Merck Products & Reviews

EMD Millipore's S1P1 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P1 interaction with its ligands. The cell line exhibits a calcium response with EC50s of 141 μM for Sphingosine 1-phosphate. The membrane preparations exhibit EC50s of 9.5 μM for Sphingosine 1-phospha…

Chemicon's cloned human PK2-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant PK2 expression on the cell surface and contains high levels of the promiscuous G protein Gα15 to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between PK2 and its ligands.

Chemicon's cloned human EP2-expressing cell line is made in the Chem-9 host, which supports high levels of recombinant EP2 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between EP2 and its ligands.

EMD Millipore's EP2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of agonists and antagonists of EP2. The membrane preparations exhibit a Kd of 14.52 nM for [3H]-PGE2. With 7.5 nM [3H]-PGE2, 10µg/well EP2 Membrane Prep typically yields greater than 3-fold signal-to-backgr…

EMD Millipore’s cloned human Ghrelin Receptor-expressing cell line is made in the Chem-1 host, which supports high levels of recombinant Ghrelin Receptor expression on the cell surface and contains high levels of the promiscuous G protein to enhance coupling of the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at Ghrelin Receptor.

EMD Millipore's Ghrelin Receptor membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of ghrelin interactions and its ligands. The membrane preparations exhibit a Kd of 0.32 nM for [125I]-ghrelin. With 0.1 nM [125I]-ghrelin, 5 μg/well Ghrelin Receptor Membrane Pre…

Chemicon's cloned human S1P4-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant S1P4 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P4 and its ligands.

EMD Millipore's cloned human S1P5-expressing cell line is made in the Chem-5 host, which supports high levels of recombinant S1P5 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for antagonists of interactions between S1P5 and its ligands.

EMD Millipore's S1P5 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of S1P5 interactions with its ligands. The membrane preparations exhibit EC50s of 2.4 nM for S1P in a GTPγS binding assay.

EMD Millipore’s cloned human GPR92 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR92 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at GPR92.

EMD Millipore’s cloned human GPR91 -expressing cell line is made in the Chem-1 host, which supports high levels of recombinant GPR91 expression on the cell surface and contains high levels of the promiscuous G protein to couple the receptor to the calcium signaling pathway. Thus, the cell line is an ideal tool for screening for agonists and antagonists at GPR91.

Chemicon's Wild-Type Chem-1 cell line is a control for Chemicon's GPCR-expressing Chem-1 calcium-optimized cell lines. The Chem-1 cell line is adherent to tissue culture-treated surfaces and contains high levels of the promiscuous G protein Galpha15 to couple many classes of GPCRs to the calcium signaling pathway.

ChIP-Seq facilitates genome-wide profiling of the interactions of transcription factors, modified histones, and other chromatin associated proteins. By combining chromatin immunoprecipitation with next generation sequencing, laboratories can generate high resolution data on protein-DNA interactions across the genome. For laboratories interested in gaining a deeper understanding of epigenetic mechanisms of gene regulation, ChIP…

Introducing Direct Detect™, the first infrared (IR)-based biomolecular quantitation system. Just spot your sample, blank, and read! No sample prep, standard curves, messy cuvettes or liquid waste. With inherent amide bond detection, the Direct Detect™ system distinguishes proteins and peptides from interfering sample components, providing more accurate results without the pitfalls of colorimetric assays. Technology: FTIR Spe…

Indispensable for NMR spectroscopy Unlocking the molecular structure of organic molecules is central to understanding how they interact with other molecules. This is especially critical in genomics, proteomics and drug discovery research. The Nuclear Magnetic Resonance (NMR) technique allows scientists to “see” molecular structure and dynamics. MagniSolv™ deuterated solvents are a key tool for the NMR technique. MagniSolv™ deu…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The PureGenome™ High Efficiency Next Generation Sequencing (NGS) Library Preparation Reagents are uniquely optimized as a one-stop solution for NGS Library Preparation. Validated for sequencing compatibility with Illumina® instruments; three sets of reagents are offered each having a unique functionality in the library preparation work flow. The PureGenome NGS Library DNA Modifier, when purchased with the PureGenome NGS Librar…

The quality of sequence obtained from Next Generation sequencing platforms such as Illumina, Inc. requires the accurate determination of input library DNA. Loading too much DNA can lead to overlapping clusters and loss of read counts whereas too little input can lead to poor read lengths and lower sequence output. The PureGenome™ NGS Library Validator Kit measures the absolute amount of adapter-ligated, sequence ready DNA in I…

The PureGenome™ On-Spot Tissue DNA kit allows for convenient isolation of genomic DNA in any targeted microscopic tissue area on a slide. The system combines a powerful area-specific tissue sampling technology with a one-step DNA extraction method and makes targeted tissue genomic DNA isolation easy and simple. The kit efficiently recovers genomic DNA from paraffin archives or fresh tissue sections for PCR analysis. Unlike a c…

The PureGenome™ Tissue DNA Extraction Kit is a simple procedure for the rapid isolation of total DNA (e.g. genomic, mitochondrial, parasitic, microbial, viral) from a variety of soft and solid tissues, cells and a range of biological liquids. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is also compatible with buffy coat, bone marrow, cells from culture, whole blood (fres…

A sterile, closed, disposable system for sampling fluids from sterile and aseptic processes The unique NovaSeptum sampling system is ideal for general fluid and cell culture sampling from aseptic and sterile processes. Both systems can be configured for high purity or autoclavable sampling applications and as single or multiple sampling units. A wide range of holders and connectors are also available. •  Closed sampling elimin…

Containers Make Sense EMD Millipore ReCycler™ Containers guarantee solvent purity and meet the needs of the scientific community by eliminating the need to dispose of large volumes of glass bottles, styrofoam packaging inserts and corrugated cartons. Today, EMD Millipore ReCycler™ containers are available in sizes from 18.9L (5 gallon) to 1250L for the many diverse scientific applications from laboratory to small-scale manufac…

Pure recombinant high-fidelity DNA polymerase from Thermococcus kodakaraensis KOD1KOD HiFi DNA Polymerase* is a recombinant form of Thermococcus kodakaraensis KOD1 DNA polymerase. KOD HiFi is a high fidelity thermostable polymerase that amplifies target DNA up to 6 kbp in length with superior accuracy and yield. The enzyme’s 3′→5′ exonuclease-dependent proofreading activity results in a lower PCR mutation frequency than any ot…

High-performance enzyme blend for long and accurate PCRKOD XL DNA Polymerase* is an optimized blend of KOD HiFi DNA Polymerase and a mutant form of KOD HiFi that is deficient in 3′→5′ exonuclease activity. This enzyme mixture is designed for reliable amplification of long, complex targets with robust yield and high accuracy. It can also be used for incorporation of dITP, dUTP and derivatized dNTPs in PCR amplicons. KOD XL DNA…