The His•Tag® Monoclonal Antibody is a mouse monoclonal antibody (IgG1) directed against the His•Tag sequence encoded by many of Novagen's expression vectors as well as many other commercially available vectors. The antibody recognizes five consecutive histidine residues regardless of the surrounding amino acid context. The high affinity (Kd = 5 × 10 to 8–1 × 10–9 M) enables sensitive, specific detection of His•Tag fusion prote…
Benzonase Nuclease - Effective viscosity reduction and removal of nucleic acids from protein solutions Benzonase Nuclease is a genetically engineered endonuclease from Serratia marcescens. It degrades all forms of DNA and RNA (single stranded, double stranded, linear and circular) while having no proteolytic activity. Benzonase Nuclease is effective over a wide range of conditions and possesses an exceptionally high specif…
Purification of His•Tag fusion proteins by metal chelation chromatographyThe His•Bind® family of products offers a wide selection of supports designed for rapid one-step purification of proteins containing the His•Tag® sequence by immobilized metal affinity chromatography (IMAC). The His•Tag sequence (6, 8 or 10 consecutive histidine residues) binds to divalent cations (Ni2+) immobilized on NTA*- and IDA-based His•Bind resins.…
The most common method for transformation of plasmids into E. coli is to use chemically competent cells. Although competent cells can be prepared in the laboratory, greater efficiency, reproducibility and convenience are achieved using prepared competent cells. Novagen offers the widest selection of competent cells available for protein expression. Our strains offer a variety of options that enable you to maximize the yield an…
Pseudoproline dipeptides are undoubtedly the most powerful tools described to date for enhancing synthetic efficiency in Fmoc SPPS. Novabiochem® now offers 28 pseudoproline dipeptides - representing the widest selection on the market today The routine use of pseudoproline dipeptides has been found to: Avoid costly and unnecessary repeat syntheses of failed sequences Increase purity of crude products Simplify HPLC puri…
Calbiochem® supplies a wide variety of products for the study of apoptosis, includingActivators and Inhibitors Antibodies and Blocking Peptides Assay Kits Caspase-related products Mitochondrial Permeability-Transition Reagents and Related Products Poly(ADP-Ribose) Glycohydrolase (PARG) Inhibitors Poly(ADP-Ribose) Polymerase (PARP) Inhibitors Telomerase Products View the online interactive pathways to easily locate the product…
The pET System is the most powerful system yet developed for the cloning and expression of recombinant proteins in E. coli. Based on the T7 promoter-driven system, Novagen’s pET System has been used to express thousands of different proteins. pET Vectors are supplied as purified plasmid DNA (10 µg) plus an Induction Control strain (supplied as a glycerol stock). Complete sequences and detailed vector maps are available.
The D-Tube™ Dialyzers can be used for dialysis and electroelution of proteins or nucleic acids from polyacrylamide or agarose gels. The disposable, single-use tubes require no syringes, microcentrifuges, or laborious steps to manipulate small samples volumes. The sample is added and removed using a standard laboratory pipette. Available with molecular weight cut-offs from 3.5 to 14 kDa, the D-Tube Dialyzers are designed in thr…
Quick and Easy Antibody Quantitation:With its simplicity and ease of use, Guava’s new RapidQuantTM IgG Kits have raised the bar for IgG titering assays. Designed to be time-saving, labor-saving and money-saving, Guava’s RapidQuant kits eliminate the most tedious, laborious portions of antibody titering methods with Guava’s signature ease of use. • Robust, rapid and highly reproducible • Relevant quantitative range • Quantifies…
Eshmuno® CPX media - High aggregate removal efficiency combined with high dynamic binding capacity Eshmuno® CPX media is a strong cation exchanger built on the proven Eshmuno® resin technology. Eshmuno® CPX media combines high aggregate removal efficiency in downstream purification linked to an outstanding high dynamic binding capacity, utilizing the 50 μm Eshmuno® base bead technology and EMD Millipore’s proprietary tentacle…
The FlowCellect™ Human NK Cell Characterization Kit includes three directly conjugated antibodies, Anti-CD3-APC, Anti-CD56-PE, and Anti-CD16 FITC. This three color kit is designed to characterize NK within mixed cell populations. The antibodies provided have been carefully titrated to ensure the ability to accurately measure the expression of all markers simultaneously. Sufficient reagents are provided to perform 25 three-colo…
SmartFlare Probes work by recognizing specific native RNA sequences within living cells, therefore there are many possible sequences that you could design to recognize your target of interest. Instead of lysed cells, switch to live cells. And while you’re at it, eliminate sample preparation and transfection steps all together. Live cell RNA detection is now possible, in a single incubation step using inert nanoparticle techno…
