Promega Corp. Products & Reviews

Magne™ Protein G and Magne™ Protein A Beads are magnetic affinity beads with high specificity and high capacity for purification of immunoglobulins from cell culture media, ascites and serum samples.   These paramagnetic beads are composed of iron encapsulated in macroporous cellulose with low nonspecific binding. The magnetic beads use a novel attachment chemistry to immobilize recombinant Protein G or Protein A protein…

The GoTaq® G2 Hot Start Polymerase and Master Mixes simplify your reaction preparation, bringing you the convenience of room-temperature setup and the specificity of a hot start polymerase.   Ready-to-use Master Mix simplifies reaction setup and allows direct-to-gel loading. The optimized formulation provides amplification of a variety of targets, while eliminating non-specific products, resulting in higher yields and gr…

The MagneHis™ Protein Purification System provides a simple, rapid and reliable method for the purification of polyhistidine- or HQ-tagged, expressed proteins.   Paramagnetic precharged nickel particles (MagneHis™ Ni-Particles) are used to isolate polyhistidine- or HQ-tagged protein directly from a crude cell lysate using either a manual (requires use of a magnetic stand) or automated procedure. Using a tube format,…

The TnT® Quick Systems are convenient single-tube, coupled transcription/translation reactions for eukaryotic cell-free protein expression.   These cell-free expression systems combine the RNA Polymerase, nucleotides, salts, amino acids and Recombinant RNasin® Ribonuclease Inhibitor with the reticulocyte lysate solution to form a single TnT® Quick Master Mix. The TnT® Quick Coupled Transcription/Translation System is ava…

Diamond™ Nucleic Acid Dye is a safe, sensitive fluorescent dye that binds to single-stranded DNA, double-stranded DNA and RNA, and can be used to stain and visualize nucleic acids in gels.   Diamond™ Nucleic Acid Dye is compatible with denaturing and native agarose and polyacrylamide gels and can be imaged with any standard imaging system. Significantly less mutagenic than traditional ethidium bromide, as proven by Ames…

The Maxwell® Clinical Sample Concentrator (CSC) automated nucleic acid extraction system is designed for clinical laboratories needing to process multiple samples for critical downstream assays. This easy-to-use, cGMP-compliant system provides consistent, high-quality results with every run, allowing you to manage your workflow and gain efficiency. The purification methods use sample lysis and binding to paramagnetic par…

Designed for use with the Maxwell Clinical Sample Concentrator and the Maxwell CSC blood purification method as an IVD medical device for automated isolation of human genomic DNA from whole blood.   Because the Maxwell® CSC Blood DNA Kit is manufactured to such strict specifications, you can count on consistent, high quality DNA suitable for use in downstream amplification assays. Furthermore the intuitive interface…

The Maxwell® CSC DNA FFPE Kit is used in combination with the Maxwell® CSC Instrument to provide an easy method for efficient, automated purification of genomic DNA (gDNA) from human FFPE breast, lung or colon tissue samples.   The Maxwell® CSC DNA FFPE (formalin fixed, paraffin embedded) kit is intended for use, in combination with the Maxwell® CSC Instrument and the Maxwell® CSC DNA FFPE purification method, as an…

The Glucose-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glucose in biological samples.

The Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamate in biological samples.

The Glutamine/Glutamate-Glo™ Assay is a bioluminescent assay for rapid, selective and sensitive detection of glutamine and glutamate in biological samples.

The Succinate-Glo™ JmjC Demethylase/Hydroxylase Assay is a bioluminescent assay for detecting the activity of JumonjiC (JmjC) histone demethylases and many Fe(II)/α-ketoglutarate-dependent dioxygenases that use α-ketoglutarate as substrate and release succinate as a product.

IdeS Protease is an immunoglobulin-degrading enzyme from Streptococcus pyogenes (IdeS). It is an engineered recombinant protease overexpressed in E. coli that cleaves Immunoglobulin G (IgG) with high specificity at a single site below the hinge region, yielding F(ab’)2 and Fc fragments. The protocol for a standard reaction is to add the IdeS Protease to the IgG sample, add 1 unit of IdeS Protease per 1µg of IgG to be digested…

IdeZ Protease is an immunoglobulin-degrading enzyme from Streptococcus equi subspecies zooepidemicus. It is an engineered recombinant protease overexpressed in E. coli. Like IdeS Protease, IdeZ Protease specifically cleaves IgG molecules below the hinge region to yield F(ab′)2 and Fc fragments. However, IdeZ Protease has significantly improved activity against mouse IgG2a and IgG3 subclasses compared to IdeS Protease.

Automated DNA Extraction from FFPE Tissues

Automated Purification of High-Quality Total RNA with an Enhanced miRNA Enrichment

Automated ccfDNA Purification with a Simple Three-Step Protocol

Automated DNA Extraction from Plant Tissues

Automated RNA Extraction from Plant Tissues

Automated DNA Purification from Whole Blood

Automated DNA Purification from Buffy Coat samples

Safe, Automated DNA Purification from Food and Feed Samples

Automated DNA Extraction from Mammalian Tissue

Automated DNA Purification from Mammalian and Bacterial Cultured Cells

Automated DNA Purification from Stabilized Saliva Tubes