Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
Ambion Silencer® In Vivo Ready siRNAs undergo the additional purification and testing necessary for introduction of siRNA into animals. After HPLC purification and annealing, each siRNA is dialyzed to remove salt, sterile filtered, and endotoxin tested. All Silencer Pre-Designed and Validated siRNAs are available in this format, as are Custom Synthesized siRNAs.
Abmion Validated GAPDH Positive Control siRNA and non-targeting Negative Control siRNAs are available for human, mouse and rat with the same chemical modifications for enhanced efficacy as in other Ambion Silencer Select siRNAs.
For the first time, you can obtain the benefits of Ambion Silencer Select siRNAs in a convenient 96- or 384-well plate format, plated to your specifications. These siRNA collections enable medium- and high-throughput analysis of phenotypes resulting from inhibition of sets of targets from specific functional classes or your favorite list of targets.
Silencer siRNA Libraries are sets of Ambion-designed siRNAs targeting all of the genes in a genome or a particular gene class, making them ideal for pathway analysis and target identification/validation. Most libraries include three individual siRNAs per gene for enhanced data reliability. Full sequence information is provided with each library.
Applied Biosystems currently offers five TaqMan Copy Number Assays to detect copy number variation. Copy number is an important polymorphism in the human genome associated with genetic diseases such as cancer, immune diseases, and neurological disorders. TaqMan Copy Number Assays were designed to detect CYP2D6, CYP2A6, CYP2E1, GSTT1, and GSTM1.
The TaqMan® Custom Array is a 384-well micro fluidic card that enables a researcher to perform 384 simultaneous real-time PCR reactions without the need to use liquid-handling robots or multichannel pipettors to load. This low- to medium- throughput array allows for 1-8 samples to be run in parallel against 12-384 TaqMan® Gene Expression Assay targets that are pre-loaded into each of the wells on the array. The TaqMan Custom…
The TaqMan Custom Plating service offers the convenience of pre-plated TaqMan® Gene Expression Assays, Custom Assays, and Custom Probe/Primer Sets in 96- or 384-well plates. This cost-effective custom plating service gives you the flexibility and reliability you need for your screening applications. Whether you are looking for biomarkers, testing for toxicity, searching for microbes, or performing other high-throughput scree…
Applied Biosystems TaqMan Drug Metabolism Genotyping Assays are available for basic research and clinical research. Genotype over 2,600 high value polymorphisms located in regulatory elements and coding regions for 220 drug metabolism and transporter genes with TaqMan® Assay technology.
TaqMan® Express Plates are a cost-effective and convenient 96-well format complimenting Applied Biosystem's line of individual TaqMan® Gene Expression Assays, and pre-spotted 384-well TaqMan® Arrays. Customize any of the six available plate formats with dried-down TaqMan® Assays by positioning each in specific well locations. This flexibility enables an efficient means to screen biological samples for the presence and amount…
TaqMan® Gene Sets are pre-defined lists of genes grouped by pathway or disease target class which can be ordered separately, as a custom plate, or configured directly onto TaqMan® Arrays to enable you to perform hundreds of real-time PCR reactions simultaneously on the Applied Biosystems 7900HT Fast Real-Time PCR System. Simple to implement, TaqMan Gene Sets are customizable, and when placed on a TaqMan Array, require mini…
TaqMan® Gene Signature Arrays enable analysis of hundreds of TaqMan® Gene Expression Assays on a 384-well micro fluidic card with minimal effort. TaqMan Gene Signature Arrays are readily available and delivered 2–4 days after ordering. They are packaged conveniently with two or four arrays per pack, making them cost effective, and just the right size for any experiment. Choose from pathway-specific pre-defined arrays such…
TaqMan® MicroRNA Assays quantitate miRNAs with the specificity and sensitivity of TaqMan® Assay chemistry. A simple, two-step protocol requires only reverse transcription with a miRNA-specific primer, followed by real-time PCR with TaqMan® probes. The assays target only mature miRNAs, not their precursors, ensuring biologically relevant results. Get gold standard TaqMan Assay performance for miRNA quantitation and profiling…
The TaqMan® MicroRNA Reverse Transcription Kit provides the necessary components for optimal performance using Applied Biosystems TaqMan® MicroRNA Assays. Components of this kit are used with the RT primer provided with the TaqMan MicroRNA Assay to convert miRNA to cDNA.
