Zyppy™ Plasmid Miniprep Kit by Zymo Research

Manufacturer Zymo Research  |   Model: D4019
4.7
/
5.0
  |  81 reviews
The Zyppy™ Plasmid Miniprep Kit is the fastest and simplest method available to efficiently separate plasmid DNA from E. coli.


Zyppy™ Plasmid Miniprep Kit by Zymo Research product image
Zyppy Plasmid Miniprep Kit

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Average Rating: 4.7
81 Scientists have reviewed this product

5 out of 5
Ease of use
4 out of 5
After sales service
5 out of 5
Value for money


  • Status:

    Reviewer
  • Member since: 2019

  • Organization: Touro University Nevada



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast, reliable plasmid isolation mini-prep kit.
Rating: 5.0

  • Application Area: Isolate plasmids for transfection

"I started using Zyppy Plasmid kit from a free sample and I am hooked. It is so fast and easy to use! I have used the ZymoPure miniprep kit as well. Both work well, though Zyppy is faster. I recommend this kit because of its speed. The quality of the DNA plasmids that I recover is also good."

Review date: 13 Dec 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Institute of Molecular Genetics



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use, fast, reproducible, and good quality
Rating: 5.0

  • Application Area:Plasmid purification for in vitro studies and cloning

"Have used this product for years. Very easy to use, coloured buffers help to visualize neutralisation, high yield, good reproducibility, plasmids can be used for in vitro studies and for cloning as well, and it works so fast (this parameter is very important for me)! Almost all steps require just 15 seconds of centrifugation – usually, I spin down 4ml of culture and add 500ml of mQ to get 600ml final volume to start plasmid purification."

Review date: 11 Dec 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Csu



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Very simple to get high concentration
Rating: 5.0

  • Application Area:Molecular cloning

"The product offers simple direction and ease of use, so we are able to give it to the entire lab to use without a problem"

Review date: 11 Dec 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: UT Health San Antonio



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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If you make your product better we will buy it again.
Rating: 4.3

  • Application Area:E. coli bacteria for cloning

"The DNA concentration was not high, your price is good, but we can't buy large kits because the shelf life of the kit is very short. They always expire so quickly we can't keep them longer."

Review date: 27 Nov 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Rochester



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use, reliable and fast.
Rating: 5.0

  • Application Area:Plasmid isolation from E. Coli

"This product provides the fastest way to isolate DNA plasmids from E. Coli. It is reliable, easy to use and the purified plasmids are endotoxin-free and ready for transfection!"

Review date: 27 Nov 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Chicago Medical School



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great results without any issues.
Rating: 5.0

  • Application Area:Miniprep for DNA injection into C. elegans

"It is easy to use, and its quality is beyond expectation! We use plasmid DNA for the injection into C. elegans to generate transgenic lines without any further purification."

Review date: 27 Nov 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Georgia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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More than enough DNA.
Rating: 5.0

  • Application Area:Metabolic engineering

"The kit is easy to use and provides plenty of plasmid DNA to use in down-stream work. It is cheaper than it’s competitors in the market."

Review date: 27 Nov 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Chicago



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Reliable and efficient DNA prep.
Rating: 4.7

  • Application Area:Preparation of dNA to screen or sequence

"The Zyppy MiniPrep kit is an efficient spin based protocol for the production of plasmid DNA for most routine DNA applications, we have excellent results in both preparative approaches - subcloning, PCR as well as screening. Our favorite kit to date."

Review date: 27 Nov 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Madurai Kamraj University, Dept. Molecular Biology, School of Biological Sciences



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    2 out of 5
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I have a chance to use kits for my experiments that give better results.
Rating: 3.0

  • Application Area:Isolate genomic plasmid DNA from bacterial samples

"Very good product for isolation of genomic, plasmid DNA from bacteria. I achieved high quality yield and also reproducible results. I used the above mentioned product only in overseas labs (Israel). Easy to use and handle. Minimum number of solutions for better results."

