To uncover the crucial information for criminal investigations and legal proceedings, forensic analysts rely on sophisticated methodologies that yield objective and scientifically grounded insights. These approaches include DNA analysis, fingerprinting, ballistics, and toxicology. Explore how robust forensic analysis empowers law enforcement agencies and legal professionals to solve crimes and uphold justice.
AccuPower® HotStart PCR PreMix is a PCR master mix containing a thermostable DNA polymerase, thermostable pyrophosphatase, reaction buffer, dNTPs, tracking dye, and patented stabilizer in a ready to use hotstart PCR master mix.Bioneer uses a unique enzyme-mediated HotStart PCR that provides robust and reliable results. Bioneer’s Top DNA Polymerase is inhibited at lower temperatures (
AccuPower® CycleScript RT (available with: dT20, dN12, or dN6 primers) PreMix is an easy to resuspend lyophilized PCR Master Mix of CycleScript Reverse Transcriptase, a primer, and all of the other components for cDNA synthesis conveniently packaged in individual tubes. Simply add template RNA, and water. Then, the reverse transcription is performed by either a Cyclic RT reaction (patent pending) or conventional reverse transc…
The AccuPower® RT PreMix is a master mix for cDNA synthesis that consists of an easy to resuspend, lyophilized mix of M-MLV Reverse Transcriptase, RNase inhibitor, dNTPs, reaction buffer, tracking dye, and patented stabilizer. The master mix kit is used for first strand cDNA synthesis from RNA. Downstream applications include cDNA amplification with reverse transcription PCR. All of the key components are premixed at optimal c…
AccuPower® RT-PCR PreMix contains all the components necessary for sequential cDNA synthesis and amplification in one tube (one-step RT-PCR). This RT-PCR PreMix consists of both M-MLV Reverse Transcriptase, RNA dependent DNA polymerase, and a thermostable DNA polymerase in a lyophilized mix of dNTPs, reaction buffer, RNase inhibitor, tracking dye, and stabilizer. The kit can be used for double stranded cDNA synthesis from low…
Bioneer’s AccuPrep® Plasmid MiniPrep DNA Extraction Kit allows for the extraction of highly-pure plasmid DNA from as E. coli bacterial cultures. A260/A280 ratio greater than 1.8 and Yields of up to 20 μg of high-copy plasmid DNA can be routinely attained using the AccuPrep Plasmid MiniPrep DNA Extraction Kit. The AccuPrep Plasmid MiniPrep DNA Extraction Kit was developed for plasmid DNA purification from cultured bacterial cel…
Bioneer’s AccuPrep® PCR Purification Kit allows rapid purification of DNA fragments (dsDNA and ssDNA) from PCR products and other enzymatic reactions. Purification of up to 10 µg of DNA fragments from PCR and other enzymatic products within 5 minutes with > 90% recovery are routine with the AccuPrep PCR Purification Kit. Size range is 100 bp - 10 kb, so common 20 - 40 mer oligonucleotides are easily removed. Elution volume can…
Bioneer’s AccuPrep® Gel Purification Kit allows rapid DNA gel purification of up to 10 μg DNA fragments or plasmid DNA from low-melting, TAE and TBE agarose gels as well as PCR products and enzymatic reaction mixtures. Fragments 70 bp – 10 kb can be isolated in less than 15 minutes with the AccuPrep Gel Purification Kit Recovery efficiencies are QC’ed to be greater than 70%. Elution volume can be as little as 30 μl when concen…
Bioneer’s AccuPrep® GMO DNA Extraction Kit allows rapid DNA extraction from agricultural and food products to detect genetically modified organisms (GMOs). The AccuPrep GMO DNA Extraction Kit yields large amounts of high-purity DNA; for example, 20~40 μg of plant DNA for 1 g of powdered bean, with an average A260/A280 ratio greater than 1.8. AccuPrep GMO DNA Extraction Kit works on the principle that DNA, In the presence of ch…
Bioneer’s AccuPrep® Stool DNA Extraction Kit allows for the rapid extraction of genomic, bacterial, viral, and parasite DNA from fresh or frozen stool. The AccuPrep Stool DNA Extraction Kit yields large amounts of contaminant free, high quality DNA routinely with average A260/A280 ratio greater than 1.8.The AccuPrep Stool DNA Extraction Kit quickly and conveniently extracts up to 5μg of DNA from 100~200 mg of stool. In the pre…
Bioneer’s AccuPrep® Viral RNA Extraction Kit is the ideal solution for rapid extraction of viral RNA from cell-free samples such as serum, plasma, CSF, urine, and from a variety of RNA virus, such as HIV, HAV, HCV and enteroviruses. The viral RNA extracted with the AccuPrep Viral RNA Extraction Kit can be directly used as a template for RT or RT-PCR. AccuPrep Viral RNA Extraction Kit obtained has an average A260/A280 ratio gre…
Bioneer’s AccuPrep® 96 Plasmid DNA Extraction Kit is used for the high throughput extraction of plasmid DNA from 96 different bacterial culture samples. Bacterial cultures can be grown and lysed in the AccuPrep 96-well blocks provided with the Plasmid Extraction kit. Purification is accomplished with the BioVac 96 Vacuum Manifold. Average A260/A280 ratio greater than 1.8. Maximum yield of 17 μg.The DNA extraction protocol used…
Bioneer’s AccuPrep® 96 Genomic DNA Extraction Kit allows for the simultaneous genomic dna purification from 96 samples of cultured cells, whole blood, buffy coat, lymphocytes, plasma, serum, and other biological fluids. Yield is sample dependent, but is typically 6 μg per 200 µl healthy whole human blood. The AccuPrep 96 Genomic DNA Extraction Kit is based on the principle of DNA binding to a silica membrane in the presence of…
Bioneer’s AccuPrep® 96 PCR Purification Kit is a high throughput PCR purification kit that allows PCR product purification from up to 96 PCR reactions at once. The AccuPrep 96 PCR Purification Kit allows purification of up 10 μg of DNA fragments from PCR and other enzymatic products within 25 minutes. The AccuPrep 96 PCR Purification Kit features recovery efficiencies up to 95%. Size range is 100 bp - 10 kb, thus 20-40 mer oli…
Designed for use with the Bioneer AccuPrep® 96-well plate series - handles 96 samples simultaneously for high throughput DNA extraction. Simply attach to a vacuum pump for easy processing of all AccuPrep 96-well plate DNA extraction kits.