TaqMan® PreAmp Pools are pre-pooled sets of Applied Biosystems TaqMan® Gene Expression Assays ready for use with four specific TaqMan® Gene Signature Arrays. TaqMan PreAmp Pools are designed for challenging RNA samples, such as very small quantities, or degraded material and genes that have low expression levels. Use PreAmp Pools to increase the quantity of your sample targets without introducing bias. Four pools are availab…
The TaqMan Sample-to-SNP Kit provides a streamlined protocol for performing TaqMan® chemistry-based genotyping analysis from any sample with a single kit. The kit is comprised of two parts: the DNA Extract All Lysis Reagents and the TaqMan® GTXpress™ Master Mix. The DNA Extract All Lysis Reagents reduce prolonged procedures for the release of real-time PCR-ready DNA to a 5 minute protocol. They can process a wide variety of…
The Iso-Gold kit is a rapid-format isotyping kit that reduces the labor of the traditional ELISA kit from a day-and-a-half procedure to a five-minute assay. It determines the isotype of your monoclonal antibody, assess the purity of your purified monoclonal antibody*, verify the purity of your purchased monclonal antibody* and reduces runtime. *multiple lines appear and indicate subclass contamination
Developed for your most demanding LC/MS/MS quantitative applications, the Quattro Premier™ XE tandem quadrupole mass spectrometer features the highest levels of sensitivity, selectivity, robustness, speed and accuracy. To maximize the analytical advantages of your ACQUITY UPLC® System, the Quattro Premier XE operates at the highest data acquisition rates. Together, the ACQUITY UPLC® System and the Quattro Premier XE give you i…
The mTRAQ™ Reagents are duplex amine-specific, stable isotope labeled reagents designed for high confidence protein and peptide biomarker verification using MRM analysis on the QTRAP® system and the triple quadrupole mass spectrometers. * Duplex mass difference tags (Non-Isobaric Amine Tags) * Established chemistry with > 95% labeling efficiency * High selectivity MRM based relative quantitation of proteins/peptides/PTMs * Sup…
SimGlycan™ Software (a product of PREMIER Biosoft International) predicts the structure of a glycan from the MS/MS data acquired by AB SCIEX mass spectrometers. It facilitates the study of glycosylation and post translational modifications. The software works directly with *.t2d and *.wiff data generated from AB SCIEX instruments, including the 4800 Plus MALDI TOF/TOF™ Analyzer, 4000 QTRAP® System, and QSTAR® Elite System. * M…
Osteoprotegerin (OPG) EIA is an enzyme immunoassay intended for the quantitative determination of OPG in plasma, serum or cell culture supernatants. OPG is a dimeric glycoprotein of the tumor necrosis factor (TNF) receptor family that acts as a soluble receptor for sRANKL (soluble receptor-activated NF-kB ligand) and prevents it from binding to and activating RANK, thus inhibiting osteoclast development. Possible indications f…
The Ampli-sRANKL EIA is an enzyme immunoassay intended for the quantitative determination of human free soluble RANK-Ligand in plasma, serum and cell culture supernatants. Format:ELISA / EIA, Sample Type:Serum, Plasma, Cell Culture, Sample Size:100 µl, Size:96 Wells, Range:0.125 - 2 pmol/l, Sensitivity:0.02 pmol/l, Incubation Time:Overnight, 1 hour 30 minutes
Oxidized LDL (Ab) EIA is a enzyme immunoassay intended for the quantitative determination of human IgG autoantibodies to oxidized low density lipoprotein (LDL) in serum. Format:ELISA / EIA, Sample Type:Serum, Sample Size:50 µl, Size:96 Wells, Range:37 - 1200 mU/ml, Sensitivity:27 mU/ml, Incubation Time:2 hours 20 minutes
The LPL (Lipoprotein Lipase) ELISA is an enzyme immunoassay intended for the quantitative determination of human LPL in serum or plasma. LPL is an enzyme that cleaves triglycerides for fatty acid uptake in the periphery. Determination of the LPL concentration might be a complementary measure for LPL activity. Format:ELISA / EIA, Sample Type:Serum, plasma, Sample Size:50 µl, Size:96 Wells, Range:9 - 500 ng/mL, Sensitivity:9 n…
The DKK-1 EIA is an enzyme immunoassay intended for the quantitative determination of human DKK-1 in serum. Format:ELISA / EIA, Sample Type:Serum, Sample Size:15 µl, Size:96 Wells, Range:3.125 - 50 pmol/L, Sensitivity:0.38 pmol/L, Incubation Time:Overnight, 1 hour 30 minutes
Axygen X-Gal IPTG spray is a convenient ready-to-use solution of optimal quantities of IPTG, X-Gal and stabilizers presented in a spray bottle for easy, accurate and consistent screening of Blue/White colonies. Advantages of Axygen X-Gal-IPTG Spray include:• Easy, accurate and convenient. • Sprays deliver optimal concentration and volume consistently for consistent and accurate results. • No need to weigh X-Gal and IPTG and…