Review date: 11 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: CSIR



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Value for money for everyday use.
Rating: 4.3

  • Application Area:Plasmid DNA isolation from E. coli

"Very easy to use. Plasmid quality is consistent, although not the best yield and concentration. Other, more expensive kits give higher quality DNA, but for everyday screening, this kit is value for money."

Review date: 07 Jun 2019 | Zyppy™ Plasmid Miniprep Kit


  • Status:

    Reviewer

  • Member since: 2019

  • Organization: INSERM



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    4 out of 5
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Good product, consistent results.
Rating: 4.7

  • Application Area:Bactera transformation

"So far, this kit has been perfect. I have been able to easily make my minipreps, consistently."

Review date: 06 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Cardiff University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Quickest and highest recovery - no brainer really.
Rating: 4.3

  • Application Area:Protein expression

"I carried out a comparison against 2 other major brands of plasmid mini prep kit, purifying plasmid from 1ml of pelleted bacteria. The Zyppy kit was the quickest and cheapest per sample (at the time, I haven't compared prices since) of the three. In terms of yield, it also had the highest recovery of our plasmid, which is much larger than average. The kit is simple to use - my only warning being to make sure you add ethanol to the wash buffer when you receive the kit to prevent students using the concentrated solution by mistake. I have no experience of the after sales care."

Review date: 06 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Providence College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The technical support is unbeatable!
Rating: 5.0

  • Application Area:Prepare plasmids for transformation.

"The plasmid miniprep kit is very easy to use. On occasions when I have had difficulties and my students obtained poor results, I called tech support and spoke to Michelle and she was able to troubleshoot the problem for us. It's really great to know that whenever I have a problem, I can call tech support and obtain the help that I need."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Promab Biotechnologies Inc.



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great miniprep kit, my go-to.
Rating: 5.0

  • Application Area:DNA purification

"I have used many different kits to do minipreps and this one is the easiest and quickest I have used so far, and it is also cheap."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: STANFORD UNIVERSITY



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast minipreps with high yield.
Rating: 5.0

  • Application Area:DNA isolation

"Much better than Qiagen columns, with higher yield and a shorter protocol!"

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: HHMI - Stanford



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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We use this product a lot. Zymo has replaced Qiagen in our lab.
Rating: 5.0

  • Application Area:Molecular biology and glycomics

"Zymo products have helped us stay on budget, while providing exceptional products."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of South Carolina Aiken



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Problem free.
Rating: 5.0

  • Application Area:Molecular cloning

"This kit is easy to use and reliable. I run a primarily undergraduate laboratory and they rarely have any problems. We have recommended this kit to others at our university and they also have been pleased."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: ATUM



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Easy and quick kit for plasmid DNA.
Rating: 4.3

  • Application Area:Purify plasmid DNA from E. Coli for transfection

"The product is easy to use and provides very low level endotoxin plasmid DNA. Its also fast - I can complete 20 samples in under 20 minutes!"

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: CELLSCRIPT, LLC



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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It just works.
Rating: 4.3

  • Application Area:Plasmid stock for clone screening.

"Haven't needed customer support because the kit works, and works well. Very straight forward and simple protocol. Plasmid is ready in a timely fashion. Have not had any problems with the kit ever."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Ruhr Universität



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast and easy at a good price.
Rating: 5.0

  • Application Area:Plasmid purification

"Fast and easy to use with colored buffers to show when the lysis and neutralisation steps are complete. High yield and the price is very good."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: hhu



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great.
Rating: 4.3

  • Application Area:Cell culture

"Good value, we love this product. Reproducible results."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Georgia



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Good result.
Rating: 4.7

  • Application Area:Cloning for In situ hybridization

"Even though yield is little bit low, quality of plasmid prep is good."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Stellenbosch University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Great.
Rating: 4.3

  • Application Area:Yeast plasmid extraction

"High quality DNA concentration. Higher yields when buffer or water is warm. Easy to use."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Emory University Department of Human Genetics



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Excellent product, would be great to see it in a 96-well format.
Rating: 4.3

  • Application Area:Isolation of plasmids to screen clones

"This product worked great everytime with clear directions. The only reason for a 3 star after sales care score was because I didn't need any after sales instruction. I will continue to use this kit for restriction digest screening of clones."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: University of Stuttgart - Institute for Technical Biochemistry