Introducing an all-in-one centrifugal device for the protein purification workflow.Traditional protein purification is a long process with many steps and multiple devices, often resulting in protein degradation and loss. Avoid the risks associated with sample transfer and reduce hands-on time when you bind, wash, elute and/or concentrate your protein in the all-in-one Amicon® Pro purification system. No matter what your workfl…
The SIRTainty™ Class III HDAC Assay utilizes a novel patent pending technology for the sensitive detection of all known sirtuin family members. This easy to perform assay employs nicotinamidase to measure nicotinamide generated upon cleavage of NAD+ during sirtuin mediated deacetylation of a substrate providing a direct assessment of the activity of class III HDAC enzymes. Unlike conventional assays that are dependent upon a s…
Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Panel 1 (Cat. No. 48-617MAG) is a premixed panel to be used for the simultaneous quantification of the following 4 analytes in cell lysates: c-Myc, Nanog, Oct 3/4, and Sox2. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has sufficient reagents for one 96-well plate assay. Backgro…
Product Overview: The MILLIPLEX® Human Stem Cell Pluripotency Magnetic Bead Kit 2 (Cat. No. 48-620MAG) is a premixed kit to be used for the simultaneous quantification of the following 7 analytes in cell lysates: E-Cadherin, EpCam, KLF4, LIN-28, and the glycostructures TRA-1-60, TRA-1-81 and SSEA1. The detection assay is a rapid, convenient alternative to Western Blotting and immunoprecipitation procedures. Each kit has suffic…
Millipore’s FlowCellect™ DNA Damage (Histone H2A.X) and Cell Cycle Analysis Kit is designed to identify the linkage between DNA damage in relation to cell cycle checkpoints in flow cytometry applications. The kit includes a directly conjugated Anti-phospho-Histone H2A.X (Ser139) Alexa Fluor® 488 antibody plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to identify where DNA damage ta…
Millipore’s FlowCellect™ DNA Damage (ATM) and Cell Cycle Analysis Kit is designed to identify the linkage between DNA damage in relation to cell cycle checkpoints in flow cytometry applications. The kit includes a directly conjugated Anti-phospho-ATM (Ser1981) Alexa Fluor® 488 conjugate plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to identify where DNA damage takes place relativ…
Millipore’s FlowCellect™ Bivariate Cell Cycle Kit for DNA Replication Analysis is designed to investigate cells undergoing replication in S-phase of the cell cycle with high accuracy and confidence in flow cytometry applications. The kit includes a directly conjugated Anti-BrdU Alexa Fluor® 488 conjugate plus a DNA dye (Propidium Iodide) which allows the researcher to perform bivariate analysis to distinguish cells in two dim…
Millipore’s FlowCellect™ Bivariate Cell Cycle Kit for G2/M Analysis is designed to allow the researcher to easily identify, discriminate, and quantitate cells between the G2 and M-phases of the cell cycle. The kit can be used to perform bivariate analysis to measure cell cycle distributions by discrimination of cell cycle phases without the need of sophisticated software modules or algorithms. Having the ability to distingui…
Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit is designed to enable a researcher a quick and easy way to detect the phosphorylation state of ATM, SMC1 and Histone H2A.X by flow cytometry. Millipore’s FlowCellect™ Multi-Color DNA Damage Response Kit was developed and tested using the DNA damaging reagent, Etoposide, in HeLa cells as a model system but the kit can be used with other human cell lines to determine…
The Magna ChIP HiSens kit provides a complete set of validated, quality controlled reagents, and a detailed protocol to enable ChIP from a wide range of input amounts of chromatin obtained from either cells or tissues. Our specialized blend of protein A/G blend of magnetic beads is specifically produced for chromatin immunoprecipiptation and enables the use of a broader range of antibodies than protein A or G alone eliminating…
The PureEpi Chromatin Preparation and Optimization kit provides a complete set of reagents allowing the preparation of multiple chromatin samples from starting materials such as cultured cells or tissues. A detailed protocol is provided that includes examples of the many variables involved in making chromatin on multiple instruments and how they may affect ChIP results. Features & Benefits • Complete set of materials for up to…
Eshmuno® A is a rigid, high capacity Protein A affinity chromatography resin for the purification of Fc-containing proteins, including but not limited to monoclonal antibodies. Eshmuno® A resin can be cleaned and sanitized under acid and alkaline conditions while maintaining high dynamic binding capacity at high flow rates.The Eshmuno® A resin contains a EMD Millipore proprietary ligand. This ligand is recombinantely expresse…
