Agilent Technologies UHPLC and HPLC Products & Reviews

Increase resolution where you need it with the 1290 Infinity III 2D-LC System, which offers comprehensive two-dimensional liquid chromatography (2D-LC), (multiple) heart-cutting 2D-LC analysis, and high-resolution sampling. This system allows you to use 2D-HPLC for making buffer separations compatible to MS detection, while easy-to-use software enables fast method setup and refined data analysis.

InfinityLab Quick Connect fittings are the only truly "finger-tight," reusable fitting for UHPLC, stable to 1300 bar. There are no tools needed… just use your fingers to close the lever for a perfect connection every time.

In order to more easily isolate and identify proteins in human serum, the Agilent Human 14 Multiple Affinity Removal Column is designed to chromatographically remove fourteen interfering high-abundant proteins from human plasma samples.

The 1260 Infinity II Prime LC is a versatile HPLC offering the highest functionality and operational convenience for analytical HPLC and entry-level quaternary UHPLC at pressures up to 800 bar and for flow rates up to 5 mL/min. This versatile HPLC system is a perfect front end for your MS system. 

Agilent AdvanceBio SEC Protein Standards are uniquely designed for AdvanceBio SEC columns. The standards are manufactured from a select group of proteins and peptides optimized to deliver accurate, precise quantitation for mAb aggregation and protein analysis. 

Agilent Human Milk Oligosaccharide (formerly ProZyme) standards are highly purified standards for qualitative methods.

Agilent Poroshell columns are ideal for fast separations of proteins and peptides because the unique particle allows for fast flow rates to be used while maintaining sharp, efficient peaks. 

The 1260 Infinity II Preparative Binary Pump is an affordable high-pressure gradient pump for LC purification. The pump offers a dynamic flow rate range from 1 to 50 mL/min at up to 420 bar, allowing workflows on columns from 9.4 to 30 mm inner diameter.

The 1260 Infinity III RID is an HPLC refractive index detector (RID) detector that provides excellent performance for entry-level UHPLC and HPLC differential refractometer (DRI) experiments.

The 1260 Infinity III Diode Array Detector HS, with a fixed slit design, is based on the Agilent Max-Light Cartridge Cell with optofluidic waveguides, which improve light transmission to near 100% efficiency without sacrificing resolution caused by cell dispersion effects.

The 1260 Infinity III Fluorescence Detector offers time-programmable excitation and emission wavelength switching. The proven optical and electronic design provides the highest sensitivity for the analysis of trace-level components. This detector can be used with any Agilent InfinityLab LC Series system or other LC instrument.

The 1260 Infinity III Fluorescence Detector Spectra is a sensitive HPLC fluorescence detector that simultaneously provides quantitative data and fluorescence spectra from a single run, enabling rapid method optimization for HPLC FLD Detector Spectra experiments.

The 1290 Infinity II Bio LC System is a UHPLC, binary or quaternary ,- consisting of biocompatible material for use in biopharma (e.g., critical quality attributes) and other applications utilizing high salt and extreme pH conditions. Biocompatibility ensures the integrity of your biomolecules and robustness of the system.

An ideal solution for trace level PFAS analysis with LC/MS/MS

The 1290 Infinity II Bio Flexible Pump is a UHPLC pump consisting of biocompatible material, designed for use in biopharma and other applications utilizing high-salt and extreme-pH conditions. It offers gradient formation from up to four solvents at pressures up to 1300 bar and flow rates up to 5 mL/min.

InfinityLab solvent glass reservoirs are designed to provide high levels of performance for your LC applications.

The new InfinityLab lamps contain RFID technology for more convenience and ease of use. Agilent lamps are manufactured in an ISO 9001 certified environment and are fully traceable throughout every step of the production process. Each lamp is tested to ensure it meets Agilent performance specifications.

Agilent ZORBAX Eclipse XDB columns – C18, C8, Phenyl and CN – provide four bonded phase choices for method development optimization. These columns provide good peak shape over a wide pH range (2–9) for additional method development flexibility with one family of columns. Eclipse XDB columns can be used for method development at low pH (2–3) and the same column can be used for method development in the mid pH (6–8) region.

Poroshell 120 columns provide rugged, high resolution separations within the pressure range of any mainstream LC system, making the benefits of sub-2 µm performance available with all existing LCs.Poroshell columns can achieve high resolution and high speed separations on current instruments, and higher resolution and speeds on new high-pressure LC and LC/MS systems. Stable to 600 bar, Poroshell 120 are great for fast UHPLC an…

OpenLab CDS EZChrom provides superior control of Agilent LC and GC instruments, plus the most complete control of other vendors’ instruments.

Polar-modified column; available in 3.0, 4.0, 4.6, 10, 21.2 and 50 mm id;  30 - 250 mm length. From the collapse-resistant pore structure of the base silica, to the "wettability" engineered into the bonded phases, Polaris columns have been designed for high aqueous conditions.  The combination of high-phase density bonding, ultra pure silica and silanol shielding leads to excellent peak shape among polar-modified columns.

ZORBAX 80Å StableBond columns are made using bulky, patented, unique silanes that sterically protect the siloxane bond. Acid labile endcapping reagents are not used. The result is vastly improved column life and extraordinary chemical and temperature stability in the pH 1–6 range for a wide variety of phases (SB-Aq, SB-C3, SB-CN, SB-Phenyl, SB-C8, and SB-C18). Excellent peak shape is expected for many basic compounds because…

Bonus-RP columns provide unique selectivity and applicability for mid pHs with an embedded amide linkage in the C14-alkyl chain. At pH 7 Bonus-RP provides excellent peak shape for even the most challenging basic compounds.

The 1290 Infinity II Refractive Index Detector (RID) is a UHPLC RID detector that provides excellent sensitivity, resolution, and sample throughput for UHPLC DRI experiments. The 1290 Infinity II RID is a equipped with an ultralow dispersion microflow cell and a data rate of 148 Hz, which significantly reduces run times for higher sample throughput and improved resolution and sensitivity.

The 1260 Infinity II Degasser is suitable for analytical applications with flow rates up to 10 mL/min and up to four solvents. It is highly recommended for GPC/SEC applications using refractive index detection. It reduces baseline noise and quenching effects due to high degassing capacity.