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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I cannot think of any reasons why I should buy any other kit.
Rating: 5.0

  • Application Area:Enzyme engineering

"I appreciate the easy use of the kit and the purity of plasmid DNA after preping out of E. coli cells. Even students can use the kit without anything else than the provided manual and get very good results. I think it would be even a bit better, if there were small symbols or something like that on the coloumns to identify them in the centrifuge without having to write on the very small labelling areas. The numbers given there are very small and not so easy to read (actually I'm not even sure, if they are meant for identification or if they are just remains from the production process)."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2019

  • Organization: Molekulare Physikalische Chemie



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Perfect.
Rating: 4.3

  • Application Area:Analysis of CpG methylation in urothelial cancer genes

"I have performed hundreds of mini preps. I can say the following: very easy and intuitive handling, reproducible results in terms of quality and concentration acceptable price and benefit."

Review date: 05 Jun 2019 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Augustana College



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Easy to use and effective
Rating: 4.7

  • Application Area:Gene cloning applications

"The product was very easy for my undergraduate students to use and understand. Consistently high-quality samples were obtained."

Review date: 27 Apr 2018 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: academia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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The only mini I choose to purchase!
Rating: 5.0

  • Application Area:Plasmid propagation

"Easy application, fool-proof for beginners. Great cost and time!"

Review date: 28 Apr 2017 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Cleveland State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Always order this from here! Great results.
Rating: 5.0

  • Application Area:Isolate plasmids

"It is very easy and fast to use. It gives great results."

Review date: 21 Nov 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: ANSES



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast and easy-to-use
Rating: 5.0

  • Application Area:Plasmid purification

"Very fast; no isopropanol precipitation step. Easy to use; few steps and colored buffers to check if each step was run successfully. Great DNA yield and quality."

Review date: 24 Aug 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: California Lutheran University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast kit, reproducible results
Rating: 5.0

  • Application Area:Prepare plasmids for transformation/protein over-expression in E. coli

"We grow a larger overnight culture, then pellet the cells and resuspend in 600 uL to prepare as directed. This does an excellent job of creating high concentration of pure plasmid that reliably works for us in transformation as well as mutagenesis."

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCSD



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Convenient and fast, would like lids though.
Rating: 5.0

  • Application Area:Cloning for protein identification and expression

"The Zyppy Plasmid Miniprep kit was the first kit that I used that has a color indicator added to show when lysis has been fully neutralized. Because of this feature, the quality and yield of the DNA is better than what I experienced in earlier kits from at least 4 other brands. Some steps have been made more convenient, e.g. there is no need to empty the collector tube between the 2 wash steps. These little things make the entire process just that little faster. I don't like that you have to centrifuge the spin columns without a lid. A tip for resuspending the cells: add only 300 uL first, rash the tubes over the top of a tube rack and then add another 300 uL and the 100 uL lysis buffer. The method doesn't work as well with the 600 uL at once."

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCSD



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great isolation plasmid kit
Rating: 4.7

  • Application Area:Cloning of different gene fragment, PCR sub cloning,

"The kit can provide fastest way to get plasmid DNA. Also it is easy to use with highly reproducible results. The instruction is quite simple and user-friendly. The price of the kit is very competitive with other similar kits."

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: UCSF



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Quick n Easy
Rating: 4.3

  • Application Area:Molecular Biology

"The Zymo Miniprep kit is extremely easy to use, takes less time than most other kits and gives great results!"

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Tulane National Primate Research Center



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great yield with minimum starting material
Rating: 5.0

  • Application Area:DNA Isolation

"I would give this product 5-star rating. This kit requires less starting material, yield is great in less time and the quality is fantastic. I never get spark when I perform electroporation. Wonderful products."

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: School of Medicine, University of Maryland



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Streamlined pellet free miniprep.
Rating: 4.0

  • Application Area:Plasmid purification for cloning and transfection purposes

"Zyppy Plasmid Miniprep Kit has removed the cumbersome step of pelleting the bacterial culture before the lysis step. Surprisingly enough the yield is good enough for all the downstream applications that one might need the purified plasmid for. It has made life easier!"

