Products, services, reviews and techniques used in the identification and validation of the disease-causing target genes and proteins.
Chromobodies® - Your perfect reagents for cellular research including High-Content Analysis. Chromobodies are fluorescent nanoprobes suitable for real time analyses of endogenous cellular structures and processes in live cells. Chromobodies® are a novel species of extremely small intracellular antibodies. They are based on the antigen binding domain (VHH) derived from heavy chain antibodies of camelids genetically fused to a f…
Fusion of proteins to Glutathione S-Transferase (GST) of Schistosoma japonicum is a common technique to increase solubility and expression level of a protein. Furthermore, GST-tagged fusion proteins are often used in protein-protein interaction studies. Both approaches require highly specific tools to isolate and detect GST fusion proteins. GST-Trap The GST-Trap is a highly specific and robust GST-binding protein coupled to ma…
DNA methylation was the first epigenetic modification identified and has been intensively studied for more than half a century. By now it is clear that Dnmt1, the major eukaryotic DNA methyltransferase, faithfully maintains genome-wide methylation patterns and plays an essential role in the epigenetic network controlling gene expression and genome stability during development. However, the molecular mechanisms that ultimately…
Proliferating cell nuclear antigen (PCNA) is a homotrimeric ring-shaped protein that encircles DNA and acts as a loading platform for multiple, transiently interacting factors participating in various DNA transactions. PCNA plays a key role in DNA replication, repair, cell cycle regulation, and post-replicative transactions like DNA methylation and chromatin remodeling. All these cellular processes are regulated by a complex n…
Being able to visualize endogenous cellular structures in live cells is key for many high content analyses. The two Chromobodies® of the ChromoTek’s Cell Organelle Toolbox offer a new way to investigate key compartments of the cell non-invasively. Lamin-Chromobody® The new Lamin-Chromobody® is a major breakthrough especially in High-Content Analysis (HCA) as apoptosis assays thus far rely on static endpoint assays. The nucle…
Inductively Coupled Plasma Mass Spectrometer (ICP-MS) The Science of sensitivity Bruker's ICP-MS is famous for its innovative and unique technology and has now been further refined to meet the ever increasing demands of latest application and laboratory requirements. Bruker's aurora M90 continues to be the workhorse for the routine laboratory, now with additional and improved characteristics. In addition to the aurora M90, Bru…
Disposable cuvettes for spectrophotometry, optical PS visible range cuvettes, UV Grade PMMA cuvettes. Standard and semi-micro disposable cuvettes for spectroscopy made of optical PS and PMMA UV grade optical windows to achieve optimal transmittance through spectral range from 340 to 800 nm (OPS) an from 280 to 800 nm (PMMA UV qrade). The engineering and quality control determine the reproducibility of the cuvettes. These are s…
The VenaT4™ biochip model combines Cellix's proprietary microfluidic flow technology for cell recruitment under shear stress with traditional transwell chamber models. Used to investigate and quantify cell recruitment to various ligands in the presence of chemoattractants contained within the microwells mimicking tissue and / or injury sites. The biochips contain 4 microcapillaries in parallel, each with an embedded microporo…
The FlowCellect™ Human NK Cell Characterization Kit includes three directly conjugated antibodies, Anti-CD3-APC, Anti-CD56-PE, and Anti-CD16 FITC. This three color kit is designed to characterize NK within mixed cell populations. The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 three-colo…
DART® Ion Mobility Spectrometer The new DART® Ion Mobility Spectrometer is a completely integrated benchtop instrument equipped with Resistive Glass technology and improved performance. It provides DART-IMS analysis of solid, liquid or gas analytes quickly and easily for use in homeland security, pharmaceutical or laboratory applications. Sample preparation is easy, and identifiable results can be seen in less than 10s. The…
Identify causal variants from human sequencing data in just hours Rapidly Identify and Prioritize Variants Ingenuity Variant Analysis combines analytical tools and integrated content to help you rapidly identify and prioritize variants by drilling down to a small, targeted subset of compelling variants based both upon published biological evidence and your own knowledge of disease biology. Biological Interpretation of your Var…
