Products, services, reviews and techniques used in lead molecule delivery, formulation and toxicology, using assays and animal models.
XLfit® is a powerful curve-fitting and data analysis package that integrates completely with Microsoft® Excel. XLfit offers an extensive selection of data analysis and calculation tools, including a superior range of fit and statistics models as well as charts and graphs to visualize, interpret and present experimental data, all in Excel.XLfit works within the familiar Excel spreadsheet environment to enable curve fitting an…
With the all new CLC RNA Workbench, CLC bio brings you complex analysis of RNA molecules in a fully integrated, user-friendly and graphically advanced bioinformatics framework. Advanced The CLC RNA Workbench gives the user easy access to a range of complex algorithms and options. RNA secondary structures can be predicted using state-of-the-art free energy minimization algorithms and parameters, thermodynamic and statistical d…
The new Guava Nexin® reagent monitors induction of apoptosis quickly and easily, with minimal chance for contamination. Annexin V is a calcium-dependent phospholipid binding protein with high affinity for phosphatidylserine (PS), a membrane component normally localized to the internal face of the cell membrane. Early in the apoptotic pathway, molecules of PS are translocated to the outer surface of the cell membrane where Ann…
Fluorescein-conjugated anticoagulant for the detection of phosphatidylserine in the outer leaflet of apoptotic cellsProduct Description Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system. Sample material: Cell lines and freshly isolated cells. Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and sho…
Biotin-conjugated anticoagulant for the detection of phosphatidylserine in the outer leaflet of apoptotic cellsProduct Description Detection: Annexin-V-Biotin can be used in immunohistochemistry with a secondary detection system (e.g., Streptavidin-POD conjugate). Sample material: Cell lines and freshly isolated cells Specificity: Annexin-V binds in a calcium-dependent manner to negatively charged phospholipid surfaces, and…
Alexa 568-conjugated anticoagulant for the detection of phosphatidylserine on the outer leaflet of apoptotic cellsBenefits Detection flexibility: Analysis of results with flow cytometry and/or fluorescence microscopy. Alexa is a red dye, which can be used for double or triple staining with other fluorescent markers (e.g., fluorescein-labeled surface markers). Accuracy: Accurately distinguish apoptotic cells from necrotic…
Kit for the detection and quantification of apoptosis and differentiation from necrosis at the single-cell level, based on Annexin-V-labelingProduct Description Detection: Annexin-V-FLUOS can be directly detected in FACS analysis and immunochemistry without a secondary detection system. Sample material: Cell lines and freshly isolated cells. Specificity: Annexin-V-FLUOS binds in a Ca2+-dependent manner to negatively charged ph…
Kit for the detection and quantification of apoptotic cell death on a single-cell level by light microscopy in immunocyto- and immunohistochemistry Benefits Sensitive: The maximum intensity of labeling (cell staining) of apoptotic cells is higher than the nick translation method. Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction, but before the addition of the secondary d…
Kit for the detection and quantification of apoptotic cell death on a single-cell level by flow cytometry and fluorescence microscopy, and for double labeling with fluorescein-labeled cell markers (TMR red)Benefits Convenient: No secondary detection system required. Accurate: Identification of apoptosis at a molecular level (DNA-strand breaks) and identification of cells at the very early stages of apoptosis. Flexible:…
Kit for the detection and quantification of apoptosis (programmed cell death) at the single-cell level, based on labeling of DNA strand breaks (TUNEL technology): Analysis by fluorescence microscopy or flow cytometry Benefits Sensitive: The direct labeling procedure using fluorescein-dUTP reduces background labeling. Fast: The use of fluorescein-dUTP allows analysis of the samples directly after the TUNEL reaction. Co…
ApplicationThe kit is used for the quantitative determination of BrdU incorporated into cellular DNA using a 96-well microplate cell ELISA format. Benefits Safer: No radioisotopes are used. Accurate: The results strongly correlate to those obtained with the [3H]-thymidine method. Sensitive: As sensitive as [3H]-thymidine. Fast: The use of multiwell ELISA readers allows for processing of a large number of samples. Ea…
Application The Cell Proliferation ELISA, BrdU (chemiluminescent) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple nonradioactive alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell…