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Advanced Reviewer

  • Member since: 2016

  • Organization: University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great kit and save money and time.
Rating: 5.0

  • Application Area:iPSC cell and neural cell differentiation

"That kit is good, high yield and purity is good, I am satisfied with it."

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Tytgat Institute AMC



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Allows rapid and very efficient plasmid screening.
Rating: 4.7

  • Application Area:Screening clones for correct plasmid

"The Miniprep kit is very easy to use. The isolated plasmid is very pure and can be used for all subsequent steps."

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Michigan Medical Center



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Easy to use, quick and reliable
Rating: 4.3

  • Application Area:DNA purification

"Easy to use, quick and reliable plasmid mini-prep kits. And in fact we use Zymo Midi and maxi prep kits as well as the pcr clean kits and RNA clean kits"

Review date: 29 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Providence College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Undergrad-proof with excellent and quality yields.
Rating: 5.0

  • Application Area:Isolate plasmids from bacteria.

"My undergraduate students use this miniprep kit to isolate all the plasmids we routinely use in the lab. It is undergrad-proof, with excellent and quality yields!"

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Calgary



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    3 out of 5
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Fine product, price a little too high.
Rating: 4.0

  • Application Area:DNA purification (plasmids)

"Excellent product that produces high quality plasmid preps and is easy to use. However, in Canada, it is not competitive with several other products (BioBasic, FroggaBio) based on price. Cheaper than Qiagen last time I checked, and about the same as NEB Monarch."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NCSU/UNC Joint Biomedical Department



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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A consistent mini prep kit for undergraduates and graduates alike.
Rating: 5.0

  • Application Area:Plasmid Isolations

"Ease of Use- I rated this as mainly on my own wet lab experience using Zymo and Qiagen kits and also teaching to undergraduates. Yields from DH5 alpha E coli so far have been in acceptable ranges (100-300 ng/ul , 260/280 ratios near 1.7,1.8). Some optimizations and changes can be made like using water instead of the final elution buffer (for convenience). The kit is easy to show undergraduates and they can perform the mini preps within a week reliably. The color changes are consistent and comparable to other kits in ease. After-Sales Care- I rated this mainly on shipping and ordering. We receive shipments intact and on time when we order from the Zymo site. No shipping issues so far. Value for Money- I rate this mainly on my ordering experience in the lab. The price is comparable to other kits."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Weill Cornell Medical College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Hands down the best miniprep I've ever used... and the yield is anything but mini.
Rating: 5.0

  • Application Area:Plasmid purification for sequencing and transfection

"This prep is super fast, and gives a seriously large amount of transfection-quality DNA from a small culture volume. If I put all the bugs from a 3ml overnight through this kit I've gotten up to 35µg of DNA in a 10 minute prep. I basically don't need any other prep for routine transfections."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: MIT



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great.
Rating: 4.3

  • Application Area:DNA isolation

"Fastest product in the market. Quality of the DNA is superior."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Faculty of Agriculture, University of Zagreb



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Really happy about how efficient is the extraction and the purity of the product.
Rating: 5.0

  • Application Area:Extraction of plasmid DNA

"I got the "Zyppy™ Pasmid Miniprep Kit" to try it out. I must say that Zymo did not fail me, once again! The plasmids that I have extracted were pure and in record time. I know that the price for the kit is very competitive and even more economically friendly. The buffers have normal life span and are kept at room temperature and some are kept at 4 degrees. Neutralization buffer already has RNase inside so you don’t even have to make that post treatment afterwards."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Old Dominion University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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I won't even consider buying another plasmid kit.
Rating: 5.0

  • Application Area:Plasmid minipreps for PCR and cloning.