VERSA 1100 SPE Workstation Automated solid phase extraction (SPE) is an increasingly useful technique designed for rapid sample preparation and purification for chromatographic or other analyses. Automated solid phase extraction (SPE) is a chromatographic separation technique utilized to remove solid or semi-solid compounds from a mixture in solution. Samples are concentrated and purified enabling downstream use by MS, GC, LC-…
SmartFlare Probes work by recognizing specific native RNA sequences within living cells, therefore there are many possible sequences that you could design to recognize your target of interest. Instead of lysed cells, switch to live cells. And while you’re at it, eliminate sample preparation and transfection steps all together. Live cell RNA detection is now possible, in a single incubation step using inert nanoparticle techno…
cOmplete His-Tag Purification Resin is an innovative high-capacity IMAC (Immobilized Metal Affinity Chromatography) matrix for convenient single-step purification of His-tagged proteins from lysates. Roche's new proprietary nickel-chelate chemistry is compatible with commonly used reducing agents (such as DTT), chelating metalloprotease inhibitors (such as EDTA), and a wide range of buffer substances and salt conditions. The w…
The labelling of DNA samples used in array comparative genomic hybridisation (aCGH) is a critical step in the experimental process as poor labelling can result in inaccurate data. OGT’s CytoSure Genomic DNA Labelling Kits have been uniquely developed and optimised to enable rapid delivery of high quality results with high signal-to-noise ratios.
OnTarget™ is a powerful, new platform for detection of rare tumor mutations in tissue or plasma. • Detect 100 or more mutations in a single assay, without sample splitting • Quantify mutations present at 0.01% allele frequency and above • Enrich mutations extracted from plasma or FFPE in about 1 hour
Introducing an all-in-one centrifugal device for the protein purification workflow.Traditional protein purification is a long process with many steps and multiple devices, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. No matter what your workfl…
Reliable detection of copy number changes and loss of heterozygosity on a single array for haematological malignancies and solid cancers. The CytoSure Cancer +SNP array delivers: ■ Flexible choice of reference sample ■ Confident detection of CNV and LOH on a single array ■ An optimised design allowing identification of key genomic aberrations ■ Cancer-specific tracks enabling fast and easy data generation and interpretation o…
The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC enzymes. Unlike conventional assays that are dependent upon a s…
Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Panel 1 (Cat. No. 48-617MAG) is a premixed panel to be used for the simultaneous quantification of the following 4 analytes in cell lysates: c-Myc, Nanog, Oct 3/4, and Sox2. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay. Backgro…
Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Kit 2 (Cat. No. 48-620MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates: E-Cadherin, EpCam, KLF4, LIN-28, and the glycostructures TRA-1-60, TRA-1-81 and SSEA1. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has suffic…
Cryo Express (CX) “dry shippers” are designed to safely transport a variety of materials at cryogenic temperatures. The unique absorbent material prevents a liquid spill if the unit is tipped over. Storage temperature inside the shipping cavity remains at approximately -190˚C until the liquid nitrogen evaporates from the absorbent material. CX & CXR Series Features: Advanced Concept Absorbent enables faster charging Co…
The Extended Time Series (XT) Series of cryogenic refrigerators are designed for long-term storage of a variety of materials at cryogenic temperatures. This series also offers low profile models (XTL), with 5” canisters. XT Series Features: New Rugged Construction – ribbed high strength aluminum body, magniformed necktube design, and more durable paint Versatile storage system – convenient canister numbered index location…
Rocket 4D Synergy is designed primarily to evaporate large sample volumes, with autofeed if required, allowing automatic evaporation of volumes up to 100 litres. Rocket 4D Synergy provides fast, safe evaporation for solvents with a boiling point of up to 165°C (DMF) and can easily dry aqueous samples. The system feeds the solution for evaporation to the evaporation vessel, controls temperature and pressure, prevents bumping…
The Magna ChIP HiSens kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating…
