a ready-to-use 2X reaction mix that includes DreamTaq™ Hot Start DNA Polymerase, DreamTaq buffer, magnesium, and dNTPs. The master mix allows easy reaction setup with fewer pipetting steps, with only primers, template, and water needing to be added. A version of this master mix is also available with pre-added density reagent and electrophoresis tracking dyes. DreamTa…

istretto (1x 32) constitute a complete range of PCR machines. Originally called peqSTAR, these machines manufactured in Germany by Peqlab integrated in 2014 to the VWR group, are now part of the brand VWR ® Collection.  All thermal cyclers are certified "Appli'Ozyme PCR". As part of this certification, Ozyme ensures that embedded machines and software meet the most demanding standards on the market and work optimally for a…

BHQ® (Black Hole Quencher®)-labeled Scorpions primers for PCR analysis are unlike dual-labeled probes and Molecular Beacons because they combine primer and probe in one molecule, with the primer at the 3' end and the probe contained within a hairpin-loop structure at the 5' end. Scorpions primers utilize a uni-molecular mechanism that acts faster in solution for instantaneous fluorescence in real-time PCR.  Similar to Molec…

Thermo Scientific ThermoPrime Taq DNA Polymerase with 10X ReddyMix PCR Buffer. This unique buffer has an inert red dye and a density reagent added. After thermal cycling, a sample of the PCR mix may be removed and loaded directly onto an agarose gel without the addition of loading dye. ReddyMix has no adverse effect on the outcome of PCR; yields are the same as with standard ThermoPrime Taq DNA Polymerase. Convenient Master Mi…

Rapid high-throughput, plate-based real-time PCR amplification and detection instrument.

Advance your research with increased application versatility

High-fidelity PCR reagents featuring the thermostable iProof Polymerase. This enzyme utilizes Sso7d fusion polymerase technology to provide highly accurate amplification of targets up to 37 kb.

Complete PCR reagent with standard Taq DNA Polymerase and inert dye

Intuitive and easy to use for all levels of experience. Now you can get up and running quickly with the Applied Biosystems QuantStudio 3 Real-Time PCR System, a simple and affordable real-time PCR solution designed for users with all levels of experience. Get connected to your data easier than before with a real-time PCR systems that offers an interactive touch screen and the option to choose Web browser-based software enabled…

TaqMan Universal Master Mix II brings the quality and reliability you’ve come to expect from Applied Biosystems to an improved master mix formulation for real-time PCR.