"This kit is fast, simple and spot-on. We have not had a dud yet that wasn't due to UE (undergraduate error). As a matter of fact, we purchase the kit in the large (400 column) size on a regular basis. The buffers that come with the kit are ample and have a long shelf life. Zymo also helpfully sells the columns separately, so you can get the most value for your money and use all the elements so that nothing goes to waste. On the other hand, you can also buy the buffers separately, so if something mysteriously happens to one of the buffers (as in ending up all over the floor), you can rest easy that you can purchase just the spilled item and not have to buy an entire kit for one component. I've had great results with Zymo technical support and they don't try to upsell you on something that doesn't fit your needs. Oh, yes, and the Miniprep Plasmid Kit is inexpensive (really inexpensive!)."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Univ of California San Francisco



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Great value, can't live without this kit.
Rating: 4.7

  • Application Area:Purify plasmid DNA from bacterial culture

"Fast, without the hassle of taking multiple Eppendorf tube in and out of microcentrifuge for spinning. The quality of final plasmid DNA is as good as Qiagen QIAquick Plasmid Miniprep Kit."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Institute of Molecular Biology



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fastest, easiest, and most cost effective.
Rating: 5.0

  • Application Area:Molecular cloning of DNA constructs.

"These are the fastest, easiest, and most cost effective plasmid prep kits we've tested. Importantly they require the least amount of peripheral equipment, e.g. centrifuges/tubes, vacuum pumps, etc."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Florida State University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    3 out of 5
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Great product.
Rating: 4.3

  • Application Area:Study phosphorylation cell culture

"Easy to use, everything is clear. Always get great DNA yield. Fast protocol leading to fast results. Consistent results. "

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Tel Aviv University



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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It will be very hard to convince us to switch to other products.
Rating: 4.3

  • Application Area:DNA purification for downstream protein expression

"This is one of the best products in the category! Best value for money, easy to use, reproducible yield and high quality DNA. Our sequencing issues were solved by using these kits."

Review date: 28 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Houston



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Ideal results! Little time involved with high quality.
Rating: 5.0

  • Application Area:Plasmid isolation

"The Zyppy™ Plasmid Miniprep Kit was easy to use and gave high clean concentration of the vector."

Review date: 27 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Iowa State University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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It is a high quality & excellent product with a cheaper price.
Rating: 4.7

  • Application Area:Preparation of plasmid DNA from bacterial culture

"The Zyppy™ Plasmid Miniprep Kit is an amazing one, which offers not only high quality plasmid DNA but also good quantity. Most of the time it gave good quality and quantity of DNA and it works better than the Qiagen Miniprep Kit. So the downstream DNA sequencing of the plasmid DNA is very good. Also with this kit the plasmid DNA isolation is very easy with the columns and most importantly I can easily monitor the lysis with the blue dye. Very very important for me is the price is way cheaper than Qiagen Miniprep Kit for the same or better quality plasmid DNA isolation. Our lab saves so much amount of money every month by using this kit. Also the customer care is good in terms of technical support."

Review date: 27 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: university of california, san francisco



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use, affordable.
Rating: 5.0

  • Application Area:Extract plasmid from bacterial cultures

"The product is easy to use and the low elution volume columns are great if you need high concentration plasmid from low-copy number plasmids."

Review date: 27 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: USC



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Bang for the buck.
Rating: 4.0

  • Application Area:Immortalized cell lines & tissue.

"Our yield is decent as well and the quality of the yield is good for our downstream applications. The pricing is great."

Review date: 26 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Public Health Agency of Canada



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Excellent product.
Rating: 4.3

  • Application Area:Plasmid isolation.

"It’s super easy to use, faster than most products on the market and better recovery of plasmid from prep."

Review date: 26 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Tennessee



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Awesome
Rating: 5.0

  • Application Area:Molecular biology

"The best in the market with respect to quality and price."

Review date: 25 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: The Scripps Research Institute



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Affordable and easy to use
Rating: 5.0

  • Application Area:Molecular Biology

"Pretty easy to use with a color based reagents. At the same amount of final material compared with other brands however the rapidness and easiness of the technique makes Zymo products a very good choice."

Review date: 21 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of California, SF



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fast purified plasmids
Rating: 5.0

  • Application Area:Molecular biology

"I use Zyppy Plasmid Miniprep kit to replace vendor Q miniprep. Zyppy Miniprep is fast and easy."