Application The Cell Proliferation ELISA, BrdU (colorimetric) belongs to the second, improved generation of kits for measuring DNA synthesis. It is designed as a precise, fast, and simple colorimetric alternative to quantitate cell proliferation based on the measurement of BrdU incorporation during DNA synthesis in replicating (cycling) cells. Thus, the Cell Proliferation ELISA can be used in many different in vitro cell syst…
ApplicationThe In Situ Cell Proliferation Kit, FLUOS belongs to the second generation of improved kits for the detection of DNA synthesis at the single-cell level (in situ) by immunocyto-/immunohistochemistry. The kit can be used in many different in vitro and in vivo cell systems, for example: Detection and quantification of cells in S-phase Determination of growth fractions for tumors in animal-model systems Detectio…
ApplicationThe immunoreactivity of the M30 antibody is confined to the cytoplasm of apoptotic cells. It can be easily used for: Immunocytochemistry Flow Cytometry Immunohistochemistry: paraffin-embedded tissue sections (secondary enhancer reagents needed for this particular application) and cryostat sections Benefits Determine caspase activity, even in formalin-embedded tissue. Identify the stage of apoptosis (…
ApplicationSamples suitable for the Caspase 3 Activity Assay are: Lysates of apoptosis-induced cells Recombinant caspase 3 (CPP32) The Caspase 3 Activity Assay can be used to: Detect and quantify in vitro caspase 3 activity in cell lysates Analyze caspase 1, 2, 3, or 7 activity by simply omitting the capture-antibody step Perform inhibitor studies for Caspase 3 This kit is intended as a tool for life science…
ApplicationDuring apoptosis, the activation of specific proteases, called Caspases (cysteinyl-aspartic-acid-proteases), is one of the initial intracellular biochemical events. The Homogeneous Caspases Assay can be used to detect caspase activity in life science research applications: for sensitive quantification of activated caspases for screening of caspase inhibitors as an automatable one-step assay for high-throughp…
Quantitative determination of Hu-IFN alpha A, 2, A/D, D, K and 4b in cell culture and media Sample Volume: 100 µl Catalog Number: 41100-2 Quantity: 5 x 96 Assay Range: 12.5-500 pg/ml, 156-5000 pg/ml Shipping Conditons: Wet Ice Storage Temp.: 2-8˚C Preservative: Kathon Shelf Life: 12 months from date of manufacture 2007 Retail price (Effective 10/1/2007): $1,530
Quantitative determination of Hu-IFN alpha A, 2, A/D, B2, C, D, G, H, I, J1, K, 1, 4b and WA in cell culture and media Sample Volume: 100 µl Catalog Number: 41105-2 Quantity: 5 x 96 Assay Range: 12.5-500 pg/ml, 156-5000 pg/ml Shipping Conditons: Wet Ice Storage Temp.: 2-8˚C Preservative: Kathon Shelf Life: 12 months from date of manufacture 2007 Retail price (Effective 10/1/2007): $2,135
Quantitative determination of Hu-IFN alpha A, 2, A/D, B2, C, D, G, H, I, J1, K, 1, 4b and WA in cell culture and media Sample Volume: 100 µl Catalog Number: 41110-1 Quantity: 1 x 96 Assay Range: 12.5-500 pg/ml, 156-5000 pg/ml Shipping Conditons: Wet Ice Storage Temp.: 2-8˚C Preservative: Kathon Shelf Life: 12 months from date of manufacture 2007 Retail price (Effective 10/1/2007): $550
Quantitative determination of Hu-IFN alpha A, 2, A/D, B2, C, D, G, H, I, J1, K, 1, 4b and WA in cell culture and media Sample Volume: 100 µl Catalog Number: 41110-2 Quantity: 5 x 96 Assay Range: 12.5-500 pg/ml, 156-5000 pg/ml Shipping Conditons: Wet Ice Storage Temp.: 2-8˚C Preservative: Kathon Shelf Life: 12 months from date of manufacture 2007 Retail price (Effective 10/1/2007): $2,135
Quantitative determination of Hu-IFN Omega in cell culture, media, serum and plasma Sample Volume: 100 µl Catalog Number: 41395-1 Quantity: 1 x 96 Assay Range: 3-500 pg/ml Shipping Conditons: Wet Ice Storage Temp.: 2-8˚C Preservative: N/A Shelf Life: 18 months from date of manufacture 2007 Retail price (Effective 10/1/2007): $630
Quantitative determination of Hu-IFN Beta in cell culture and media Sample Volume: 100 µl Catalog Number: 41410-2 Quantity: 5 x 96 Assay Range: 25-2000 pg/ml Shipping Conditons: Wet ice/ Dry Ice Storage Temp.: 2-8˚C/ -20˚C Preservative: Kathon Shelf Life: 12 months from date of manufacture 2007 Retail price (Effective 10/1/2007): $1,995
Quantitative determination of Hu-IFN Gamma in cell culture and media Sample Volume: 100 µl Catalog Number: 41500-1 Quantity: 1 x 96 Assay Range: 12.5-500 pg/ml Shipping Conditons: Wet Ice Storage Temp.: 2-8˚C Preservative: 0.01% Thimerasol Shelf Life: 6 months from date of manufacture 2007 Retail price (Effective 10/1/2007): $300
Quantitative determination of Mu-IFN Alpha in cell culture and media Sample Volume: 100 µl Catalog Number: 42100-1 Quantity: 1 x 96 Assay Range: 12.5-500 pg/ml Shipping Conditons: Wet Ice Storage Temp.: 2-8˚C Preservative: 0.01% Thimerasol Shelf Life: 6 months from date of manufacture 2007 Retail price (Effective 10/1/2007): $560