The Tissue RNA PreMate™ kit is designed for total RNA extraction from tissue, cultured cells, leukocytes or serum. The PreMate protocol is based on a Modified guanidinium salt-lysis method to optimally extract total RNA. Extracted total RNA is treated with PreMate’s RNase-free DNase to eliminate any genomic DNA. The RNA Isolated with the Tissue RNA PreMate kit is suitable for Northern analysis, dot blot hybridization, poly A s…
Viral RNA PreMate contains all of the required reagents for easy isolation of total RNA from RNA viruses such as HCV using the guanidinium salt-lysis method. Possible sources include tissue, cultured cells, leukocytes, and serum. The extracted RNA can be used for molecular cloning, RT-PCR, Northern analysis, dot blot hybridization, poly-A (+) selection, RNase protection assays, and other application that require pure RNA. Vira…
DNA PrepMate™ - M is used to extract genomic DNA for PCR from microorganisms with tough cell walls (such as Mycobacterium tuberculosis complex and Mycoplasma pneumoniae) using a standard microwave oven. DNA preparation is accomplished quickly and efficiently. Source materials can be sputum, CSF, urine, pleural fluid or tissue.
DNA PrepMate™ II uses an optimal binding buffer and silica matrix to effectively isolate DNA from bacterial cells, PCR products, gel fragments and enzymatic reaction mixtures. Examples include the purification of DNA fragments or plasmid DNA from low-melting, TAE and TBE agarose gel, high salt solutions, and lysed bacterial cells (using alkaline lysis). DNA preparation is accomplished quickly and efficiently. The size range fo…
Phosphoramidites for DNA oligonucleotide synthesis, gene synthesis, and more. Features of DNA Phosphoramidites: Amine functions are protected by either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. The recommended procedure for cleavage and deprotection for standard oligonucleotide synthesis is incubation in a concentrated ammonia solution for 8 hours at 55 °C. The purities of manufactured nucleoside phosphora…
Phosphoramidites for RNA oligonucleotides synthesis and more.Features of RNA Phosphoramidites: Amine groups are protected by “either a benzoyl or isobutyryl group {dA(Bz), dC(Bz), dG(Ib)}. Cleavage and deprotection procedures for RNA synthesis is similar to DNA synthesis, with just an additional step to remove the 2'-OH protecting group. The 2'-OH group is protected by a tert-butyldimethylsilyl (TBDMS) group to prevent de…
Amino linker phosphoramidites for the functionalization of the 5'-end during oligo synthesisFeatures• Amino linker products are coupled with standard synthesis protocols, identical to the coupling of DNA monomer phosphoramidites.• 5'-Amino phosphoramidite is used to add a functional amine group at the 5'- or 3'- end of the oligonucleotides. The primary amino group can be used for subsequent conjugation of a variety of repo…
Spacer phosphoramidites for oligonucleotide synthesis. Wide range of lengthsA wide selection of linker lengths available: C3, C4, C6 and C12Features• The spacer phosphoramidite are used to put spacer arms into oligonucleotides.C3, C6 and C12 spacer phosphoramidite contain an aliphatic linker which can be added to oligo requiring longer spacer arms. In general terms, spacer phosphoramidites are used during oligonucleotide s…
Phosphoramidites for nucleic acid labeling, microarray labeling, and moreFeatures• Oligonucleotides modified with fluorescein derivatives can be used for genomic research, molecular diagnostics and molecular biology.• t-butylamine/H2O/MeOH is recommended for cleavage and deprotection steps of TAMRA modified oligos.• dI-phosphoramidite can perform as an universal base as it hybridizes with any of the four bases.Technical Info…
High load CPG prepackaged Universal solid support for DNA SynthesisControlled Pore Glass (CPG) is the gold standard for solid phase oligonucleotide synthesis, with features such as high surface area, tightly controlled pore size and chemical inertness.Our High load CPG for DNA synthesis is available with or without phosphoramidites and can be purchased for standard synthesis sizes or for long-mer synthesis.Features• Utilizes C…
dNTP's from Bioneer are cost effective and hightly pure. Bioneer is one of the few manufacturers that actually produces our own phosphoramidites. Our dNTP's are made at our state-of-the-art manufacturing facilities, and Bioneer controls quality every step of the way in order to provide you with the best value for your research dollar.