Review date: 21 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Massachusetts Medical School



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Zyppy miniprep kit gives reproducible results and it's very easy to use!
Rating: 5.0

  • Application Area:Extract plasmid DNA

"I can always expect high quality and reproducible results from using Zyppy miniprep kit! Best part is that endo wash step allows in vivo use in downstream experiments."

Review date: 21 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Pennsylvania



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Easy to use, fast results, good price and friendly customer service.
Rating: 5.0

  • Application Area:Cloning and subcloning for extracting Endotoxin free DNA.

"I have been using this kit since it became available - it is the most easy to use kit I have ever used. It delivers endotoxin free DNA for me to sequence. It only takes about a few minutes to get what I need. The price is very good as well. I have recommended it to many of my friends in biomedical research field."

Review date: 18 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NCI



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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This kit is fit for plasmid sequencing!
Rating: 4.3

  • Application Area:Methylation examination for ES cell

"Plasmid extraction is easy and fast! The cost for every sample is low!"

Review date: 14 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: krannert Institution of Cardiology, Indiana University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great product, we've been using it for years and this product has never let us down.
Rating: 4.3

  • Application Area:Plasmid DNA extraction and purification

"This kit is easy to use and produces good yields with high quality. It is time efficient and well worth the money."

Review date: 14 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Illinois



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great value for the money, and saves time!
Rating: 5.0

  • Application Area:Plasmid extraction and cloning

"Miniprep kit from Zymo is great because it takes less time compared to some other kits. The yield and purity of plasmid prepared with this kit are good and comparable to other brands."

Review date: 13 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: IBBR/CNR



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    4 out of 5
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Great results with the right price.
Rating: 4.0

  • Application Area:Quick preparation of plasmid DNA

"Very easy procedure starting with very low amount of cells. Surprising DNA yield with very good quality for the next experimental steps (260/280 OD value, sequencing, restriction analysis, PCR, etc...)."

Review date: 13 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: GigaGen



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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High yields, great price
Rating: 5.0

  • Application Area:Plasmid preparation

"This plasmid prep kit works great. We get higher yields than with the QIAGEN kit, it is easier and also cheaper. Zymo Tech Support is also helpful. "

Review date: 13 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of New Mexico Health Science Center



  • Ease of use
    5 out of 5
    After sales service
    3 out of 5
    Value for money
    5 out of 5
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Awesome kit!
Rating: 4.3

  • Application Area:Plasmid DNA purification

"We love this product! It's fast, reliable and always produces clean and high quality DNA. This kit is way cheaper than competitor brand. We have basically transitioned all our plasmid DNA isolation kits to Zymo products!"

Review date: 13 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: CEITEC MU



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product and good price-quality relationships
Rating: 5.0

  • Application Area:Plasmid DNA isolation

"Cost effective. Quick protocol and Good yield. DNA is of good quality for further cloning procedures."

Review date: 13 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Augustana College



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Excellent results from a fast kit
Rating: 5.0

  • Application Area:Isolate plasmid DNA from bacterial samples

"This product and fast and easy to use. My undergraduate students had no problems following the short protocol to obtain high quality plasmid DNA. I would definitely purchase this kit again."

Review date: 13 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Rosalind Franklin University of Medicine and Science



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great performance. Easy protocol. Transfection quality plasmid DNA.
Rating: 5.0

  • Application Area:Plasmid DNA purification

"Great performance! The pellet-free modified alkaline lysis procedure is very cool and fast! The protocol is relatively easy and straight forward. Transfection quality plasmid DNA. I have switched to this product a couple of months back and am happy about it!"

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Icahn School of Medicine at Mount Sinai



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Really great- have switched to Zymo for all kits
Rating: 5.0

  • Application Area:purify plasmid DNA from bacterial culture for sequencing and/or transfection

"The product is easy, reliable and efficient. The cost per column is also significantly better than QIAGEN, although Zymo recently has raised their prices. I like how you can go directly from culture in this kit, although I never use just 600ul (typically I spin 5mL of culture down and resuspend in PBS). "

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2012

  • Organization: Academia Sinica



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Best mini-prep kit in the market
Rating: 5.0

  • Application Area:cloning and gene manipulation

"Compare to other company's product, it is cheaper. Easy to use. If you have many samples, it can save you a lot of time."

