Identification and optimization of the chemical, series of chemicals or bio-molecule that will interact with the target using impartial, expert opinion about the best equipment, services and the latest techniques
EMD Millipore’s Ready-to-Assay GPCR frozen cells are designed for simple, rapid calcium assays with no requirement for intensive cell culturing. EMD Millipore has optimized the freezing conditions to provide cells with high viability and functionality post-thaw. The user simply thaws the cells and resuspends them in media, dispenses cell suspension into assay plates and, following over night recovery, assays for calcium respon…
Chemicon's cloned human CCR7-expressing cell line is made in the Chem-2 host, which supports high levels of recombinant CCR7 expression on the cell surface and contains high levels of the promiscuous G protein Galpha16 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR7 and its ligands.
Chemicon's cloned human CX3CR1 -expressing cell line is made in the Chem-4 host, which supports high levels of recombinant CX3CR1 expression on the cell surface and contains high levels of promiscuous G proteins to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CX3CR1 and its ligands.
Chemicon's CX3CR1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of fractalkine/CX3CR1 interactions. The membrane preparations exhibit a Kd of 0.05 nM for [125I]-fractalkine. With 2.5 mg/well CX3CR1 Membrane Prep and 0.05 nM [125I]-fractalkine, a greater tha…
Chemicon's cloned human C5a receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant C5aR expression on the cell surface and contains high levels of the promiscuous G protein Ga15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of C5a/C5aR interactions.
Chemicon's cloned human C5a receptor membrane preparations are ideal tools for screening for antagonists of C5a/C5aR interactions. The C5aR Membrane Preps bind specifically to 125I-labeled ligand C5a, with Kd of 0.59 nM. With 5 μg membrane per well and 0.1 nM 125I-labeled C5a, more than 15-fold signal:background was obtained.
Chemicon's membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of FPR1 and its ligands. The membrane preparations exhibit a Kd of 0.39 nM for [125I]-WKYMVm. With 5 μg/well FPR1 Membrane Prep and 0.5 nM [125I]-WKYMVm, a greater than 20-fold signal-to-background is…
Chemicon's cloned human CB1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CB1 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of CB1.
Chemicon's CB1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists at CB1. The membrane preparations exhibit a Kd of 7 nM for [3H]-SR141716A. In the presence of 2 nM [3H]-SR141716A with CP55940 as unlabeled competitor, 5 μg/well and 10 μg/well CB1 M…
Chemicon's cloned human CB2-expressing cell line is made in the Chem-4 host, which supports high levels of recombinant CB2 expression on the cell surface and contains high levels of promiscuous G proteins to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CB2 and its ligands.
EMD Millipore's CB2 membrane preparations are crude membrane preparations made from stable recombinant cell lines with a high-level of GPCR surface expression; thus, they are ideal HTS tools for screening for agonists and antagonists of CB2. The membrane preparations exhibit a Kd of 2.7 nM for [3H]-CP55,940. With 5 μg/well CB2 Membrane Prep and 2.5 nM [3H]-CP55,940, greater than 10-fold signal-to-background ratio is obtained.
EMD Millipore's cloned human MC2-MRAP double stable cell line is made in the Chem-1 host, which supports high levels of recombinant MC2 expression on the cell surface and contains high levels of the endogenous promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between MC2 and its ligands.
Chemicon's cloned human CRF1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CXCR2 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CRF1 and its ligands.
Chemicon's CRF1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CRF1 interactions with its ligands. The membrane preparations exhibit a Kd of 1 nM for [125I]-sauvagine. With 2.5 μg/well CRF1 Membrane Prep and 0.5 nM [125I]-sauvagine, a greater than 12-fold…
Chemicon’s cloned MCHR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant MCHR1 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between MCHR1 and its ligands.
EMD Millipore’s cloned human sst2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant sst2 expression on the cell surface and contains optimal levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between sst2 and its ligands.
Chemicon's CRTH2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of PGD2/CRTH2 interactions. The membrane preparations exhibit a Kd of 10 nM for [3H]-Prostaglandin D2. With 2.5 μg/well CRTH2 Membrane Prep and 10 nM [3H]-Prostaglandin D2, a greater than 5-fold…
Chemicon's cloned human GPR54-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR54 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists of GPR54.
Chemicon's GPR14 Membrane Preparations are ideal tools for screening for antagonists of urotensin II/GPR14 interactions. The membrane preparations exhibit a Kd of 0.4 nM for [125I]-urotensin II. With 10 mg/well GPR14 Membrane Prep and 0.3 nM [125I]-urotensin II, a greater than 20-fold signal-to-background ratio is obtained.
Chemicon's cloned human NTR1-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant NTR1 expression on the cell surface and contains high levels of the promiscuous G protein Galpha15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between NTR1 and neurotensin.
Chemicon's cloned human CCR9-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant CCR9 expression on the cell surface and contains high levels of the promiscuous Galpha15 protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between CCR9 and its ligands.
Chemicon's CCR9 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCR9 interactions with its ligands. The membrane preparations exhibit an EC50 of 8 nM for TECK/CCL25 in a GTPgammaS binding assay.
Chemicon's PAR2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of PAR2 interactions with its ligands. The membrane preparations exhibit an EC50 of 1.9 μM for furoyl-LIGRLO in a GTPγS binding assay.
Chemicon's cloned human D2L-expressing cell line is made in the Chem-7 host, which supports high levels of recombinant D2 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between D2 and its ligands.
EMD Millipore’s cloned human D2L-expressing cell line is made in the CHO host, which supports optimal levels of recombinant D2L expression for robust agonist-induced cAMP signal. Thus, the cell line is an ideal tool for screening for agonists and antagonists at the D2L Receptor.