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: academia



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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It's the go-to kit for my lab!
Rating: 5.0

  • Application Area:purify plasmids, bacterial dna preparation

"It is easy to use, protocol is direct and easy to follow, and the purification of the plasmids always results in a high quality. Due to the time it takes to use the kit and the ease of instructions I always teach my undergraduate students how to perform MiniPreps using this kit only! It's approachable and quick! Love it!"

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Northeastern University



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Love it
Rating: 5.0

  • Application Area:large prep enzyme production

"High quality reproducible preps, easy to use, quick "

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Tennessee



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Love this kit! Very quick and easy to use.
Rating: 5.0

  • Application Area:DNA Isolation/purification

"Love this kit! Very quick and easy to use. We receive good quality DNA which we use for qPCR."

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: NCI, NIH



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Great results, I would use it again
Rating: 4.3

  • Application Area:plasmid isolation from 4 ml bacterial cultures

"It’s easy to use, offers great yield and quality DNA"

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Umich



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Great product!
Rating: 5.0

  • Application Area:Growing CRISPR plasmids for transfection.

"Fantastic! Have used it for 5 months with no issues. Equivalent to QIAGEN and much cheaper! "

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: Clark University



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    5 out of 5
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Very efficient and easy to use. High quality results obtained reproducibly.
Rating: 4.7

  • Application Area:Miniprep of plasmid isolates

"The Miniprep kit is very user friendly and easy to use. Gave me very high quality results and a pretty good yield of DNA which I easily used for cloning and other downstream applications. The kit is also very less prone to contaminants and involves fewer cumbersome steps as often found in other Miniprep kits. So thank you very much."

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2016

  • Organization: University of Michigan



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Fastest kit, consistent and great results
Rating: 5.0

  • Application Area:DNA preparation

"I love all Zymo kits. They are fast, easy to use and reliably give good results."

Review date: 12 Jul 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Advanced Reviewer

  • Member since: 2015

  • Organization: The Institute of Cancer Research



  • Ease of use
    5 out of 5
    After sales service
    5 out of 5
    Value for money
    5 out of 5
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Glad to know this brand from my colleague, save lots of time and still give me very good quality and yield of plasmids
Rating: 5.0

  • Application Area:Plasmid preparation

"Easy to use, quick procedure without any incubation time, good yield and quality for further experiments. Good value. Highly recommend."

Review date: 23 May 2016 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2015

  • Organization: The Ohio State University



  • Ease of use
    5 out of 5
    After sales service
    2 out of 5
    Value for money
    5 out of 5
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Great product. The savings mean we will never go back.
Rating: 4.0

  • Application Area:Make plasmids for protein expression

"These kits work great and are easy to use. The DNA is as clean if not cleaner than the "name brand" kit we used to use. The price is a huge bonus. We won't go back."

Review date: 24 Nov 2015 | Zyppy™ Plasmid Miniprep Kit
  • Status:

    Reviewer

  • Member since: 2010

  • Organization: Texas A&m Health Science Center



  • Ease of use
    5 out of 5
    After sales service
    4 out of 5
    Value for money
    4 out of 5
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Rating: 4.3
"fast, east, good yield."

Review date: 05 Jan 2011 | Zyppy™ Plasmid Miniprep Kit

The Zyppy™ Plasmid Miniprep Kit features a pellet-free modified alkaline lysis method that bypasses bacterial culture centrifugation and resuspension steps common to classical plasmid preparation procedures.

Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify using the provided Fast-Spin column technology. Additionally, the innovative colored buffers included in the kit permit error-free visualization and identification of complete bacterial cell lysis and neutralization. The Zyppy™ Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. coli efficiently.

The plasmid DNA is of the highest quality, endotoxin-free, and is well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.

 

Product Overview

Zyppy™ Plasmid Miniprep Kit by Zymo Research product image

Zyppy™ Plasmid Miniprep Kit

Manufacturer Zymo Research

4.7 / 5.0 | 81 reviews